New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 3600976 =43 (1.080)4 (0.110) 4/256 4.9 8.4% intergenic (‑193/+52) rpoH/ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
?NC_000913_3_hsa_tpiA 3601013 = 48 (1.320)intergenic (‑230/+15) rpoH/ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCG  >  NC_000913_3_hsa_tpiA/3600864‑3601160
                                                                                                                                                     |                                                                                                                                                   
aTTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAgtgt                                                                                                                                                     >  2:350282/1‑150 (MQ=255)
   cACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAGGAAGGTCTCTTTCCCTGCTACGGCACCCCTTGCAGGGAAATAGtata                                                                                                                                                  >  1:444381/1‑150 (MQ=255)
    aCTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATaa                                                                                                                                                 >  1:283738/1‑150 (MQ=255)
        ttttATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAACATTTGACATATAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTAAAacacc                                                                                                                                             >  2:20572/1‑149 (MQ=255)
        ttttATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACg                                                                                                                                             <  2:193526/150‑1 (MQ=255)
        ttttATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGCATAacccc                                                                                                                                             >  2:66108/1‑147 (MQ=255)
              cAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTtta                                                                                                                                       <  2:575857/150‑1 (MQ=255)
               aGACCGTGATTTGATCCACAAGTGCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCTGGAAGAGACAACGGTGTCTCTTTCCCTGCTACGTAACCCATTGCAGGGAAAGAGTATAACACGCTTttat                                                                                                                                      <  1:350282/150‑1 (MQ=255)
                 aCCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttatta                                                                                                                                    >  2:392144/1‑150 (MQ=255)
                    gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTACATGCTACGGAACCCATTGCAGGGAAAGAGTATAACACCCTTTTATTATTc                                                                                                                                 >  2:139546/1‑150 (MQ=255)
                                  aaGTTCACTGCAAGCTTGTGAATAAATTACGCACAACCTGTGATGTAGAGATGAAATACCGGGAAGCGCGTCCGATGTGTCTTTGATCGCTTGTTAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGc                                                                                                                   <  1:164446/150‑1 (MQ=255)
                                          tGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgt                                                                                                           >  2:384716/1‑150 (MQ=255)
                                             aaGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttgt                                                                                                        >  1:490906/1‑150 (MQ=255)
                                                cTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTAcc                                                                                                     >  1:554120/1‑150 (MQ=255)
                                                 ttGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCg                                                                                                    >  2:664717/1‑150 (MQ=255)
                                                      aaTAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                                                             <  2:369944/120‑1 (MQ=255)
                                                      aaTAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                                                             >  1:369944/1‑120 (MQ=255)
                                                            ttACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCAc                                                                                         <  2:283738/150‑1 (MQ=255)
                                                             tACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCATGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACg                                                                                        >  2:641970/1‑150 (MQ=255)
                                                                   caAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCa                                                                                  >  2:126478/1‑150 (MQ=255)
                                                                               gagagGTGGAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGTAACCCATTGCAGGGAAAGAGTATAACCCGCTTTTCTTATTCGGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatca                                                                      <  2:367179/149‑1 (MQ=255)
                                                                                 gagaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                                    <  1:126478/150‑1 (MQ=255)
                                                                                   gaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGa                                                                  <  1:392144/150‑1 (MQ=255)
                                                                                   gaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCAGCGCTGCCACCCAGCCAatcatcca                                                                  >  2:527127/1‑148 (MQ=255)
                                                                                   aacgAAATACCGGGAATAGACAACTGGGTCTCTTTCCCTGCTACGGAAACCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGTCAAGCTACGCTGCCACCCAGCCAATCATCgt                                                                  <  2:589709/147‑2 (MQ=255)
                                                                                         aTACCGGGAAGAGACAACGGGGTCTCTTCCCCTGCTACGGAACCCAGTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCAACCCAACCAATCATCGAGCATaa                                                            >  1:690806/1‑149 (MQ=255)
                                                                                          tACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCTTCGAGCATAcc                                                           <  1:440939/150‑1 (MQ=255)
                                                                                           aCCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACca                                                          <  2:444381/150‑1 (MQ=255)
                                                                                              gggAAGAGCACACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCCATCATCGAGCATACcagca                                                       <  1:317768/150‑1 (MQ=255)
                                                                                               ggAAGAGACAACGGGGTCTCTTTCCCTGCTACGAAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTGTTCAGGCGGAAAGTGGAGTATATGTTGTACCGTGCCAAGTCACGGTGCAGCCCAGCCCATCATCCAACATACtagaaa                                                      >  2:169103/1‑147 (MQ=255)
                                                                                                gAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                                    >  2:73115/1‑135 (MQ=255)
                                                                                                gAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                                    <  1:73115/135‑1 (MQ=255)
                                                                                                  agagACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCagaagcca                                                   <  1:664717/150‑3 (MQ=255)
                                                                                                      aCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGc                                               <  1:527127/150‑1 (MQ=255)
                                                                                                      aCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGc                                               >  1:588501/1‑150 (MQ=255)
                                                                                                       cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCATGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                                              <  1:641970/150‑1 (MQ=255)
                                                                                                               tctcTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCa                                      >  1:506518/1‑150 (MQ=255)
                                                                                                               tctcTTTCCCGGTTACGGAACCCATTGCAGGGAAAGAGTATAACACTCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCa                                      <  2:482422/150‑1 (MQ=255)
                                                                                                                     tCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                                <  2:554120/150‑1 (MQ=255)
                                                                                                                      cccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATg                               >  1:346799/1‑150 (MQ=255)
                                                                                                                        cTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGAt                             >  2:173879/1‑150 (MQ=255)
                                                                                                                         tGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCaa                                                                                              <  2:548502/84‑1 (MQ=255)
                                                                                                                         tGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCaa                                                                                              >  1:548502/1‑84 (MQ=255)
                                                                                                                           cTACGTAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAg                          <  2:317000/150‑1 (MQ=255)
                                                                                                                               ggAACCCATTGCAGGGAAAGGGTATAACACGCTTTTATTATTCAGGCGTAAAGTTGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCAt                      <  1:115538/150‑1 (MQ=255)
                                                                                                                                   cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGaa                                 <  1:602326/135‑1 (MQ=255)
                                                                                                                                   cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGaa                                 >  2:602326/1‑135 (MQ=255)
                                                                                                                                       ttGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAAGTTGTACCGTGGAAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAAGAGCAGGCATTCATCGAATGCTAAGCCATTGATGTCa              >  2:146843/1‑150 (MQ=255)
                                                                                                                                          cAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAAc           >  2:123139/1‑150 (MQ=255)
                                                                                                                                             ggAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTc        <  1:123139/150‑1 (MQ=255)
                                                                                                                                                   agTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCg  >  1:97668/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCG  >  NC_000913_3_hsa_tpiA/3600864‑3601160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.