New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	= 1872229	23 (1.040)	3 (0.140)	3/270	2.9	12.0%	coding (189/1491 nt)	yeaJ	putative diguanylate cyclase
	?	NC_000913_3_vch_pgi	= 1872232	22 (1.040)					coding (192/1491 nt)	yeaJ	putative diguanylate cyclase
Rejected: Frequency below/above cutoff threshold.

TGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGC  >  NC_000913_3_vch_pgi/1872085‑1872364                                                                                                                                                     |                                                                                                                                     tgtCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGa                                                                                                                                     >  2:369797‑M2/1‑148 (MQ=255)      ctctgctcACATCTTCCTTCCATATCTTTCAACTTGCCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGatgcgc                                                                                                                                >  1:85459‑M2/4‑143 (MQ=255)            tGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGAATCCTCCTGTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCtctc                                                                                                                          >  1:348648/1‑149 (MQ=255)                            tATGTTTGGAGTTGTCGCCAGTGACAGGGCAATGAGTGCCTGTCTG‑CGCTATATCGTGCTGAAAGCAGTCTGCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGTGCTCTCGCTGCTGAGCTGTCgg                                                                                                          <  2:26441/149‑1 (MQ=255)                              tttttGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCCCTCGCTGCTGAGCAGTCGGaa                                                                                                        >  2:2369/1‑149 (MQ=255)                               tttgcaaCTTGTCGCGAGCGAAAGAGCAATGAGTGCCGAACTG‑CGCGAAATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGACTCCGAGGATTGCCGCGCATGGGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAg                                                                                                       <  1:175810/144‑1 (MQ=255)                                    aaCTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCg                                                                                                  >  1:199629/1‑149 (MQ=255)                                                   aCAGCGCAATGAGTGCCTTTTTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGgcgc                                                                                   <  1:162500/149‑1 (MQ=255)                                                     agagCAATGAGTGCCTAGCTGACGC‑ATATCGTTCAGAAAGCAGACTCCTCCTTTCTTTATGAGAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGgcgcgc                                                                                 <  2:63027/149‑1 (MQ=255)                                                                              tataTGGCGCAGAAAGCAGACTTCTCTTTTCTTTATGATAAGTATCAGAATCAGAGTATCGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGGCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGc                                                         <  2:131898/149‑1 (MQ=255)                                                                                    gTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGt                                                             >  1:94151/1‑139 (MQ=255)                                                                                    gTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGt                                                             <  2:94151/139‑1 (MQ=255)                                                                                     tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCTGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATagc                                                  >  2:87613/1‑147 (MQ=255)                                                                                                     ctcctTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTAAGTCTGCCAATAACACCCATGGCTCAAAcc                                  >  2:57565/1‑149 (MQ=255)                                                                                                             tatATGATAAGTATCAGAATCAGACTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGcc                          <  1:170986/147‑1 (MQ=255)                                                                                                                 tGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGatgcgcggcaatactctgattctgatacttatcataaagaaaggaggagtctgctttctgcccgagatagcgcagataggcactcattgctctgtcgctggcgacaagttca                      <  1:227417‑M2/149‑113 (MQ=255)                                                                                                                  gATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATaa                     >  1:325215/1‑149 (MQ=255)                                                                                                                   aTAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATaaa                    >  2:372685/1‑149 (MQ=255)                                                                                                                     aaGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAacac                                                >  2:201742/1‑119 (MQ=255)                                                                                                                     aaGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAacac                                                <  1:201742/119‑1 (MQ=255)                                                                                                                        tATCAGAATCAGAGTATTGCCGCGCATGTGatgcgcggcaatactctgattctgatacttatcataaagaaaggaggagtctgctttctgcacgatatagcgcagataggcactcattgctctgtcgctgg                                 >  1:6325‑M2/1‑30 (MQ=255)                                                                                                                        tATCAGAATCAGAGTATTGCCGCGCATGTGatgcgcggcaatactctgattctgatacttatcataaagaaaggaggagtctgctttctgcacgatatagcgcagataggcactcattgctctgtcgctgg                                 <  2:6325‑M2/131‑102 (MQ=255)                                                                                                                        tATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATAccc               <  2:348648/149‑1 (MQ=255)                                                                                                                          tcagaatcagaGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCgg             <  1:168562/149‑1 (MQ=255)                                                                                                                          tcagaatcagaGTATGGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCgg             <  1:169193/149‑1 (MQ=255)                                                                                                                                   gagTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTAcgcg    <  1:386698/149‑1 (MQ=255)                                                                                                                                     gTAGTGCCGGGCATGGGATGGGCGCTGGCGCTGCTGAGAAGTCGGAGGTGTCGCGAGAGCAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCAGAAATACCCGGGCTTACGCGGc  <  2:181335/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                     TGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTG‑CGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGC  >  NC_000913_3_vch_pgi/1872085‑1872364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.