Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,864,7630AT22.7% 35.2 / 10.7 22intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (7/10);  new base T (2/3);  total (9/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913_3_hsa_tpiA/1864615‑1864910
                                                                                                                                                    |                                                                                                                                                   
ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                     <  2:24680/149‑1 (MQ=255)
ccGGATGAAGGCTACCAAACATTTGGTTGTGTAGAAACTGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                     <  2:98087/149‑1 (MQ=255)
   gatgatGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGATAATGTTGTGTGCCGGGTGCAATGc                                                                                                                                                  <  1:453510/149‑1 (MQ=255)
         gtcTACAAAAGATTTGTTTGTGTAGAAACGGCGTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACAGCTGGCGCAATCCATTCGGGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                            <  1:101147/147‑1 (MQ=255)
                    aTTTGCTTGTGTAGAAAGGGCTGACTACTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCATAACTGGCGCAATCCATTCGCGTTGCGAACCGTTAATTTACGTGAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                                 <  1:46108/149‑1 (MQ=255)
                                aGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                     <  1:286640/149‑1 (MQ=255)
                                aGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                     <  1:286638/149‑1 (MQ=255)
                                     cGGCTTACGCTTAAGAAACGCAAAAAGTGACCAAAGAGAATCCTTCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                                <  2:277991/149‑1 (MQ=255)
                                         ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttt                                                                                                            >  1:311568/1‑149 (MQ=255)
                                                    aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                 >  1:51650/1‑149 (MQ=255)
                                                                     aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                <  1:391618/149‑1 (MQ=255)
                                                                         agaAACCTGCACATCTGGCGCAATCCATTAGCGCTACAAAACGTAAATTTTCGTTAATGTTGTGTACCGGGTGCAATGCATCCGGCACACAACATCGAACCAAAACCAGCACAGATTTTGCTTTTGGTGCCAGATCTGCCTTATCCaca                                                                            >  2:108533/1‑147 (MQ=255)
                                                                                            gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                         >  2:445310/1‑149 (MQ=255)
                                                                                              aacccATTCGCCTTGCGAAACGGTAATTTGCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                       <  1:5190/146‑1 (MQ=255)
                                                                                                   aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                                  <  1:445310/149‑1 (MQ=255)
                                                                                                                   gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATAACCAGCGCAGTTTTTCCTTTTGGTTCCGGATATTCCTTATCCAGAATAGATAATTCTACCGATGAAAGTTCGACCTCGAGCCCAGca                                  >  2:1801/1‑148 (MQ=255)
                                                                                                                                 aTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGAc                    <  1:91739/149‑1 (MQ=255)
                                                                                                                                     tgtgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                <  2:477194/149‑1 (MQ=255)
                                                                                                                                       tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGAAATGGGc              >  1:255208/1‑149 (MQ=255)
                                                                                                                                        gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa             >  1:426444/1‑149 (MQ=255)
                                                                                                                                        gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                        <  1:272510/138‑1 (MQ=255)
                                                                                                                                        gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                        >  2:272510/1‑138 (MQ=255)
                                                                                                                                           ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                >  2:413164/1‑143 (MQ=255)
                                                                                                                                           ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                <  1:413164/143‑1 (MQ=255)
                                                                                                                                                   aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTATGTTAGGCATGGGCAATCGTGGCCGc  >  1:440432/1‑149 (MQ=255)
                                                                                                                                                   aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTATTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc  >  1:440437/1‑149 (MQ=255)
                                                                                                                                                    aTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                          >  2:251571/1‑60 (MQ=255)
                                                                                                                                                    aTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                          <  1:251571/60‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913_3_hsa_tpiA/1864615‑1864910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑