Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,755,533	C→A	intergenic (‑392/‑165)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,755,533	0	C	A	100.0%	155.1 / NA	46	intergenic (‑392/‑165)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/19);  total (27/19)

ATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAA  >  NC_000913_3_hsa_tpiA/1755390‑1755678                                                                                                                                                |                                                                                                                                                  aTTGTCTAACATGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACGCAAAAACAGGCACTAACGtt                                                                                                                                              <  1:180181/149‑1 (MQ=255)  gatcttAACAAGTTGTAAATTATTAGAAACGCAGTTTTTGTTTTCCTTTTGGATTAATTCCAGCGTATAATGCGCGCCAAATGACTCTTGAATGGTTTCAGCACTTTGAGTTATAGAAAAAAAAAAAAAAAAAACAGGCACTAACGTTg                                                                                                                                             <  2:777964/144‑1 (MQ=255)    gTCTAAAAAGTTGTATATTTTATAAAACGCTGTTTTTGTTTTCCTTTGGGCATAATTTAAGCGTATAATTCGCGCCAATATACTCTTGAAAGCTTTCAGCACTTTGGACTGTAAAACAAAAAAAAAAAAAAACAGGCACTAACGTTggg                                                                                                                                           <  2:530965/149‑1 (MQ=255)       tAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGTATTAATTTCAGCGTATAATACGCGCCAATTGACTCTTAAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTGAAAAACAGGCACTAACGTCGGGCTg                                                                                                                                        >  1:763399/1‑149 (MQ=255)       tAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTg                                                                                                                                        >  2:655721/1‑149 (MQ=255)       tAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTg                                                                                                                                        <  1:410608/149‑1 (MQ=255)        aaCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGAGTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTggagatc                                                                                                                                       >  1:777964/1‑144 (MQ=255)         aCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTgag                                                                                                                                      <  2:433000/149‑1 (MQ=255)                    atTTTTTGAAACGCTGTTTTTGTTTTCCTTTGGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGcaca                                                                                                                           <  1:401472/149‑1 (MQ=255)                                    tttttgttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCt                                                                                                           >  1:131315/1‑149 (MQ=255)                                          ttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCg                                                                                                     >  2:809894/1‑149 (MQ=255)                                          ttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGAACACATTCCTCTGCACGCTTTTTCg                                                                                                     >  2:620588/1‑149 (MQ=255)                                           tttCCTTTTGTATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGa                                                                                                    <  2:285158/149‑1 (MQ=255)                                            ttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGAt                                                                                                   <  2:665352/149‑1 (MQ=255)                                               ctcttGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTc                                                                                                <  1:108011/146‑1 (MQ=255)                                                ttttGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGAAGAACTCAACGACGCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCa                                                                                               >  1:118693/1‑149 (MQ=255)                                                 tttGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAc                                                                                              <  2:131315/149‑1 (MQ=255)                                                      aTTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTga                                                                                                                                       >  2:50691/1‑103 (MQ=255)                                                      aTTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTga                                                                                                                                       <  1:50691/103‑1 (MQ=255)                                                         aaTTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCtt                                                                                      >  1:249545/1‑149 (MQ=255)                                                                gCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGa                                                                               >  1:842818/1‑149 (MQ=255)                                                                  gTATAATGCGCGCCAATTGACTCTTGAATTGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAgg                                                                             <  2:273232/149‑1 (MQ=255)                                                                    ataATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGca                                                                           >  1:383715/1‑149 (MQ=255)                                                                        tGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTGCTCTGCACGCTTTTTCGATGTCACTTATCCTTAGAGCGAGGcaccac                                                                       >  2:546234/1‑149 (MQ=255)                                                                                  ttGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCCCCTATTCTTAGAGCGCGCCACCACTACTTTCGTa                                                             >  2:762309/1‑149 (MQ=255)                                                                                           gAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGACACCACCACTTTCGTAATACCGGAt                                                    >  2:448609/1‑149 (MQ=255)                                                                                              tGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCg                                                 <  1:655721/149‑1 (MQ=255)                                                                                                gTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCt                                               >  2:149035/1‑149 (MQ=255)                                                                                                  ttCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTAGGATGTGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTGGTAATACCGGAGTCGCttt                                             >  1:165508/1‑149 (MQ=255)                                                                                                       cACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGTTACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTGGCTTTCCGGc                                        >  1:456516/1‑149 (MQ=255)                                                                                                        aCTTTGGACTGTAGAACTCAACGACTCAAAAACAGTCACTAACATTGGGCAGAAACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCa                                       <  1:328402/149‑1 (MQ=255)                                                                                                          tttGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCCGt                                     >  2:154759/1‑149 (MQ=255)                                                                                                          tttGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGt                                     <  1:149035/149‑1 (MQ=255)                                                                                                          cttGGACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGt                                     <  1:346616/148‑1 (MQ=255)                                                                                                            tGGACTGTAGAACTCAACGACTCAAAAACCGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGGCACCTAGCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTgc                                   <  1:762309/149‑1 (MQ=255)                                                                                                              gACTGTGGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTgcgc                                 <  2:88580/149‑1 (MQ=255)                                                                                                              gACTGTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTCCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCCGATTCCCTTTCCGGCCGTgcgc                                 >  2:331879/1‑149 (MQ=255)                                                                                                                  gTAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAg                             >  1:243297/1‑149 (MQ=255)                                                                                                                   tAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGa                            >  1:333317/1‑149 (MQ=255)                                                                                                                   tAGAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTACTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGa                            >  1:43838/1‑149 (MQ=255)                                                                                                                     gAACTCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGaaa                          >  2:323965/1‑149 (MQ=255)                                                                                                                         tCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCaa                      <  2:383715/149‑1 (MQ=255)                                                                                                                         tCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAAAACGAGACACCACCACTTTCGTAATACCGCATTCGCTTTCCGGCAGTTCGCCCAGAACGCac                      >  2:612456/1‑148 (MQ=255)                                                                                                                           aaCGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGt                    >  1:267140/1‑149 (MQ=255)                                                                                                                                 tCAAAAACAGGCACTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCAATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCGCGCCGTTCGCCCAGACAGCACGTTTCTcc              >  2:403983/1‑149 (MQ=255)                                                                                                                                             aCTAACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa  >  2:563816/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                  ATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAA  >  NC_000913_3_hsa_tpiA/1755390‑1755678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑