Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,106,741	A→T	S162S (TCT→TCA)	wbbH ←	O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,106,741	0	A	T	98.8%	271.9 / ‑5.2	82	S162S (TCT→TCA)	wbbH	O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (41/40);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

ACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_hsa_tpiA/2106595‑2106886                                                                                                                                                    |                                                                                                                                                  aCTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGa                                                                                                                                                 >  1:470393/1‑149 (MQ=255)     tATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGGATTCAGTATAATTGCAAAGATGAATACACACAGTACAATTAAAGTAAATGTTTTACTTACACTTGTATTAGTA‑AATTTTTTTGAACaa                                                                                                                                             >  1:602673/1‑149 (MQ=255)      aTTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCaaa                                                                                                                                            <  1:1083141/149‑1 (MQ=255)         aaCACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTCGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCaaataa                                                                                                                                         >  1:1013950/1‑149 (MQ=255)             cgtACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAAGTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAataaataa                                                                                                                                     <  2:27697/147‑1 (MQ=255)              cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATTAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAAtaaataaa                                                                                                                                    >  1:578752/1‑149 (MQ=255)              cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAAtaaataaa                                                                                                                                    <  2:475805/149‑1 (MQ=255)              cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAAtaaataaa                                                                                                                                    >  2:375674/1‑149 (MQ=255)               tACGATGAATGCATAAGTGATGATAACAATAACCACAATGCGCTTCCCAGTATTCATTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGCCCAaatacatatat                                                                                                                                   >  2:268897/1‑146 (MQ=255)               tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGAATAATTGCAAAGATGAATACAATAAAAACAAGTCAAAACATTGTGTTACTTACTTTTGTATTAGTA‑AATTTTTTTGACCAAGAAAATAAAg                                                                                                                                   >  1:1099188/1‑149 (MQ=255)               aaCGATGAATGCATAAGAGATGAAAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAg                                                                                                                                   <  2:657263/148‑1 (MQ=255)                    tGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCaaaa                                                                                                                              <  1:207149/149‑1 (MQ=255)                     gAATGCATAAGAGACGATAACCATAAAGACAATTTACTTACCAGTATTCAGTATACTTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCATCGTATTCGTA‑ATTTTTTTTGACAACATAAGTAAAGCaaact                                                                                                                             >  2:617625/1‑147 (MQ=255)                           atcagagATGATAACCATAAAGACAACTTGCTTACCAGTATTCAGTATAATTGCAAACATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑CATTTTTTTGACTAAATAAATAAACCAAAcgttgtaa                                                                                                                       >  2:539949/4‑147 (MQ=255)                            tAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACAAAATAAATAAAGCAAAAGAAGATAg                                                                                                                      >  2:241781/1‑149 (MQ=255)                             aaGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTCTACGTACCGTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGa                                                                                                                     >  1:912584/1‑149 (MQ=255)                              agagATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGaa                                                                                                                    <  2:116815/149‑1 (MQ=255)                              agaaaagatAAACAGAAAGACAATTTAATTACCAGTATTCAGTATAAATGAAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACGTACCTTTGTATTAGTA‑AATTTTTGTGACCGGATAAATAAAGCAAAAGTAGTTAGaa                                                                                                                    <  2:183035/143‑1 (MQ=255)                               gagaTGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAAt                                                                                                                   <  1:257109/149‑1 (MQ=255)                               gagaTGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAAt                                                                                                                   >  1:922167/1‑149 (MQ=255)                                  actatAACCAAAAAGACTAGTTGCTTACCAGTACTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAt                                                                                                                <  2:746967/146‑1 (MQ=255)                                   tcatAACCATAAACACAATTTACTTACCAGTAGGCAGAATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGAACCACTTTGTAGATC‑TAGGAATCAAGCAAAAGTAGTTAGAATGAtt                                                                                                               >  2:259516/3‑149 (MQ=255)                                     agaaCCATAAAGAAAATTAGTTTACGTGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGGAAAGTAAATGATTTACTTACCTTTGTATTAGTA‑AATTTTTTTGAGCCGATGAATAAAGCAAAAGTAGTTAGAATGATTgg                                                                                                             <  2:894198/147‑1 (MQ=255)                                      aaaCCATAAAGAAAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGc                                                                                                            <  2:101609/148‑1 (MQ=255)                                         ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTtgctgc                                                                                                         >  2:1240594/1‑149 (MQ=255)                                          cATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAAAATGATTGACTGCa                                                                                                        >  1:723311/1‑149 (MQ=255)                                           aTAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAt                                                                                                       <  1:1024156/149‑1 (MQ=255)                                                   aaTTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTg                                                                                               >  1:518246/1‑149 (MQ=255)                                                   aaTTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTg                                                                                               >  1:850072/1‑149 (MQ=255)                                                          ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAtt                                                                                        >  1:478507/1‑149 (MQ=255)                                                           tACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAttt                                                                                       <  1:355814/149‑1 (MQ=255)                                                           tACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAttt                                                                                       <  2:597612/149‑1 (MQ=255)                                                           tACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAttt                                                                                       <  1:980814/149‑1 (MQ=255)                                                             caaGTATTCAGGATAATTGCAAAGAGGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt                                                                                     <  2:94296/147‑1 (MQ=255)                                                              cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAACGTAAATGTTTTACTTACCTTTGTCTTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAATTCtt                                                                                    >  1:269473/1‑149 (MQ=255)                                                              cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAACGTAATTAGAATGATTGGCTGACTGTAGTCTAAGaattctttt                                                                                    >  2:140818/1‑142 (MQ=255)                                                              cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                                    >  1:645129/1‑149 (MQ=255)                                                              cAGTATTCAATATAATTGCAAAGATTAATACAATAAAAACAAGTAAATTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAATTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                                    <  2:872704/149‑1 (MQ=255)                                                               agtagtCAGTATAATTACAAAGATGAATACAATAAAAGCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑ATTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCttt                                                                                   <  2:1148477/149‑2 (MQ=255)                                                                 tATTCAGTATAATTGTAAAAATTAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:420207/149‑1 (MQ=255)                                                                 tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:902211/149‑1 (MQ=255)                                                                      aGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGCAGGATTGGCTGCATGTATGCTGAGAAATTTCTTGCTGTTTc                                                                            >  1:335426/1‑149 (MQ=255)                                                                             ttGCTAAGATGAATACAATAAAATCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑CATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACa                                                                     <  1:360627/149‑1 (MQ=255)                                                                             ttGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACa                                                                     >  2:935410/1‑149 (MQ=255)                                                                              tGCAAAGATGAATACAATAAAAAAAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACAt                                                                    <  2:243566/149‑1 (MQ=255)                                                                                cAAAGAAGAATACAAAAAAAACAAGGAAAGTAAATGTTTCACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGGTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                                  <  2:615705/149‑1 (MQ=255)                                                                                 aaaGATGAATACAATAAAAACAAGTAAAGTAACTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTTGCTGCATTTATGCTTATAAATTTCTTCATGTTTTTTCAACATCAg                                                                 >  2:610068/1‑149 (MQ=255)                                                                                  aaGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGc                                                                >  1:158690/1‑149 (MQ=255)                                                                                       gAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTTTTTTATTTAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTGGATGTGTCTTCAACTTCAGCAtctc                                                           >  2:86237/1‑149 (MQ=255)                                                                                       gAATAAAATAGAAAAAAGTAAAGTAAATGTTTTACTTACTGTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           <  2:1140447/149‑1 (MQ=255)                                                                                          tACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTATTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        <  1:375674/149‑1 (MQ=255)                                                                                          tACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        >  2:563072/1‑149 (MQ=255)                                                                                           aCAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATACTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTAt                                                       >  2:94474/1‑149 (MQ=255)                                                                                             aaTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCa                                                     >  2:1076116/1‑149 (MQ=255)                                                                                              aTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTTTTTTTTTTTTTAAGGCATAATTATATAGAATGATTGGCTGCTTGTTTGCTGAGAAAATTCTTGATGTGTCTTCAACATAAGCATCTCTTATCaa                                                    >  2:824313/1‑149 (MQ=255)                                                                                               tAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaaa                                                   >  1:524411/1‑149 (MQ=255)                                                                                                 aaaaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAAtt                                                 <  2:138627/149‑1 (MQ=255)                                                                                                         taaagtaaaTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAGTTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCTTCTAGc                                         >  2:594579/1‑149 (MQ=255)                                                                                                              taaaTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagt                                    >  2:488111/1‑149 (MQ=255)                                                                                                              taaaTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagt                                    >  1:734119/1‑149 (MQ=255)                                                                                                                 aTGTGTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagtaag                                 <  2:765890/149‑1 (MQ=255)                                                                                                                   gTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       <  2:987991/61‑1 (MQ=255)                                                                                                                   gTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       >  1:987991/1‑61 (MQ=255)                                                                                                                        acttacCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTcc                          <  2:602673/149‑1 (MQ=255)                                                                                                                          ttacCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 >  1:803343/1‑92 (MQ=255)                                                                                                                          ttacCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:803343/92‑1 (MQ=255)                                                                                                                            acCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGaa                      <  1:631733/149‑1 (MQ=255)                                                                                                                              cTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt                    >  2:552887/1‑149 (MQ=255)                                                                                                                              cTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt                    >  2:545614/1‑149 (MQ=255)                                                                                                                               tttGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                   <  2:461286/149‑1 (MQ=255)                                                                                                                               tttGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                   >  2:609459/1‑149 (MQ=255)                                                                                                                                ttGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg                  <  2:734119/149‑1 (MQ=255)                                                                                                                                 tGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGt                 <  1:994615/149‑1 (MQ=255)                                                                                                                                  gTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa                >  1:325105/1‑149 (MQ=255)                                                                                                                                  gTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa                <  1:820061/149‑1 (MQ=255)                                                                                                                                    aTTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGt              >  1:1152120/1‑149 (MQ=255)                                                                                                                                       aGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTTTGAGCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTAc           <  1:609459/149‑1 (MQ=255)                                                                                                                                        gTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACt          >  2:411176/1‑149 (MQ=255)                                                                                                                                         tA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACtt         <  1:1192156/149‑1 (MQ=255)                                                                                                                                          a‑aaTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTa        <  2:850072/149‑1 (MQ=255)                                                                                                                                             aTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAAc      <  1:1076116/149‑1 (MQ=255)                                                                                                                                                ttttTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTTTCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTc   <  1:814455/149‑1 (MQ=255)                                                                                                                                                 ttttGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCa  <  1:241781/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_hsa_tpiA/2106595‑2106886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑