Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,774,576 G→A G263E (GGG→GAG)  ydiB → quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,774,5760GA98.6% 206.1 / NA 70G263E (GGG→GAG) ydiBquinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (40/28);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTG  >  NC_000913_3_hsa_tpiA/1774430‑1774719
                                                                                                                                                  |                                                                                                                                               
atCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgagg                                                                                                                                               <  2:596731/149‑1 (MQ=255)
atCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgagg                                                                                                                                               <  2:161910/149‑1 (MQ=255)
    ttGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGa                                                                                                                                           >  1:171791/1‑149 (MQ=255)
          aaTGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          >  1:287042/1‑144 (MQ=255)
          aaTGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          <  2:287042/144‑1 (MQ=255)
           aTGATCTCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTc                                                                                                                                    <  1:43160/149‑1 (MQ=255)
           aTGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTc                                                                                                                                    <  2:604442/149‑1 (MQ=255)
                       tGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACt                                                                                                                        >  2:252696/1‑149 (MQ=255)
                       tGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACt                                                                                                                        >  2:1013598/1‑149 (MQ=255)
                         ttACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTgg                                                                                                                      >  1:136809/1‑149 (MQ=255)
                         ttACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATAGACTgg                                                                                                                      >  1:421726/1‑149 (MQ=255)
                           aCATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACCAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTTTGGGCTTGCa                                                                                                                    >  2:385961/1‑149 (MQ=255)
                             aTCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCaaa                                                                                                                  >  1:87866/1‑149 (MQ=255)
                              tCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCCAAg                                                                                                                 >  1:834971/1‑149 (MQ=255)
                                 gggACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGAtt                                                                                                              >  1:282915/1‑149 (MQ=255)
                                    aCTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTcc                                                                                                           <  2:107865/149‑1 (MQ=255)
                                           gTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCCAAACGATTGATGGATACGGCATGTTGTTGTGGCACGAGGCTGAACAGTTCACATTATGGACTGGCAAGGATTTCCCCCTGGa                                                                                                    >  2:300471/1‑149 (MQ=255)
                                           gTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTACGGACTGGCAAAGATTTCCCTCTGGa                                                                                                    >  2:565080/1‑149 (MQ=255)
                                             cACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCctc                                                                                                        >  2:885562/1‑143 (MQ=255)
                                             cACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCctc                                                                                                        <  1:885562/143‑1 (MQ=255)
                                                tGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGAAACAAGCTGGTTGCAAAACGATTGATAGATACAGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAATCATTTCCCTCTGGAATTTg                                                                                               >  2:282152/1‑149 (MQ=255)
                                                       gtgtATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATCGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACa                                                                                        >  2:280201/1‑149 (MQ=255)
                                                           ataACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTc                                                                                    >  1:421206/1‑149 (MQ=255)
                                                              tcccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAGGTTGTCGTGGCTAGATCGTGAACAGTTCACATTATCGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATg                                                                                 <  1:475292/148‑1 (MQ=255)
                                                              aCCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATg                                                                                 <  2:136809/149‑1 (MQ=255)
                                                               cccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATgg                                                                                <  2:762656/149‑1 (MQ=255)
                                                                        gACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAAACGGTCATGGGGTTCGGTg                                                                       >  2:482279/1‑149 (MQ=255)
                                                                           gAAGTTACTGCAGCAGACGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCCTGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTCGAATAGGGTAAACAGGTCATGGGGGGCGGTGCCt                                                                    >  2:129231/1‑149 (MQ=255)
                                                                              gTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGtt                                                                                                                                     >  2:334148/1‑81 (MQ=255)
                                                                              gTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGtt                                                                                                                                     <  1:334148/81‑1 (MQ=255)
                                                                              gTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGAc                                                                 >  2:51989/1‑149 (MQ=255)
                                                                               ttAGTGCAGGAGGGGGAAGAGGGTGGGTGCAAAAGGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACGGGTTATGGGGTTCGGTGCCTGACa                                                                <  2:71161/149‑1 (MQ=255)
                                                                                 aTTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTGATGGAGTTCGGTGCCTGACAgc                                                              >  1:328731/1‑148 (MQ=255)
                                                                                     cagcagGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCCAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGa                                                          >  2:330675/1‑149 (MQ=255)
                                                                                     cagcagGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGTGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGa                                                          >  1:305390/1‑149 (MQ=255)
                                                                                       gcagGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGAcc                                                        >  2:477678/1‑149 (MQ=255)
                                                                                        cagGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGATGACCAGTGCGCATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACcg                                                       >  1:475199/1‑149 (MQ=255)
                                                                                           gcgcAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCt                                                            <  1:548758/141‑1 (MQ=255)
                                                                                           gcgcAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCt                                                            >  2:548758/1‑141 (MQ=255)
                                                                                           gcgcAACAAGCTGGTGTCAAAACGATTTACAGAGATGCCAGCTACTGGTGTCAAGAGGCTGAACAGTTCACATTATGGAATGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGTTTCGGTGCCTGACAGGCTGACCGCGt                                                    <  2:224065/149‑1 (MQ=255)
                                                                                            gtcaacaaGGTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGTGGTTCGGTGCCTGACAGGCTGACCGCGTg                                                   <  1:29108/147‑1 (MQ=255)
                                                                                             gcAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGc                                                  >  1:477303/1‑149 (MQ=255)
                                                                                               aacaaGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAATCCTACCGCGTGCAg                                                <  2:663409/149‑1 (MQ=255)
                                                                                                 caaGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCGCGAAGCGGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACACGCTGACCGCGTGCGGag                                              >  2:448773/1‑148 (MQ=255)
                                                                                                  aaGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGaaa                                             >  2:953977/1‑149 (MQ=255)
                                                                                                    ggtGGGTGAAAAACCATTTATGGATACGGCATGTTGTTGTGGCAAGAGGTGGAACAGTTCACATTATGGACTGGCAAAGAGTTCCCTCTGTAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAgg                                           <  2:569507/147‑1 (MQ=255)
                                                                                                    gCTGGTTGCAAAACGATTGAGGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAgg                                           <  2:888751/149‑1 (MQ=255)
                                                                                                        gTTGGAAAACGATTGATGGATACGACAAGTTGGTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGTATATGTCAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGCGCGCGTGCAGAAAGGGTaa                                       >  2:811603/1‑149 (MQ=255)
                                                                                                           gCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTaaaaa                                    >  2:828157/1‑149 (MQ=255)
                                                                                                             aaaaCGGTTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACAGTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAAt                                  <  1:780410/149‑1 (MQ=255)
                                                                                                             aaaaCGATTGATGGATACGGCATGTTGTTGTTGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCAGGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAAt                                  <  1:282152/149‑1 (MQ=255)
                                                                                                             aaaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTCAGAAAt                                  >  1:395638/1‑149 (MQ=255)
                                                                                                             aaaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAAt                                  >  1:729706/1‑149 (MQ=255)
                                                                                                             aaaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCCGACCGCGTGCCGAACGGGTAAAAAAt                                  >  2:553794/1‑149 (MQ=255)
                                                                                                             aaaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGATCAAATTATGGACTGACAAGGATTTACCTATGGAACTTGTTAACATGCTTATGGAGTTCGGTGCATGACCTGCTGATCGCGTGCCGAAAGGGTAAAAAAt                                  >  2:93884/1‑149 (MQ=255)
                                                                                                              aaaCGATTGATGGATACGGCATGTTGTTGTAGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGGTGAACATGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATg                                 <  1:362357/149‑1 (MQ=255)
                                                                                                               aaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGATACTGGTGATATTGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGa                                >  1:518678/1‑149 (MQ=255)
                                                                                                                 cGATTGATTGATACGGCATGTTGTTTTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGaaa                              <  1:863069/149‑1 (MQ=255)
                                                                                                                  gATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGaaaa                             <  2:729706/149‑1 (MQ=255)
                                                                                                                        tGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTaa                       <  2:421206/149‑1 (MQ=255)
                                                                                                                            tACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGACATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAGATGAAAACCGTCACtat                   >  2:80673/1‑147 (MQ=255)
                                                                                                                            tACGGCATGTTATTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGt                   >  1:671664/1‑149 (MQ=255)
                                                                                                                                gCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATggg                                                                               <  2:405592/85‑1 (MQ=255)
                                                                                                                                gCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATggg                                                                               >  1:405592/1‑85 (MQ=255)
                                                                                                                                   tgttggtgtGGCAAGGGGCGGACTAGTGCAGCTTAAGGACAGGCAAAGATCTCCCTCAGGAAGATGATAAACAGTGAAAGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCCGCTAGGGTAAAAAATGAAACCCGTATCTGTAAAAGAt            <  2:901601/149‑1 (MQ=255)
                                                                                                                                    gtgtttgtGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGAtc           <  2:475199/145‑1 (MQ=255)
                                                                                                                                     ttgttgtGTAAAGAGGCTGAACAGATCACATTATGGACTGGCAAAGATTGCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGAtct          <  2:171791/149‑1 (MQ=255)
                                                                                                                                          gtgGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCa     <  1:280201/149‑1 (MQ=255)
                                                                                                                                             gCAAGAGGCTGAACATTTCACATTATTGACTGGCAAAGATTTCCCTCTGTAATATGTTAAACAGGGCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTg  <  2:992996/149‑1 (MQ=255)
                                                                                                                                             gCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTg  >  1:665594/1‑149 (MQ=255)
                                                                                                                                             gCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTg  <  1:51989/149‑1 (MQ=255)
                                                                                                                                             gCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTAGAATATGTTAAACAAGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTg  >  1:933171/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTG  >  NC_000913_3_hsa_tpiA/1774430‑1774719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑