Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,126,168	C→G	A209P (GCA→CCA)	priA ←	Primosome factor n' (replication factor Y)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,126,168	0	C	G	100.0%	127.0 / NA	38	A209P (GCA→CCA)	priA	Primosome factor n' (replication factor Y)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/18);  total (20/18)

CGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCG  >  NC_000913_3_hsa_tpiA/4126027‑4126293                                                                                                                                              |                                                                                                                              cGATTTCCGGCACCATGACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAAcg                                                                                                                        >  1:627675/1‑149 (MQ=255)  gATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAAcgg                                                                                                                       <  1:303863/149‑1 (MQ=255)                 cACCAGCGCGTGTTTACCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCgg                                                                                                        <  2:131473/149‑1 (MQ=255)                    catcgcCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCCATGAATTGGGCCAACGGCGGTGGCCTCTTCTGTaa                                                                                                     <  2:629219/146‑2 (MQ=255)                    caccgcCTGTATGCCCTGATCGAGCACGGTTTCCAGCACGCCCCGAAAATCCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTAt                                                                                                     <  1:316193/146‑1 (MQ=255)                    cAGCGCCTGGTGGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTAt                                                                                                     <  2:273289/149‑1 (MQ=255)                      gcgcCTGTTGGCCCTGCGCGAGCATGTTTTCCAGTACGCTGAGATAAGCCTCCGATTTACCCGAACCGGGAACGCCCGCCAGCAGCCCGCCAGAAACAGTATGTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTAttg                                                                                                   <  2:546425/149‑2 (MQ=255)                         ccTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGCATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAAt                                                                                                >  2:478555/1‑149 (MQ=255)                          cTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCACCGGTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATc                                                                                               <  2:272146/149‑1 (MQ=255)                          cTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCGGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATc                                                                                               >  2:629280/1‑149 (MQ=255)                             tttGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTCACGCCCGCCAGCAGCCAGGCAGAAAACGTATCTGCCGCGCTATGAGTTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCa                                                                                            >  2:477670/1‑149 (MQ=255)                                  ccTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGcc                                                                                                               <  1:647585/125‑1 (MQ=255)                                  ccTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGcc                                                                                                               >  2:647585/1‑125 (MQ=255)                                   cTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAACGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCCATCGCAACCCCt                                                                                      >  1:246565/1‑149 (MQ=255)                                             aCGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAAc                                                                            >  2:595697/1‑149 (MQ=255)                                              cGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACg                                                                           >  1:425712/1‑149 (MQ=255)                                                           gCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCGATCGCAACCGCTCACCAGAAACGGCATGgttcgttc                                                              >  2:624198/1‑149 (MQ=255)                                                           gCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgttcgttc                                                              >  1:87846/1‑149 (MQ=255)                                                           gCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGCGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAACCGGCACActtcgttc                                                              >  2:581781/1‑149 (MQ=255)                                                            cTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGttcgttcg                                                             <  1:462815/149‑1 (MQ=255)                                                             tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGc                                                            >  1:489251/1‑149 (MQ=255)                                                             tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACCGCATATTTCGTTCGc                                                            >  2:223914/1‑149 (MQ=255)                                                             tGAGATAAACCGCCGTTTTCCCGGCCCCGGTAACTCCCTCACGAAGACGATGAGAAAAAGTATCTGCAGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGc                                                            <  1:346221/149‑1 (MQ=255)                                                                     aCCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCCGAACAAATATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCACTCGCAACCGCTCACCGGCACCGGCGTAGTTCCTTCGCCAGTctct                                                    >  2:202771/1‑149 (MQ=255)                                                                     aCCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAggccct                                                    >  2:279062/1‑144 (MQ=255)                                                                     aCCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCt                                                    >  1:445468/1‑149 (MQ=255)                                                                        tCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTaaa                                                 >  2:589113/1‑149 (MQ=255)                                                                        tCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTaaa                                                 <  2:87846/149‑1 (MQ=255)                                                                              ttACCGGAACCGGCAACGCCCGCCATCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCACAGAGCGTTAGATTGTTCTTGTGTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTgg                                           <  1:707547/149‑1 (MQ=255)                                                                                   ggAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGttt                                      <  1:670510/149‑1 (MQ=255)                                                                                          gTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTGGCCAGTCGCTAAACTCTGGTGTTTCACTTGc                               >  1:157727/1‑149 (MQ=255)                                                                                                  cgccAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATcac                       <  2:237870/149‑1 (MQ=255)                                                                                                   gccAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTACTTCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATcaca                      <  1:710489/149‑1 (MQ=255)                                                                                                   gccAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCAACAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATcaca                      <  2:627675/149‑1 (MQ=255)                                                                                                    ccAGAAGGCAGGAAGAAAGATTAGCTGCCGTTGTATGATTTGGGCTAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATcacac                     <  2:398396/149‑1 (MQ=255)                                                                                                               gCAGAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCtttttt          >  2:373469/1‑149 (MQ=255)                                                                                                                  gAAAAAGTATCTGCCGCGCTATGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCg       >  1:96039/1‑149 (MQ=255)                                                                                                                       aggaTCTGCCGCGCTAGGAATTGGGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAgcg  <  2:90918/146‑1 (MQ=255)                                                                                                                                              |                                                                                                                              CGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCG  >  NC_000913_3_hsa_tpiA/4126027‑4126293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑