New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	1715908 =	53 (0.880)	4 (0.070)	4/260	8.3	7.9%	intergenic (‑19/+40)	pdxY/tyrS	pyridoxamine kinase/tyrosyl‑tRNA synthetase
	?	NC_000913_3_hsa_tpiA	1715934 =	44 (0.790)					intergenic (‑45/+14)	pdxY/tyrS	pyridoxamine kinase/tyrosyl‑tRNA synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGA  >  NC_000913_3_hsa_tpiA/1715761‑1716049                                                                                                                                                    |                                                                                                                                              aTTAGAAAATTGAACGGTGTTCAGCGGCCAGACTTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAg                                                                                                                                              >  2:233243/1‑149 (MQ=255)  ttAGAACATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAgg                                                                                                                                             <  2:1011164/149‑1 (MQ=255)   tAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAggg                                                                                                                                            <  1:482353/149‑1 (MQ=255)         aTTGAAAGGTGTTCAGCGGCCAGGCGTTCGCGCCCAGGCTGCGCATCGGAAACTCTGCCGAACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTg                                                                                                                                      <  2:440699/149‑1 (MQ=255)            gAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGaga                                                                                                                                   >  1:270718/1‑147 (MQ=255)               ccgtgtTCAGCGGCCAGACGTCCGCGCACAGGCGGCGCATCGCAAACTCTGGCGCACGGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTtct                                                                                                                                <  2:170252/147‑1 (MQ=255)                 gtgtTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCa                                                                                                                              <  1:28564/149‑1 (MQ=255)                    ttCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTc                                                                                                                           <  1:856164/149‑1 (MQ=255)                        gCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTccccc                                                                                                                       >  2:779328/1‑149 (MQ=255)                            cccgACGTTCGCGCCCAGTCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTc                                                                                                                   <  1:1029492/146‑1 (MQ=255)                            ccAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAg                                                                                                                                              >  1:387236/1‑122 (MQ=255)                            ccAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAg                                                                                                                                              <  2:387236/122‑1 (MQ=255)                              aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAttt                                                                                                                                  >  1:230625/1‑132 (MQ=255)                              aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAttt                                                                                                                                  <  2:230625/132‑1 (MQ=255)                               gACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCAc                                                                                                                >  1:857171/1‑149 (MQ=255)                                      gcgcCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGc                                                                                                         <  2:902514/149‑1 (MQ=255)                                      gcgcCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGCGATTTCTCACTCCCGCTTTCCACTTACTga                                                                                                         >  2:955730/1‑148 (MQ=255)                                          cgaGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCatta                                                                                                     <  2:730463/147‑1 (MQ=255)                                                ggggcATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCa                                                                                               <  2:156278/146‑1 (MQ=255)                                                   gcATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCa                                                                                            >  2:625318/1‑149 (MQ=255)                                                     aTCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCaaa                                                                                          <  2:585685/149‑1 (MQ=255)                                                         gAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAACTCAg                                                                                      >  2:359554/1‑149 (MQ=255)                                                               ctGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTa                                                                                <  1:264007/149‑1 (MQ=255)                                                                  ccGCACTGTTACCCGCATGGCCATAAACAACGTCAGACGCGATAGCGTGAATATTTTTCATCATGTGCCCTGTATAAAAACCAGGGGAAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAAtt                                                                             <  1:826762/149‑1 (MQ=255)                                                                    gCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTAACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCt                                                                           >  1:485856/1‑149 (MQ=255)                                                                    gCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTGTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCt                                                                           <  1:955730/149‑1 (MQ=255)                                                                      aCTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCttt                                                                         <  1:625318/149‑1 (MQ=255)                                                                       cTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCtttt                                                                        <  1:228562/149‑1 (MQ=255)                                                                       cTGTTACCCGAATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCtttt                                                                        <  2:334897/149‑1 (MQ=255)                                                                          ttACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTAc                                                                     <  1:752828/149‑1 (MQ=255)                                                                                gCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCATATCAGAAGTTGATTGTTTTTACTGCGAc                                                               >  1:673289/1‑149 (MQ=255)                                                                                gCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGAc                                                               >  1:865160/1‑149 (MQ=255)                                                                                      ccATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTa                                                         >  1:177338/1‑149 (MQ=255)                                                                                              aacGTGAGACTGGATAGCGAGAATATTTTTCAACAAGTTCCCTTTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTaaaa                                                 <  1:199026/149‑1 (MQ=255)                                                                                                  tGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGAc                                             <  2:599217/149‑1 (MQ=255)                                                                                                       cTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAAc                                        <  1:236773/149‑1 (MQ=255)                                                                                                        tGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACa                                       >  1:884762/1‑149 (MQ=255)                                                                                                        tGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACa                                       >  1:884704/1‑149 (MQ=255)                                                                                                         gtatgttgaggATATGTTTCATCATCTGCCGTGGTCAAGAACCAGGGTGAGTGATTTCTCACTCCCCCTTCCCACTGAATGCATTATTTCCGTCAACTCAGACAGTAATTCTTTGTACCGCGACGCAGTAAGGTAAAACGACCAAACAg                                      <  2:632678/142‑1 (MQ=255)                                                                                                          gATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGa                                     <  2:884704/149‑1 (MQ=255)                                                                                                          gATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGa                                     <  2:884762/149‑1 (MQ=255)                                                                                                               cGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtc                                <  2:857171/149‑1 (MQ=255)                                                                                                                gagaATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGAAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACACTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACAAACCAAACGAtct                               >  2:1007225/1‑149 (MQ=255)                                                                                                                      atTTTTCATCATGTTCCCTGTATAAAAACCAGGGGCACTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACACAGTAAGGTAAAAAAACCAAACAGACGAtcttcttct                         >  2:491795/1‑149 (MQ=255)                                                                                                                              tcatGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAaagaa                 <  1:233243/149‑1 (MQ=255)                                                                                                                                atGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                           <  2:486841/137‑1 (MQ=255)                                                                                                                                atGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                           >  1:486841/1‑137 (MQ=255)                                                                                                                                  gTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTTATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTAt             >  2:284392/1‑149 (MQ=255)                                                                                                                                     cacTGTATAAAAACCAGGGGGACTGATTTCTCACCCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCa          <  1:284608/147‑1 (MQ=255)                                                                                                                                       cTGTATAAAAACCAGGGGGAGTGATTTCTCACCCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAgg        >  2:312917/1‑149 (MQ=255)                                                                                                                                          tataAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAAAAGTATTCAGGacc     >  1:900735/1‑147 (MQ=255)                                                                                                                                           ataAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTGATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCg    <  2:84450/149‑1 (MQ=255)                                                                                                                                             aaaaaCCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGa  <  2:851011/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              ATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGA  >  NC_000913_3_hsa_tpiA/1715761‑1716049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.