New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 4347357	18 (0.730)	3 (0.130)	3/266	3.2	14.0%	intergenic (‑49/+29)	fumB/dcuB	anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
	?	NC_000913_3_hsa_tpiA	= 4347367	20 (0.870)					intergenic (‑59/+19)	fumB/dcuB	anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGG  >  NC_000913_3_hsa_tpiA/4347226‑4347501                                                                                                                                    |                                                                                                                                                 tAATCGGAAGTGGTTTGGTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCAc                                                                                                                                 <  2:283200/149‑1 (MQ=255)            gAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCCCGCAACGTGCAt                                                                                                                      >  2:320501/1‑149 (MQ=255)                  aTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATaa                                                                                                                <  1:364949/149‑1 (MQ=255)                  aTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATaa                                                                                                                <  2:149649/149‑1 (MQ=255)                  aTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGATTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGGGTGGTCACGGGCGGCACGCAAAGTGCAGTtatag                                                                                                                >  1:156608/1‑148 (MQ=255)                    aGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTCGTTATTTGTATTTGTCAGGCCGCGTAGTCACGGTCTGCGCGCAAAGTGCGTTTATAAGa                                                                                                              >  1:314173/1‑149 (MQ=255)                        ttCGGTATTGTCTTTCCCCATCGGGAAAGGGGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGGGCATTTATAAGAAccc                                                                                                          >  1:408543/1‑149 (MQ=255)                        tgccgTATTTTCTTTCCCCAGCGGGAAAGGTCCCTGGTAGATAAAGGTTTTTTTTGACATACCTTCCAGCCTTTAACTCTTTATTTGTTATTCGAAAATGGCGTACCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAAccc                                                                                                          <  1:245990/145‑1 (MQ=255)                                       ccccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCATGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGaa                                                                                           <  1:288792/149‑1 (MQ=255)                                                        ccTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCgcgg                                                                                               <  1:149381/128‑1 (MQ=255)                                                        ccTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCgcgg                                                                                               >  2:149381/1‑128 (MQ=255)                                                                            tttgACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGGAAATGGCGTGCCGCGTAGTAACGGGCGGCACGTATAGTGTAGTTATAAGACGGCGTAGACCGGGGGGAAGGACACGCCGAATACGAAAGATACGCTCGCCCCGATc                                                      >  2:285489/1‑149 (MQ=255)                                                                                    aGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCgg                                              >  1:5178/1‑149 (MQ=255)                                                                                                  aaCTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAGCCCGTACATCGCGGCGAAGATCCAGCCGGAGACGCACGCTACGCCCACCACACTCCCCCCCGTGAGAGTGAAGCtgt                                >  1:174461/1‑149 (MQ=255)                                                                                                         tATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTAGAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgat                         <  2:314173/149‑1 (MQ=255)                                                                                                              gTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGa                    >  1:150609/1‑149 (MQ=255)                                                                                                               ttATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGaa                   >  1:81071/1‑149 (MQ=255)                                                                                                                                gCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATgtgggtgg  <  2:221480/149‑1 (MQ=255)                                                                                                                                    |                                                                                                                                                 TAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGG  >  NC_000913_3_hsa_tpiA/4347226‑4347501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.