Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503	1979278	776	61 [0]	[0] 61	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	50 (0.780)	42/262	0.7	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_hsa_tpiA/1978354‑1978650                                                                                                                                                     |                                                                                                                                                     tAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATG                                                                                                                                                      >  2:652514‑M1/1‑149 (MQ=255)     aaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtata                                                                                                                                                  >  1:1079243‑M1/1‑145 (MQ=255)       tGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTAAACGTAACTACAAGCACGTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTAATTAACATAAATGtataag                                                                                                                                                >  2:136706‑M1/1‑143 (MQ=255)                 cacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGCTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                                      <  1:652514‑M1/149‑17 (MQ=255)                       tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                                <  2:335244‑M1/149‑23 (MQ=255)                       tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                                >  1:633419‑M1/1‑127 (MQ=255)                        cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCACTTAAAAAACATAAATGtataagtcatacttttgttttgg                                                                                                                               <  1:900700‑M1/149‑24 (MQ=255)                         tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                                                                              >  1:730158‑M1/1‑125 (MQ=255)                          aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                                                                             <  2:504953‑M1/149‑26 (MQ=255)                                    cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                   >  1:1152220‑M1/1‑114 (MQ=255)                                       aaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtctttcaatctg                                                                                                                >  1:473712‑M1/1‑111 (MQ=255)                                                   tGTACTCGTCACTACACGCACATACAACGTAGGGGGGGTGCGATTTTCAAAAATGCGGGATGCAGATCACACAAAACGCGCAAGTACTTAACATAAAGGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttt                                                                                                    <  1:279322‑M1/149‑51 (MQ=255)                                                     tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                                  <  2:1159142‑M1/149‑53 (MQ=255)                                                       cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatcttttaaaaagtttttcgc                                                                                                >  2:223013‑M1/1‑95 (MQ=255)                                                        tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtactacttttgttttgggtgtatttcaatctgttaaaaagttttttgct                                                                                               >  2:975663‑M1/1‑94 (MQ=255)                                                              cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                         >  1:56693‑M1/1‑88 (MQ=255)                                                                   cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                                                                    <  2:730158‑M1/149‑67 (MQ=255)                                                                      acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                                 >  1:830662‑M1/1‑80 (MQ=255)                                                                         tACAACGGAGGGGGGCCTCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                              <  1:1059470‑M1/149‑73 (MQ=255)                                                                          aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgataggctcgcaagcaaaaat                                                                             >  2:305249‑M1/1‑76 (MQ=255)                                                                          aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACAATCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                                                                             >  1:174667‑M1/1‑76 (MQ=255)                                                                            aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTGCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                           >  1:338095‑M1/1‑74 (MQ=255)                                                                                 agagttgctgcgaTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                      <  2:1152220‑M1/143‑81 (MQ=255)                                                                                        cTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagtaataat                                                               >  2:411283‑M1/1‑62 (MQ=255)                                                                                        cTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                               >  1:822140‑M1/1‑62 (MQ=255)                                                                                         ggccgttttcaataATGGGGGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttttgtccagcgatcaagcaaaaatgaaacaggaataatc                                                              <  2:871522‑M1/144‑89 (MQ=255)                                                                                            gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaa                                                           >  2:241178‑M1/1‑58 (MQ=255)                                                                                            gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgccacgctagcaagcaaaaatgaaaaaggaataatagta                                                           >  1:866553‑M1/1‑58 (MQ=255)                                                                                                  cAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggga                                                     <  1:624747‑M1/149‑98 (MQ=255)                                                                                                    ataatGCGTGATGCAGATCACAAAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatg                                                   <  1:1125033‑M1/149‑100 (MQ=255)                                                                                                      aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgtt                                                 >  2:1189178‑M1/1‑48 (MQ=255)                                                                                                        tGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                               >  1:488102‑M1/1‑46 (MQ=255)                                                                                                        tGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                               >  2:884906‑M1/1‑46 (MQ=255)                                                                                                             gATGTAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                          <  1:541958‑M1/149‑109 (MQ=255)                                                                                                                      cacacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatg                                                        >  2:773884‑M1/1‑32 (MQ=255)                                                                                                                      cacacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatg                                                        <  1:773884‑M1/126‑95 (MQ=255)                                                                                                                       acacaAAACCCTCAATTACGTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                                <  1:158058‑M1/149‑119 (MQ=255)                                                                                                                       acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                                <  1:168596‑M1/149‑119 (MQ=255)                                                                                                                        cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat                                                         <  1:910647‑M1/123‑94 (MQ=255)                                                                                                                        cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat                                                         >  2:910647‑M1/1‑30 (MQ=255)                                                                                                                         acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcacgtaaaaatgaaacaggaataatcgaagtgggatgttgcgcacagtcaaaataact                              <  2:201713‑M1/149‑121 (MQ=255)                                                                                                                           aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                            >  2:1076231‑M1/1‑27 (MQ=255)                                                                                                                                  tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa                     >  1:831133‑M1/1‑20 (MQ=255)                                                                                                                                       tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc                <  2:415572‑M1/149‑135 (MQ=255)                                                                                                                                       tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc                <  2:473712‑M1/149‑135 (MQ=255)                                                                                                                                       tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc                <  2:488102‑M1/149‑135 (MQ=255)                                                                                                                                         cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtaaaaaattttttcgctacgctagcaagcaacaatgaaacaggaataatcgaaatgggatgttgcgaacagtcaaaataactcaccgtaaataaccat              >  2:509333‑M1/1‑13 (MQ=255)                                                                                                                                         cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatatgttaaaaagtgtttcgcaacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcat              >  2:509484‑M1/1‑13 (MQ=255)                                                                                                                                          ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                         >  1:423946‑M1/1‑12 (MQ=255)                                                                                                                                          ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                         <  2:423946‑M1/57‑46 (MQ=255)                                                                                                                                           tAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatct            <  1:1076231‑M1/149‑139 (MQ=255)                                                                                                                                             atataAATTtataagttatatttttgttttgggtttatttaaatctgttaaaaagtttttcgctacgctagcaagaaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc          <  2:230920‑M1/147‑141 (MQ=255)                                                                                                                                               aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta        >  2:193491‑M1/1‑7 (MQ=255)                                                                                                                                               aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta        <  1:193491‑M1/149‑143 (MQ=255)                                                                                                                                               aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                      >  2:100481‑M1/1‑7 (MQ=255)                                                                                                                                               aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                      <  1:100481‑M1/87‑81 (MQ=255)                                                                                                                                                 aaaTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctgta      >  2:436857‑M1/1‑5 (MQ=255)                                                                                                                                                   aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa    >  1:797689‑M1/1‑3 (MQ=255)                                                                                                                                                   aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa    >  1:789952‑M1/1‑3 (MQ=255)                                                                                                                                                    tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttagctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaat   >  1:1141233‑M1/1‑2 (MQ=255)                                                                                                                                                     gtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaata  >  1:20179‑M1/1‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_hsa_tpiA/1978354‑1978650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑