Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,197,502	C→T	G11R (GGA→AGA)	ymfD ←	e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,197,502	0	C	T	100.0%	70.7 / NA	22	G11R (GGA→AGA)	ymfD	e14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/14);  total (8/14)

ATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAG  >  NC_000913_3_hsa_tpiA/1197360‑1197647                                                                                                                                               |                                                                                                                                                   aTTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat                                                                                                                                              >  1:130542/1‑149 (MQ=255)     aTTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatta                                                                                                                                          <  1:393178/149‑1 (MQ=255)         atcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGt                                                                                                                                      <  2:286437/149‑1 (MQ=255)              aaTATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATGCTCGCAAAGATATCTAGAGGGCATCTTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa                                                                                                                                 <  2:207525/149‑1 (MQ=255)              aaTATCTAAGTCTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa                                                                                                                                 <  2:287870/149‑1 (MQ=255)                    tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGt                                                                                                                           <  1:274592/149‑1 (MQ=255)                                     aaaTCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTc                                                                                                                    >  1:177549/1‑139 (MQ=255)                                     aaaTCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTc                                                                                                                    <  2:177549/139‑1 (MQ=255)                                                                                  cAAAGATATCTATAGGTCATCGAATGAGGTTTTGCTACATTATCTGAGCGAATATTAATTCTGTGTATATTATCGTTCAAAGCAAGTACCATTCAATTAATGTAAA‑ATAAAATAAAAAATTCTTTTATTTTATCGTCCGGGATTTTTcc                                                            <  2:331865/149‑1 (MQ=255)                                                                                            tAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCgacttgactt                                                  >  1:57276/1‑149 (MQ=255)                                                                                                      cGTATGAGGTTTTGCTGCATTCTCTGAGCCAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTgg                                        <  1:192056/149‑1 (MQ=255)                                                                                                           gAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTa                                   >  2:275309/1‑149 (MQ=255)                                                                                                                tttGCTGCATTGTCGGAGCGAATATTAATTTTGTGCATATTATAGTTCAAAGTAAGTACCATTCAATTAATGTTAT‑TGTTAGTGAAAAATTCTTTTATTTTATCGTCTGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGt                              <  2:245485/149‑1 (MQ=255)                                                                                                                        aTTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaa                      <  1:450094/149‑1 (MQ=255)                                                                                                                         ttCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaaa                     <  2:57276/149‑1 (MQ=255)                                                                                                                          tctcTGAGCGAATATTAACTGTGTGCATATTATAGTTCAATGTGATTGCGATTAAATTATTGTTCTGCATTTGT‑AAAAATTCTTTTATTTTATCGTCCGTGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaaag                    >  2:356968/1‑149 (MQ=255)                                                                                                                              tGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGataa                <  1:275309/149‑1 (MQ=255)                                                                                                                                aGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGataata              >  1:26587/1‑149 (MQ=255)                                                                                                                                aGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATAAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATAATCCGGGATTTTTTCGACTTGACTTTGAGTTCTGGGCTTAACTGTCAAAGCAAAGataata              >  1:241780/1‑149 (MQ=255)                                                                                                                                   gAATATTAAATCTGTGCAACTTATAGTTCAAAGCAAGTAGCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATATTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTCAACTGTCAAAGCAAAGATAATACca           <  1:381909/149‑1 (MQ=255)                                                                                                                                     atatTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACcact         >  2:143373/1‑149 (MQ=255)                                                                                                                                          aaCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTg    <  1:107221/149‑1 (MQ=255)                                                                                                                                            ctctGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGGTTAACTGTCAAAGCAAAGATAATACCAGTCACagag  >  1:164809/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                   ATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAT‑TTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAG  >  NC_000913_3_hsa_tpiA/1197360‑1197647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑