Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503	1979278	776	44 [0]	[0] 46	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	43 (1.070)	32/270	0.3	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAA  >  NC_000913_3_hsa_tpiA/1979135‑1979427                                                                                                                                                 |                                                                                                                                                     atgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATaa                                                                                                                                                  <  2:512851‑M2/5‑1 (MQ=255)      tctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcgcatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacgctcaattacttaacatagatgTATAAGTCAt                                                                                                                                             >  2:191743‑M2/140‑149 (MQ=255)         ttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACt                                                                                                                                          >  1:573474‑M2/137‑149 (MQ=255)             acttatctaagatttttcctaaatcgacgcaactgtcctcgtcactatacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaaccctcaattacttaacataaattTATAAGTCATActtttt                                                                                                                                      >  2:463273‑M2/133‑148 (MQ=255)                 ctcgaagatttttcctaaatcgacgcaactgtactcgtcactacacgcccatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtttt                                                                                                                                  <  1:465694‑M2/21‑1 (MQ=255)                 atcaaagagtgttccgaaatggacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgggatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtttt                                                                                                                                  <  2:256401‑M2/21‑1 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATACGTCATACTCTTGTTCTggg                                                                                                                               >  2:5369‑M2/126‑149 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                               >  1:386967‑M2/126‑149 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgaggcagatcacacaaaacactcaattacttaacataactgCATAAGTCAtacttttgtttttgc                                                                                                                               >  2:671503‑M2/126‑146 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcaaatactacggaggagggctgcgattttcaataatgcgtgatgcagatcccacaaaacactcaattacttaacataaatgTATAAGTTATACTTTTGTTTTggg                                                                                                                               >  2:124650‑M2/126‑149 (MQ=255)                    taagatttttcctaaatcgacgcaactgacgatattccaagacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagataacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                               >  2:162738‑M2/126‑149 (MQ=255)                     aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                                                                              >  1:74029‑M2/125‑149 (MQ=255)                            ttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                                                                       >  1:93494‑M2/118‑149 (MQ=255)                               ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGTGGTATTTCAAt                                                                                                                    >  2:265477‑M2/115‑149 (MQ=255)                               ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                    >  2:584280‑M2/115‑149 (MQ=255)                               ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacaatcaattacttaacataaattTATAAGTCATACTTTTGTTTTGTTTGTATTTCAAt                                                                                                                    >  2:11816‑M2/115‑149 (MQ=255)                                taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                                                                   >  2:674423‑M2/114‑149 (MQ=255)                                   atcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaaaactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGAGTGTATTTCAATCTGt                                                                                                                >  1:664496‑M2/111‑149 (MQ=255)                                    tcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgagtttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCtttt                                                                                                               >  1:129164‑M2/110‑149 (MQ=255)                                           aactgtactcgtcactacacgcacatacaacgggggggggctgcgatattcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATTTGTTAAAAAGt                                                                                                        >  1:218404‑M2/103‑149 (MQ=255)                                                   tcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTAATACTTTTGTTTTGGTTTTATTTCAATCTGTTAAAAAGTTTTTcgct                                                                                                >  2:149595‑M2/95‑149 (MQ=255)                                                          tacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTAAAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTag                                                                                         >  1:111487‑M2/88‑149 (MQ=255)                                                             acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                      >  2:248084‑M2/85‑149 (MQ=255)                                                                    tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                               <  2:93494‑M2/72‑1 (MQ=255)                                                                     acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCGGTTAAAAAGTTTTTCGCTCCGCTAGCCATCAAAAAt                                                                              >  2:25381‑M2/77‑149 (MQ=255)                                                                        acggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaa                                                                           <  2:380858‑M2/76‑1 (MQ=255)                                                                          ggagggcggctgcgattttcaataatgagtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTAATACTTTTGTTTTGGGTGTATTTCAATCTGTTAGAAAGTTGTTCGCTACGCTAGCACGCACACAtgaaca                                                                         >  2:281067‑M2/72‑147 (MQ=255)                                                                               ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                                    <  2:74029‑M2/83‑1 (MQ=255)                                                                                   ctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                                >  1:604448‑M2/63‑149 (MQ=255)                                                                                   ctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                                >  1:31892‑M2/63‑149 (MQ=255)                                                                                    tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAATTTTGTTTTGGGTGTATTTCAATCTGTTAAAACGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATc                                                               >  1:588460‑M2/62‑149 (MQ=255)                                                                                          tatcaataatgcgtgacgcagatccaacaaaaaactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATg                                                         <  1:625878‑M2/94‑1 (MQ=255)                                                                                                  atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGATAGCAAGCAAAAATGAAACAGGAATAATCGAAAGGGGATGTTg                                                 >  1:635002‑M2/48‑149 (MQ=255)                                                                                                      gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                             <  1:248084‑M2/106‑1 (MQ=255)                                                                                                      gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                             <  1:134311‑M2/106‑1 (MQ=255)                                                                                                               atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACCGACaaaa                                    >  1:620384‑M2/35‑149 (MQ=255)                                                                                                               atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                                    >  2:407046‑M2/35‑149 (MQ=255)                                                                                                                tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCCCAGTCAAAAt                                   >  1:418829‑M2/34‑149 (MQ=255)                                                                                                                 cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAACAACAATGAAACAGGAGTAATCGAAATGAACAGTTGCGCAATGtcataaaa                                  >  2:21944‑M2/33‑147 (MQ=255)                                                                                                                    acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                               >  1:201930‑M2/30‑149 (MQ=255)                                                                                                                    acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                               >  1:695449‑M2/30‑149 (MQ=255)                                                                                                                      aaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCa                             <  1:481607‑M2/122‑1 (MQ=255)                                                                                                                                        acataaatgTATAAGTCACACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGc           <  1:276782‑M2/140‑1 (MQ=255)                                                                                                                                           taaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCtat        <  1:72593‑M2/143‑1 (MQ=255)                                                                                                                                                 tataAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACAGTAAATAATCATCTGCTataaataa  >  2:462690/1‑149 (MQ=255)                                                                                                                                                 tataAGTCATACTTTTGTTTTGGGTGTATTTCAAACTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaataa  <  1:531800/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAA  >  NC_000913_3_hsa_tpiA/1979135‑1979427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑