New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 2025238	51 (1.200)	4 (0.100)	4/266	5.7	7.7%	noncoding (76/87 nt)	dsrA	sRNA antisense regulator enhances translation of RpoS, Hfq‑dependent
	?	NC_000913_3_hsa_tpiA	= 2025243	49 (1.240)					noncoding (71/87 nt)	dsrA	sRNA antisense regulator enhances translation of RpoS, Hfq‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATA  >  NC_000913_3_hsa_tpiA/2025105‑2025388                                                                                                                                           |                                                                                                                                                   ttCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGTATGCACACTCAAAAAATCCCGCACCTGAGGGGGACGGGAt                                                                                                                                          >  1:100364/1‑149 (MQ=255)   cTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGa                                                                                                                                        >  1:407903/1‑149 (MQ=255)     gAACACGTTAGCGTCGATGGCCGTGAATATCATACATGGCGCGAATTGCCGGATGCATACAAACTGGATATTGATGACTTCAGCGCCACCGAAGCGAAACCATGAATGCACCAAAAAAAAACACCGACCCTGAGGAGGACgggcacaaa                                                                                                                                      >  2:421284/1‑143 (MQ=255)     gAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGaaa                                                                                                                                      <  1:741908/149‑1 (MQ=255)         acGGTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTg                                                                                                                                  <  1:23036/149‑1 (MQ=255)          cGTTAGCGTCGATGGACGTGAATAGCATACATGGCGGGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTGCAGCGTCTCTGAAGTGAATCGGTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGc                                                                                                                                 <  1:113280/149‑1 (MQ=255)                         aCGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc                                                                                                                  <  1:222549/149‑1 (MQ=255)                                tatCATACATGGCGTGACGTGGCGGATGCCTTCGAACTGGATATTCATGGCTTCAGCGTCTCTTAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACACTGAGGTGGTGGGAGTGAAACTTGCTTACGCAAGCAGAACTTcaaga                                                                                                           >  2:343895/1‑147 (MQ=255)                                 atCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTaaaaaa                                                                                                          >  2:541543/1‑149 (MQ=255)                                   cATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCGTGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAAtt                                                                                                        >  1:618787/1‑149 (MQ=255)                                    aTACACGGCGAGATTTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGGCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTc                                                                                                       >  2:659314/1‑149 (MQ=255)                                        atcgCGAGAACTGGCGGAAGCCCACGAACTGGACATCCATTACTTCAGCATCTCTCAAGTGAATCGTTGAACGCACAATAAAAAAATCCCCACCCTGAGGGGGTCGGGATCAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTa                                                                                                   <  2:46515/146‑1 (MQ=255)                                        aTGGGGTGAATTGGAGGATGCCTTCGAACTGGATAGTCATGATTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTACAAAATTCGttt                                                                                                   <  2:525102/149‑2 (MQ=255)                                         tGGCGTGAATGGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGGGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTac                                                                                                  <  1:299334/149‑1 (MQ=255)                                             gTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCCCTTCAAAAATTCGTTACAccg                                                                                              >  2:184085/1‑148 (MQ=255)                                             gTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCa                                                                                              <  2:407903/149‑1 (MQ=255)                                              tGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAg                                                                                             >  1:75760/1‑149 (MQ=255)                                                       ggATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGCTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGa                                                                                    <  2:650054/149‑1 (MQ=255)                                                        gATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAt                                                                                   <  1:617035/149‑1 (MQ=255)                                                          tGCCTTCGAAATGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAtgt                                                                                 <  1:308304/149‑1 (MQ=255)                                                              ttCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGATTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAtgcattc                                                                             >  2:548138/1‑144 (MQ=255)                                                              ttCGAACTGGATATTCATGACTTCAGCGTCTCTAAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGAGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCTTTACAACAGGAAATCGAATATGTtc                                                                             >  2:20676/1‑149 (MQ=255)                                                              ttCGAACTGAATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCCACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGACGCACTTAAAAAATTCGTTACACCCGGAAATCTGATGCGTtc                                                                             >  2:101482/1‑149 (MQ=255)                                                                    cTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCAcc                                                                       >  1:564231/1‑149 (MQ=255)                                                                       gATATTCATGACTTCAGCGACTCTGAAGTGAATCGTTGACTGCACAATAAAAAAATCCCGACCCTGAGGGAGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTAAACCAGGAAATCTTATGTGTTCACCACCtcg                                                                    >  2:724059/1‑147 (MQ=255)                                                                          atTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTCATGAGTTCTTAACCTTATcc                                                                 >  1:665404/1‑149 (MQ=255)                                                                          atTCATGAATTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATcc                                                                 <  1:285854/149‑1 (MQ=255)                                                                                   ttCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttt                                                        >  1:295579/1‑149 (MQ=255)                                                                                   ttCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttt                                                        >  2:641306/1‑149 (MQ=255)                                                                                        cGTCTCTGACGTGAGTGTGTAAATGAAGAATAAAAAAGTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGc                                                   <  2:340818/149‑1 (MQ=255)                                                                                            tctGAAGTGAATCGTTGAATGCCCAAAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTAAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATAACCTCATCAGCAATTTTTTTCGCGGAc                                               >  2:151826/1‑149 (MQ=255)                                                                                              tGAAGTGGATCGTTGAATGCAAAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACa‑a                                            <  1:637827/149‑1 (MQ=255)                                                                                                aaGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGa                                          >  1:52569/1‑149 (MQ=255)                                                                                                     aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCaa                                                             >  2:444154/1‑126 (MQ=255)                                                                                                     aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCaa                                                             <  1:444154/126‑1 (MQ=255)                                                                                                     aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAata                                     <  1:19428/149‑1 (MQ=255)                                                                                                        cGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTc                                  <  1:702097/149‑1 (MQ=255)                                                                                                          ttGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACAACAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGc                                <  1:594956/149‑1 (MQ=255)                                                                                                            gAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCa                              >  2:565518/1‑149 (MQ=255)                                                                                                               tGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCACTGATACAGAAGAT‑TTCGCCatat                           >  2:117544/1‑149 (MQ=255)                                                                                                                  acaATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATatgaa                        <  2:295579/149‑1 (MQ=255)                                                                                                                      tAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGATA‑AGAAAATATTCGCCATatgaatgct                    >  2:614692/1‑147 (MQ=255)                                                                                                                         aaaaaTCCCGACCCTGAGGGGGTCGGGACGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTaa                 <  1:172345/149‑1 (MQ=255)                                                                                                                           aaaTCACGACCCTGAGGGGGTCAGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACg               <  2:225751/149‑1 (MQ=255)                                                                                                                                 cGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAACTTCGTTCAAACTAGAAATGTGCCGTGTGCATCACCTTATCCGCAATGTTTATCGCTGACA‑AGAAAGTATTCGCCATATGAATGATTAACGCTACtt         >  1:177894/1‑149 (MQ=255)                                                                                                                                 cGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACtt         >  1:513991/1‑149 (MQ=255)                                                                                                                                   aCCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTAt       <  2:703364/149‑1 (MQ=255)                                                                                                                                     ccTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGa     <  1:550799/149‑1 (MQ=255)                                                                                                                                      cTGAGGGGGTCGGGCTGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCAACTTATGaa    >  2:177791/1‑149 (MQ=255)                                                                                                                                      cTGAGGGGGTCGGGATGAAACTTGCCTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGaa    <  1:390134/149‑1 (MQ=255)                                                                                                                                        gAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATa  <  1:541543/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                   TTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATA  >  NC_000913_3_hsa_tpiA/2025105‑2025388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.