Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_vch_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_vch_pgi 1299499–1300697 1300697 1–1199 29 [0] [0] 29 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 12994980 (0.000)29 (1.630) 27/280 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_vch_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTG  >  NC_000913_3_vch_pgi/1299357‑1299641
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aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                          <  1:40978‑M1/149‑8 (MQ=255)
      actgagttattagGATTTCCTTATTTGCTTTTCCTTTACATTATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTCTGCTACAGGTAAGTGCTAAataataatcaatt                                                                                                                                    <  2:101375‑M1/145‑14 (MQ=255)
               taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAATTGCTAAataataatcaattgttaattta                                                                                                                           >  2:243145‑M1/1‑127 (MQ=255)
                 aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                         <  1:297919‑M1/149‑25 (MQ=255)
                       tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                                                                                                                   >  1:185303‑M1/1‑119 (MQ=255)
                         cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                 <  1:184325‑M1/149‑33 (MQ=255)
                                    ctcctcGCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATGCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                      <  2:188929‑M1/149‑44 (MQ=255)
                                                 tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                         >  2:117540‑M1/1‑93 (MQ=255)
                                                  gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGTAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                        >  2:279471‑M1/1‑92 (MQ=255)
                                                           gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                               >  1:171914‑M1/1‑83 (MQ=255)
                                                                 tcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatccttag                                                                         >  1:136812‑M1/1‑77 (MQ=255)
                                                                            ggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattttactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                              <  2:99371‑M1/149‑84 (MQ=255)
                                                                            ggCTTGATGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaatactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                              <  2:99557‑M1/149‑84 (MQ=255)
                                                                                     aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                     >  1:176353‑M1/1‑57 (MQ=255)
                                                                                               gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatg                                           >  1:199119‑M1/1‑47 (MQ=255)
                                                                                                aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                        >  2:164967‑M1/1‑46 (MQ=255)
                                                                                                aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                        <  1:164967‑M1/135‑90 (MQ=255)
                                                                                                       attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                   <  1:183777‑M1/149‑111 (MQ=255)
                                                                                                        ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                  >  2:287701‑M1/1‑38 (MQ=255)
                                                                                                         tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattcttgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                 >  1:176837‑M1/1‑37 (MQ=255)
                                                                                                                  cACTGCCTTTGGAAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgc                        <  2:220031‑M1/149‑122 (MQ=255)
                                                                                                                     tGCCTTTGGTAGAGGAAAGTGCTAActaataatcaattgttaaattgttgtgcatttcactactggacctgtaatcagaaaagacagacatgcttagccaatctctatttgattgaattgaaaaatgtttgttaaggcatgggtgcaag                     >  1:292775‑M1/1‑25 (MQ=255)
                                                                                                                      gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                    <  1:122390‑M1/149‑126 (MQ=255)
                                                                                                                             gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatagaattgaaagatttttattaatgcatggaggccacctatacat             >  2:113127‑M1/1‑17 (MQ=255)
                                                                                                                               agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc           <  1:199203‑M1/149‑135 (MQ=255)
                                                                                                                                gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcggaaaagatagacatgcttagccaatctctattacattgaagtgaaagatgtttgttaaagcatggatgcaagagttagattct          >  1:85408‑M1/1‑14 (MQ=255)
                                                                                                                                gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgaaactgtaatcagaaaatatagacatgcatagccaacaactatttaattgaattgaaaaatgtttgttaaggcatcgatgcaagctgtagattct          >  2:140252‑M1/1‑14 (MQ=255)
                                                                                                                                  ggAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga        <  1:221222‑M1/149‑138 (MQ=255)
                                                                                                                                        tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt  >  1:118390‑M1/1‑6 (MQ=255)
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AATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTG  >  NC_000913_3_vch_pgi/1299357‑1299641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: