| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_hsa_tpiA | 2,430,917 | C→G | intergenic (‑154/+105) | cvpA ← / ← dedD | colicin V production protein/membrane‑anchored periplasmic protein involved in septation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 2,430,917 | 0 | C | G | 93.3% | 83.9 / 0.3 | 30 | intergenic (‑154/+105) | cvpA/dedD | colicin V production protein/membrane‑anchored periplasmic protein involved in septation |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (14/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
CCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGC > NC_000913_3_hsa_tpiA/2430771‑2431059 | ccACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATggg < 1:569418/149‑1 (MQ=255) tACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCgt < 1:73691/149‑1 (MQ=255) gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTc > 1:341116/1‑149 (MQ=255) cTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGt > 2:515914/1‑149 (MQ=255) tGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTGCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCCGCGTa > 1:400730/1‑149 (MQ=255) gcgcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt > 2:433959/1‑149 (MQ=255) aCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa < 2:38972/149‑1 (MQ=255) agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATCCGTGTCGGATGCGCCGTAAACGCCTTATCCCACCTAcc > 2:414985/1‑148 (MQ=255) tttGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGATTCTACCCCTGCg > 1:407631/1‑149 (MQ=255) tGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTa < 1:439981/149‑1 (MQ=255) gCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAg < 1:363083/149‑1 (MQ=255) aGGGAGTGCCTTCCCTCGCATGAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTGTTATGCCTTTGCGGCATGGGGCATTGCGTGTCGGATGCGGCGTAAACGCCTTATTCGACCTACGGTTCTACCCCTGCGTAGGCCt < 2:585893/149‑1 (MQ=38) cttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGTGGCGTAAACGCCTTATCCGACCTACGGTTCCACCCCTCCGTAGGCCTCATAAGAcc > 2:167713/1‑148 (MQ=39) cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc < 1:262359/133‑1 (MQ=255) cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc > 2:262359/1‑133 (MQ=255) cATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGt < 2:575536/149‑1 (MQ=255) aaaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa < 2:317149/149‑1 (MQ=255) aaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg > 1:405616/1‑149 (MQ=255) gcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAc > 2:597253/1‑123 (MQ=255) gcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAc < 1:597253/123‑1 (MQ=255) gAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTa < 1:556545/149‑1 (MQ=255) aaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTaa < 2:545946/149‑1 (MQ=255) aaCGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcgc < 2:573414/110‑1 (MQ=255) aaCGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcgc > 1:573414/1‑110 (MQ=255) ccccATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCGGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGtat < 2:99716/147‑1 (MQ=255) tCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGccc > 1:191753/1‑149 (MQ=255) gACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGGGTATAGcccgt > 1:152845/1‑147 (MQ=255) aCTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGCCGCATCAGGCAAGACCGTATTAATTCGGCGTATACACCAtt > 1:301323/1‑149 (MQ=255) tttttATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGGCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAc > 2:549553/1‑149 (MQ=255) tttttAAGCCTTTCAGGGATGGGGCAATGCGTGTGGGATGGGGCGTAAACCCCTTCCCAGACCTACGGTACACACCCTGAGTATGCCCGATAAGACGCGCCCGGCTCGCTTGTGGCGGTTCGGGGTTAGTTCGGCGTATAGCCCATTAc < 2:120889/149‑1 (MQ=255) cGGCATGGGGCAATGCGTGCCGGAGGGGGCGTAAACGCCTCATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc < 2:156333/149‑1 (MQ=39) | CCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGC > NC_000913_3_hsa_tpiA/2430771‑2431059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |