Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503	1979278	776	56 [0]	[0] 56	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	52 (0.820)	45/264	0.5	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

GTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_hsa_tpiA/1978363‑1978648                                                                                                                                            |                                                                                                                                                   gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcat                                                                                                                                          >  2:174824‑M1/1‑140 (MQ=255)    tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATAACACACAACACTCAATTACTTAACATCAATAtataaggcatact                                                                                                                                       >  2:63439‑M1/1‑137 (MQ=255)     ttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCGCATACAACGGAGGGGGGCTGCGATTTTCAATAATTCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtctaagtcatactt                                                                                                                                      >  2:299841‑M1/1‑136 (MQ=255)          cTTATCTAAGATTTTTTCTTTTTTTTCGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACTCTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                                 >  2:611145‑M1/1‑131 (MQ=255)           ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt                                                                                                                                <  1:101718‑M1/150‑21 (MQ=255)                          ccTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAACCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                 <  1:928857‑M1/150‑36 (MQ=255)                           cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                                                                >  1:23910‑M1/1‑114 (MQ=255)                               aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACATAAAACACTCAATTACTTAACATAAAGGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                                                                            <  2:445014‑M1/150‑41 (MQ=255)                                           gTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                                >  1:931002‑M1/1‑98 (MQ=255)                                                 gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacc                                                                                          >  2:627217‑M1/1‑92 (MQ=255)                                                    aCTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                       <  1:284294‑M1/150‑62 (MQ=255)                                                     cTACACGCACATACAACGCAGGGGGGCTGCGATTTGCAATAATGCGTCATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctag                                                                                      <  1:387741‑M1/150‑63 (MQ=255)                                                        cacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                                   <  1:83913‑M1/150‑66 (MQ=255)                                                        cacGCAAATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                                   <  2:774159‑M1/150‑66 (MQ=255)                                                         acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcttcgctagctag                                                                                  >  2:164248‑M1/1‑84 (MQ=255)                                                            cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                               >  1:494588‑M1/1‑81 (MQ=255)                                                             acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaa                                                                              >  1:111626‑M1/1‑80 (MQ=255)                                                               aTACAACGGCGTGGGGCTCCGATTTTCAATAATTCGTGATGCAGATCACACAAACCACTAAATTACTTAACATAAATGtataagtcatacttttgttttgggtatatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                            <  1:525352‑M1/150‑73 (MQ=255)                                                               aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcgaaaa                                                                            >  1:792361‑M1/1‑78 (MQ=255)                                                                tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttatttttggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                                                                           <  1:39356‑M1/150‑74 (MQ=255)                                                                 aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAACACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatg                                                                          <  1:627217‑M1/150‑75 (MQ=255)                                                                   aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctcttaaaaagtttttctctaagctagcacgcaaaaatgca                                                                        >  2:1124369‑M1/1‑74 (MQ=255)                                                                    aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                       >  1:537472‑M1/1‑73 (MQ=255)                                                                       gcggggggctTCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                    >  2:606799‑M1/3‑70 (MQ=255)                                                                          gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttgtgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaa                                                                 <  2:197815‑M1/150‑84 (MQ=255)                                                                             ggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa                                                              <  2:537472‑M1/150‑87 (MQ=255)                                                                              gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagaaaaaatgaaaaaggaataat                                                             >  2:856420‑M1/1‑63 (MQ=255)                                                                                tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAAATCCTTGACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcg                                                           <  1:158023‑M1/150‑90 (MQ=255)                                                                                     ttttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatg                                                      <  1:276565‑M1/150‑95 (MQ=255)                                                                                      tttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgg                                                     <  2:23910‑M1/150‑96 (MQ=255)                                                                                      tttCAAAAATGCGTGATGCAGATCACACAAAACACACAATTACGGAACATAAATGtataagtcatacgttggttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagaaagcaaaaatgaaacaggaataatcgaaatgg                                                     <  1:165245‑M1/150‑96 (MQ=255)                                                                                          aatactGCGTTATGCAGATCACACAAACCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctatcaagcaaaaatgaaacaggaataatcgaaatgggatg                                                 <  1:434619‑M1/150‑100 (MQ=255)                                                                                          aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcacaatgggatg                                                 >  2:1163924‑M1/1‑51 (MQ=255)                                                                                                 ggtGAGGCAGATCACACAAAACACTCAATTACTTATCTTAAATGgataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                          <  1:739125‑M1/149‑107 (MQ=255)                                                                                                   tGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                        >  2:100038‑M1/1‑42 (MQ=255)                                                                                                           aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaat                                >  1:479420‑M1/1‑34 (MQ=255)                                                                                                             cacacaAAACTCTATATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                              <  1:340471‑M1/150‑119 (MQ=255)                                                                                                              acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatggtgagaagagtcaaaataac                             >  1:921344‑M1/1‑31 (MQ=255)                                                                                                              acacaAAACACTCAATTACTTAACATAAATGtataagtaatactttagctttggatgtatatcaatctgttaaaaagtttttctctacgctagcaatcaaaaatgaaacaggaataatcgaaatggcatgttccacacagacaatatgac                             >  2:104662‑M1/1‑31 (MQ=255)                                                                                                                 caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                          <  1:174824‑M1/150‑123 (MQ=255)                                                                                                                  aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                                                                           >  2:45680‑M1/1‑27 (MQ=255)                                                                                                                  aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                                                                           <  1:45680‑M1/100‑74 (MQ=255)                                                                                                                   aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                             <  2:704770‑M1/129‑104 (MQ=255)                                                                                                                   aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                             >  1:704770‑M1/1‑26 (MQ=255)                                                                                                                       acTCAATTACTTAACATAAATTtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                        <  1:1056049‑M1/130‑109 (MQ=255)                                                                                                                       acTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaa                    <  1:611145‑M1/150‑129 (MQ=255)                                                                                                                       acTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                        >  2:1056049‑M1/1‑22 (MQ=255)                                                                                                                        cTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagaaatactcgcaatgggatgttgcgcacagtcaaaataactcaccgtaaa                   >  1:1044002‑M1/1‑21 (MQ=255)                                                                                                                               aCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcat            <  1:606799‑M1/150‑137 (MQ=255)                                                                                                                                 ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatct          >  2:980570‑M1/1‑12 (MQ=255)                                                                                                                                 ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatct          >  1:584908‑M1/1‑12 (MQ=255)                                                                                                                                 ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataaacatct          >  2:306150‑M1/1‑12 (MQ=255)                                                                                                                                      aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctat     >  1:87910‑M1/1‑7 (MQ=255)                                                                                                                                      aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactaaccgtaaataattatctgctat     >  2:920747‑M1/1‑7 (MQ=255)                                                                                                                                        aaaTGaataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataa   >  2:142878‑M1/1‑5 (MQ=255)                                                                                                                                         aaTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa  >  1:1006849‑M1/1‑4 (MQ=255)                                                                                                                                            |                                                                                                                                                   GTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_hsa_tpiA/1978363‑1978648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑