Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	482,230	0	G	A	100.0%	267.9 / NA	81	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/38);  total (43/38)

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_hsa_tpiA/482084‑482375                                                                                                                                                    |                                                                                                                                                  tgccatAAACTTTCGTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCagg                                                                                                                                                 <  2:1164258/146‑1 (MQ=255)   acatAAACTTTCCTCAGTCGACAGTGGTCGATACAGGCGTTCACATAGCTGCGGCCCCATGCAGCGCGCAGGGTTGTGTTAATGTCGTTGATAGAATCACCCAGCGCGTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGt                                                                                                                                               <  2:909064/149‑1 (MQ=255)     atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                             >  1:1132594/1‑149 (MQ=255)         aCTTTCTTCACACGCCCGCGGTCGATAAAGTCGTTCACATAGCCGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCCG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                         <  1:204588/149‑1 (MQ=255)         aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                         <  1:1027325/149‑1 (MQ=255)          cTTTCTTCACACGCCCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTcc                                                                                                                                        <  1:618233/149‑1 (MQ=255)          cTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTcc                                                                                                                                        >  1:1191850/1‑149 (MQ=255)           tttCTTCACACGACCGCGGTCGATACAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGGCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                       <  1:164710/149‑1 (MQ=255)           tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                       >  1:491941/1‑149 (MQ=255)             tctgcACGCGACCGCGGCCGATAAAGTCGTTCACATCGCTGGCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGCCGTTGATAGAAACTCCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGa                                                                                                                                     <  1:270479/149‑1 (MQ=255)               ttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTACTGCCGTTGAATGAAACACCCTGCGCCTGCGCTTTTTCCTG‑GTCAATATCAATCTTAAACTGCAGGGTTTCTTccgcacc                                                                                                                                   >  2:138418/1‑144 (MQ=255)               ttcACACGACCGCGGTCGATAAAGTCGATCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGAcc                                                                                                                                   >  2:703502/1‑149 (MQ=255)                  acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                                <  1:1016701/149‑1 (MQ=255)                  acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                                >  1:276246/1‑149 (MQ=255)                   cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCCGAGTTGATTCAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                               <  1:604368/149‑1 (MQ=255)                   cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                               <  1:754555/149‑1 (MQ=255)                   cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGAAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                               <  1:789844/149‑1 (MQ=255)                   cacGACCGCGGTCGATAAAGTCGTTCACATAGCTACCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                               <  1:474701/149‑1 (MQ=255)                             gTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGc                                                                                                                     >  2:752720/1‑149 (MQ=255)                               cGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTg                                                                                                                   >  2:654842/1‑149 (MQ=255)                               cGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTg                                                                                                                   <  2:193445/149‑1 (MQ=255)                                      gTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                             <  1:491717/148‑1 (MQ=255)                                      gTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                             >  2:491717/1‑148 (MQ=255)                                         gTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACatat                                                                                                         <  1:934214/149‑1 (MQ=255)                                         gTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAAATGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACatat                                                                                                         >  2:1000688/1‑149 (MQ=255)                                          ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATc                                                                                                        >  2:1010352/1‑149 (MQ=255)                                           tCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCa                                                                                                       <  1:296143/149‑1 (MQ=255)                                            cacaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTCTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGAACGTACGCTGGTCAACATATcgg                                                                                                      >  2:867400/1‑147 (MQ=255)                                             acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                     >  1:330074/1‑149 (MQ=255)                                               aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                         >  1:1055506/1‑111 (MQ=255)                                               aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                         <  2:1055506/111‑1 (MQ=255)                                               aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                   >  1:771899/1‑149 (MQ=255)                                                    tGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATCTCAGGGTGCTTc                                                                                              >  1:400150/1‑149 (MQ=255)                                                           ccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACAACCCGGTCCGGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTAACTTCAAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGATTCtt                                                                                       >  2:944016/1‑149 (MQ=255)                                                            cATGCAGCTCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTc                                                                                      <  1:1234977/149‑1 (MQ=255)                                                            cATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATAAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTc                                                                                      >  1:542269/1‑149 (MQ=255)                                                                 aGCGCCCCGAGTGGTGTTAATGGCGTTGATAGCAACACGCAGCGCCTGCGCTGTTTCCTG‑GTCGATATCAATCTTAAACCGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                                 <  1:733590/149‑1 (MQ=255)                                                                     cccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCACGGTGCTTCGCTGCTTCTgcaagcaa                                                                             >  2:615882/1‑149 (MQ=255)                                                                      ccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                   >  2:751312/1‑126 (MQ=255)                                                                      ccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                   <  1:751312/126‑1 (MQ=255)                                                                       cAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACt                                                                           >  1:200666/1‑149 (MQ=255)                                                                       cAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACt                                                                           <  1:434572/149‑1 (MQ=255)                                                                               tgtTAATGTCGTTGATAAAAACCCCCGGCGCCTGCGCTTTCTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTCTGGACGAACGCTGGTCAACATATCAGGCTGCTTCGCTGCTCCTGCAAGCAACTGGCTAcgc                                                                   >  2:948913/1‑149 (MQ=255)                                                                                 ttAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACgcgc                                                                 >  1:731109/1‑149 (MQ=255)                                                                                  tAATGTCGTTTATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGcc                                                                <  2:905302/149‑1 (MQ=255)                                                                                     tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTAAAGAGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCGGCAAGCAACTGGTTACGCGCCTGa                                                             >  1:421288/1‑149 (MQ=255)                                                                                     tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACCGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                             <  1:205799/149‑1 (MQ=255)                                                                                     tGTCGCTTATAGAAACACGGAGGCGCTGCGCTTTTTCCGG‑GGCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTCGCCGTACCATGGTCAACATATCAGGGGGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                             <  2:521073/149‑1 (MQ=255)                                                                                         gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGCCa                                                         >  2:705765/1‑149 (MQ=255)                                                                                          ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAg                                                        <  1:1010352/149‑1 (MQ=255)                                                                                          gtgcaaGAAACCCCACGCGCGTGCGATTTCTCCTT‑GTCGATATCAATCTTAAACTGTAGGGTATCTTCCAGACCGTTTGCACGTACGCTGGTCAAAATATCAGGGGCCCTCGCGGCTTCTGCAAGGAAATGGTAACGCGCCTGAGTcgg                                                        <  2:1021910/144‑3 (MQ=255)                                                                                             aTAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGATTGCGCGCTGGAGTCGGttt                                                     >  2:508973/1‑149 (MQ=255)                                                                                                gAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                  >  1:42908/1‑149 (MQ=255)                                                                                                gAAACACCCACCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                  >  1:1210571/1‑149 (MQ=255)                                                                                                 aaaCACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCCGTTTTTCg                                                 >  2:1034561/1‑149 (MQ=255)                                                                                                    cacCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa                                              <  2:421288/149‑1 (MQ=255)                                                                                                      cccAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                            <  2:1083524/149‑1 (MQ=255)                                                                                                        cAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGGATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTCCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGCCAGTTTTTCGTGACCaa                                          >  2:53599/1‑149 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                         <  1:322985/149‑1 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                         <  1:741660/149‑1 (MQ=255)                                                                                                          gcgcCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTCCGCGCCTGAGTCAGTTTTTCGTGACCAAgg                                        >  1:489479/1‑149 (MQ=255)                                                                                                           cgcCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                       <  2:330074/149‑1 (MQ=255)                                                                                                               tgagcTTTTTCCTGCGTC‑ATATCAATCTTAAACTGCAGGGTATCCTCCAGACCGTTGGGACGTACGCTGGTCAACACATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc                                   <  2:22833/146‑1 (MQ=255)                                                                                                               tGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc                                   <  2:1191850/149‑1 (MQ=255)                                                                                                               tGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATATTCTAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAATCAGTTTTTCGTGACCAAGGCCAga                                   >  2:831512/1‑148 (MQ=255)                                                                                                                 cgcTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt                                 <  2:42908/149‑1 (MQ=255)                                                                                                                  ccTTTTTCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                                >  2:1233958/2‑149 (MQ=255)                                                                                                                    tttttCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                              >  1:507150/1‑149 (MQ=255)                                                                                                                    tttttCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAATCAGTTTTTCGTGCCCAAGGCCAGCCTgct                              >  2:442104/1‑147 (MQ=255)                                                                                                                        tCCTG‑GTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                          <  1:73267/149‑1 (MQ=255)                                                                                                                            tgttCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGTCCAAGGCCAGCCTGGTCAATCAg                       <  1:562619/146‑1 (MQ=255)                                                                                                                               tCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc                    <  2:507150/149‑1 (MQ=255)                                                                                                                                    atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                         <  1:220099/123‑1 (MQ=255)                                                                                                                                    atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                         >  2:220099/1‑123 (MQ=255)                                                                                                                                    atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                       <  1:754557/125‑1 (MQ=255)                                                                                                                                    atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                       >  2:754557/1‑125 (MQ=255)                                                                                                                                       aaTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa            >  1:13970/1‑149 (MQ=255)                                                                                                                                         tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag          >  1:473859/1‑149 (MQ=255)                                                                                                                                           ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc        >  1:425393/1‑149 (MQ=255)                                                                                                                                              aaCTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt     >  2:469419/1‑149 (MQ=255)                                                                                                                                                 tGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                                 >  1:540765/1‑70 (MQ=255)                                                                                                                                                 tGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc  >  1:242238/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTG‑GTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_hsa_tpiA/482084‑482375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑