New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 417180322 (1.000)4 (0.190)
+TTGATGT
4/276 2.5 16.7% intergenic (+47/‑254) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
?NC_000913_3_vch_pgi = 4213155 20 (0.910)noncoding (116/120 nt) rrfE 5S ribosomal RNA of rrnE operon
Rejected: Frequency below/above cutoff threshold.

TCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGC  >  NC_000913_3_vch_pgi/4171661‑4171941
                                                                                                                                              |                                                                                                                                          
tCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGGGCGAGTAGGGAACTGCCAGACATCAAATAACACGAAAGGCTAAGTAGAAGGCCTGGGCTTTTCGGTTTGTttgttg                                                                                                                                      >  2:336786/1‑149 (MQ=11)
                       tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGa                                                                                                               >  2:259623/1‑149 (MQ=35)
                       caaGAAGTGAAACGCCGTAGCGCCGATCGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCAGCAAATAAAACGAAAGGCTCACTCGCACAACAGGGCCTTTCGCTTTATCTGTCGTTTGTCGGTGAACGCTCTCCTGa                                                                                                               >  2:281283/3‑149 (MQ=11)
                             gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGa                                                                                                         >  2:76411/1‑149 (MQ=35)
                                      cGTAGCGCCGATGGTAGGGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATccgc                                                                                                <  1:9253/149‑1 (MQ=25)
                                              cGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCg                                                                                        >  2:363098/1‑149 (MQ=35)
                                                          ggggTCTCCGCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGtt                                                                            <  1:146003/149‑1 (MQ=17)
                                                           gggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAGGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTGCTGAGTAGGACAAATCCGCCGGGAGCGGGTTTTAACGTTg                                                                           >  2:215080/1‑149 (MQ=255)
                                                             gTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCg                                                                         <  1:111543/149‑1 (MQ=35)
                                                               ctcCCCATGCGATAGTAGGGAACTGCCAGGCATCAAATAAAACGAACGGCTCAGTCGAAAGACTGGGCCTTTCGTGTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGaa                                                                       <  1:144536/149‑1 (MQ=255)
                                                                          agagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCg                                                            <  2:68846/149‑1 (MQ=32)
                                                                            agTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGa                                                          <  1:293983/149‑1 (MQ=32)
                                                                                 ggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAt                                                                                                    >  2:382073/1‑102 (MQ=33)
                                                                                 ggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAt                                                                                                    <  1:382073/102‑1 (MQ=33)
                                                                                                     aaaaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCAt                                 <  1:363098/149‑1 (MQ=32)
                                                                                                      aaaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg                                                       >  2:202158/1‑126 (MQ=33)
                                                                                                      aaaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg                                                       <  1:202158/126‑1 (MQ=33)
                                                                                                      aaaCGAAAGGCTCAGTCGAAAGACTGAGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgc                                >  1:324995‑M1/1‑41 (MQ=255)
                                                                                                        aCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATctgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttca                                                                       <  1:143174‑M1/108‑70 (MQ=255)
                                                                                                        aCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATctgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttca                                                                       >  2:143174‑M1/1‑39 (MQ=255)
                                                                                                                cTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAgg                      >  1:316462/1‑149 (MQ=32)
                                                                                                                   aGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCAt                   <  1:56693/149‑1 (MQ=32)
                                                                                                                    gTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATc                  <  1:369410/149‑1 (MQ=32)
                                                                                                                     tCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTa                                                                                                            >  2:321364/1‑58 (MQ=35)
                                                                                                                     tCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTa                                                                                                            <  1:321364/58‑1 (MQ=38)
                                                                                                                                    ttttGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcc  >  2:256867‑M1/1‑11 (MQ=255)
                                                                                                                                              |                                                                                                                                          
TCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGC  >  NC_000913_3_vch_pgi/4171661‑4171941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.