Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,304,4800GA21.4% 38.6 / 11.5 28intergenic (+87/+628)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (9/13);  new base A (3/3);  total (12/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTG  >  NC_000913_3_hsa_tpiA/2304334‑2304599
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gATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGTCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                                                                       <  1:252713/149‑1 (MQ=255)
gATGTGAAGTACCGCGGCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                                                                       <  2:442635/149‑1 (MQ=255)
  tgtgCAGTACCGCGGCTGGAAGGCGGGAGGGAGAATTGACAACGCGGTAGTGCGCTAAACTGCCGGGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGc                                                                                                                     >  2:371469/1‑149 (MQ=18)
     gAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                  <  2:130645/149‑1 (MQ=255)
            cgcgTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                           >  2:379875/1‑149 (MQ=255)
                  tGGAAGGCGGAAGAGAAAATTGACGACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCCACGATAGAGGCGTGGGTCTGATGCGACGCTGTCGCGTCTTATCAg                                                                                                     <  1:371469/149‑1 (MQ=18)
                    gAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                   >  1:216719/1‑149 (MQ=255)
                       ggCGGAAGAGAAGATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                <  2:49459/149‑1 (MQ=255)
                                          aaCGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                            >  1:211181/1‑118 (MQ=255)
                                          aaCGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                            <  2:211181/118‑1 (MQ=255)
                                          aaCGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCgcc                                                                             >  1:194894/1‑147 (MQ=255)
                                          aaCGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGc                                                                             >  1:355932/1‑149 (MQ=255)
                                                  aGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                     <  1:419327/149‑1 (MQ=255)
                                                   gtccgatAAACTGACGTGAAGCTCCGAGCCCAGGAGGGCAGACAAGGCGGACACGCCGCATCCGACATCCAACCCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGTTTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    <  2:104490/143‑1 (MQ=11)
                                                       gCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCAACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    <  2:322959/145‑1 (MQ=255)
                                                       gCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCAACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    <  2:73236/145‑1 (MQ=255)
                                                       gCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCAACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    <  2:73098/145‑1 (MQ=255)
                                                       gCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCAACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    >  1:73236/1‑145 (MQ=255)
                                                       gCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCAACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    >  1:73098/1‑145 (MQ=255)
                                                       gCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCAACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAg                                                                    >  1:322959/1‑145 (MQ=255)
                                                                  gTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCgc                                                                              >  2:265150/1‑123 (MQ=11)
                                                                  gTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGAGACGTTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGt                                                     <  2:350122/149‑1 (MQ=255)
                                                                                        tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCGCGCCGGAGATCAGAGAAGGCGGTTACGCCGCATCCGACGTccgcc                               >  2:490895/1‑146 (MQ=11)
                                                                                          aGACAAGGCGGTCACGCCGTATCGGAGACCAAACCCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTTACAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGc                             <  2:11662/149‑1 (MQ=17)
                                                                                            aCAAGGCGGTCAAGCCGCATCCGACAACCAACCCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGTCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGTCGGTCACGCCGCATCCGACATCCAACGccc                           <  1:101107/149‑1 (MQ=14)
                                                                                                    gTCACGCCGCATCCGACAGCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTCCGGCCCCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGt                   <  1:268593/149‑1 (MQ=255)
                                                                                                                ccGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGa       <  1:56599/149‑1 (MQ=255)
                                                                                                                     aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTACGGCCCCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGACCCGGTTGCCTGATGcgccgccg  >  1:121835/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                       
GATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTG  >  NC_000913_3_hsa_tpiA/2304334‑2304599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑