Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,159,168	G→A	A233T (GCA→ACA)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,159,168	0	G	A	96.8%	95.8 / NA	31	A233T (GCA→ACA)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (15/15);  minor base T (0/1);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTG  >  NC_000913_3_hsa_tpiA/4159030‑4159305                                                                                                                                           |                                                                                                                                          gCAGGGGAAAAACTGCTGATGCTGCAAGGTGGTCACTGTTTGCGAGAACAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAGCTCTGCGCAACATGTTGACGACAGGTGGCgg                                                                                                                                 >  2:83859/1‑149 (MQ=255)    gggtAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAGGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGAGCAGCCTGGAAACTCTGCGCAACATGGTGGCGTCAGGTAGCGGTAt                                                                                                                              <  2:87985/149‑1 (MQ=255)    ggggAAAAACTGCTGATGCTGGAAGCGGGTCACTGTTTGCGCGATCAGACAATGGGTTTCTGGTTTGAAGCCGGGGCGGATGAAGATACACACGTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGAt                                                                                                                              <  2:352135/149‑1 (MQ=255)       gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCgg                                                                                                                                 <  1:248047/143‑1 (MQ=255)       gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCgg                                                                                                                                 >  2:248047/1‑143 (MQ=255)            aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGGTCACTTTAc                                                                                                                      >  1:573210/1‑149 (MQ=255)             ctgccgATGCTGCAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAACCCGGGGCGGATGAAGATACACACTGCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACt                                                                                                                     <  1:154521/149‑1 (MQ=255)                 tgATGCTGGAAGATTGTCACTGTTTGCGCGATCAGTCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCa                                                                                                                 <  2:175695/149‑1 (MQ=255)                       tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTAc                                                                                                                      <  1:219163/138‑1 (MQ=255)                       tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTAc                                                                                                                      >  2:219163/1‑138 (MQ=255)                        ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCgctgg                                                                                                          <  2:377579/149‑1 (MQ=255)                          aaGATGGTCACTGTTTGCGCGACCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACCTGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCgctggct                                                                                                        <  1:643537/149‑1 (MQ=255)                            gATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt                                                                                                      >  1:405345/1‑149 (MQ=255)                              tGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgc                                                                                                    >  1:410565/1‑149 (MQ=255)                                    cTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGACGGGGCGGATGACGATACACACTTCCGCGCGACCAGCCTGGACACTCTGCGCAACATGGAGGCGACAGCGCGCGGGAGCACTTTACTGCCAGCGCTGGCTGTGccgccgt                                                                                              >  2:209284/1‑148 (MQ=255)                                                 cAGGCAATGGTTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGa                                                                                 <  1:363616/149‑1 (MQ=255)                                                     gaaTGGGTTTCTGTTTTGCCACTGCGTCGGAGGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATggg                                                                             <  1:491484/148‑1 (MQ=255)                                                            tttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGttt                                                                      >  1:196710/1‑149 (MQ=255)                                                              tCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                    >  1:301358/1‑149 (MQ=255)                                                                   tttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc                                                               <  1:717593/149‑1 (MQ=255)                                                                         gCCGGGGCGGATGAAGTTACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAt                                                         >  1:628856/1‑149 (MQ=255)                                                                             gggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg                                                     <  1:514980/149‑1 (MQ=255)                                                                             gggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg                                                     >  1:362716/1‑149 (MQ=255)                                                                             gggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg                                                     >  2:514980/1‑149 (MQ=255)                                                                                      aaGATACACCCCTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCa                                            <  1:151596/149‑1 (MQ=255)                                                                                             acacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCa                                     >  2:399251/1‑149 (MQ=255)                                                                                                          aCCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGACGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGt                        <  2:256189/149‑1 (MQ=255)                                                                                                              gCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAGCCGGATCACTTTACTGCCAGCGCTGGCTGTTCCGCAGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCCACCCGCCCTCTTGGCCTGGTTTAt                    >  2:82733/1‑149 (MQ=255)                                                                                                                            cgcAACATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCAcc      >  2:208203/1‑149 (MQ=255)                                                                                                                               aaCATGGTGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCt   <  1:305718/149‑1 (MQ=255)                                                                                                                                aCAGGGGGGCGACAGGTAGCGGGATCACTTTACTGCCAGCGCTAGGTATGCCGGGGTAGCTCAAACGCGATGGGGTTGTCCATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGTCCTGGTTTATCGTCCTGGCTCACCGCTg  <  2:582312/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                          GCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTG  >  NC_000913_3_hsa_tpiA/4159030‑4159305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑