Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,296,0420CT21.7% 112.2 / 23.0 69intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base T (11/4);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAG  >  NC_000913_3_hsa_tpiA/4295895‑4296186
                                                                                                                                                   |                                                                                                                                                
taggATAAGACGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                                                                                                                                                 >  2:316204/3‑149 (MQ=17)
  ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGGCATCAATGcc                                                                                                                                               >  1:74182/1‑149 (MQ=12)
    aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGCTGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGCATCAGACATCAATGCCTg                                                                                                                                             >  2:20498/1‑149 (MQ=11)
       aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGTTg                                                                                                                                          >  2:723265/1‑149 (MQ=17)
         gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                        >  2:168876/1‑149 (MQ=17)
            tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                                                                     <  1:168876/149‑1 (MQ=17)
              tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGCTTACGCCGCATCCGACCTCAACGCCTGATGCGACGcc                                                                                                                                   >  1:406816/1‑148 (MQ=12)
                tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                                                                 >  2:229729/1‑149 (MQ=17)
                tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                                                                 <  1:187385/149‑1 (MQ=32)
                 gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                                                                                                                <  1:316204/149‑1 (MQ=34)
                  ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATACACCATGCGACGCTTGcc                                                                                                                               >  2:274732/1‑149 (MQ=11)
                   cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAATCCCCGAACCGACGCTTGccc                                                                                                                              >  2:306560/1‑148 (MQ=11)
                   cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                                                              >  1:213344/1‑149 (MQ=25)
                      aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                           <  1:19041/149‑1 (MQ=21)
                        acggtaTCAGCGCCTGTGGCGACGTTTGACGGGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGTCGATCGGGTACGGAGTCTACGCCGCACCCGGCATCAACGCCTGAGGCGACGCTTGCCGCGTCt                                                                                                                         <  2:256333/144‑1 (MQ=11)
                           aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                      <  1:616704/149‑1 (MQ=21)
                           aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                      >  2:548157/1‑149 (MQ=21)
                            cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                     <  1:46560/149‑1 (MQ=39)
                                aaCGCCTGATGCGACGGTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                 >  1:644821/1‑149 (MQ=38)
                                aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGATCGGATAAGGCGTTTACGCCGCATCCGACATCCATACCTGATGCGACGCTTCCCCGCTCTTATCACGc                                                                                                                 >  2:720620/1‑149 (MQ=11)
                                 aCGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTAATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                >  1:652214/1‑149 (MQ=255)
                                    ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                             >  1:502953/1‑148 (MQ=18)
                                      tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc                                                                                                           >  1:352826/1‑149 (MQ=37)
                                            gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCAGAAATTTCCGTCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACgccagcca                                                                                                     >  1:544043/1‑149 (MQ=11)
                                            gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGTCTGATTTAGGGTGATTTTGTAGGTCGGGTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTACGTTAGACa                                                                                                     >  2:406500/1‑149 (MQ=11)
                                                      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                           <  1:131279/149‑1 (MQ=39)
                                                      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCCGACAGCGCataagc                                                                                           >  1:189856/1‑144 (MQ=255)
                                                           ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTAGTAGGTCGGATAAGACGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                                                                      >  1:648837/1‑149 (MQ=255)
                                                             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                     <  2:393883/132‑1 (MQ=40)
                                                             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                     >  1:393883/1‑132 (MQ=38)
                                                              tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCATCTTATCAGGCCTACGCCAGACGGCGCAGTGGccaggctaa                                                                                   >  2:239300/1‑142 (MQ=11)
                                                              gcAGGCTTAGGGCAGGGAGCGATATGGCATGATTTAGCGTGATTTTTTAAGGCGTGTCAGGCGTTTACGCCGCATACGCCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                                                                   <  2:193245/148‑1 (MQ=11)
                                                                aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                 >  1:89304/1‑117 (MQ=40)
                                                                  ggcTACGCGAGGGAGCGCATTAGCCTGATTTAGCGTGATTTTGTGGGTCGGGTAAGGGGTTTACGCCGCGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt                                                                               <  2:552405/147‑1 (MQ=11)
                                                                   ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATGCCAAACAGCGCAATAGTTTTCTTTAGCGTg                                                                              >  2:509942/1‑149 (MQ=11)
                                                                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGCTTTAGCGtca                                                                             >  2:544207/1‑147 (MQ=255)
                                                                        gcgaGATAGCGAGATAGCCTGATTTAGCGTGATTTTGTGGGGCGGTTAAGGTGTTTACGCCGCATCCGACATCAACGCCTGATGGGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                                                                         <  2:406816/146‑1 (MQ=11)
                                                                          cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtt                                                                                                                                  <  2:282233/90‑1 (MQ=35)
                                                                          cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                 <  1:113350/139‑1 (MQ=40)
                                                                          cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                 >  2:113350/1‑139 (MQ=40)
                                                                           aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                      <  2:51802/149‑1 (MQ=37)
                                                                               ggcgcATTGGCCTGATTTCGGGGGATTTGGTAGGTCGGATAGGGGGTTTCCGCCCCATCCGGCATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                  <  2:108592/148‑1 (MQ=11)
                                                                               aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                         >  2:264150/1‑126 (MQ=40)
                                                                               aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                         <  1:264150/126‑1 (MQ=40)
                                                                                    actgggcTGGTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                                             <  2:523700/143‑1 (MQ=255)
                                                                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                                             >  1:488328/1‑149 (MQ=38)
                                                                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc                                                         <  1:276315/149‑1 (MQ=38)
                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                   >  1:83938/1‑104 (MQ=18)
                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                           <  1:365578/64‑1 (MQ=255)
                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                           >  2:365578/1‑64 (MQ=255)
                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                   <  2:83938/104‑1 (MQ=255)
                                                                                               gtggggTGATTTGGTGGGTCGGGTGAGGGGGTTACGCCGGCTCCGGTATCAACGCCTGATGCGACGCTTGGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg                                                  <  2:100902/144‑1 (MQ=11)
                                                                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCa                                             >  2:95218/1‑149 (MQ=38)
                                                                                                      gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                    <  1:369696/76‑1 (MQ=255)
                                                                                                              aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCa                                   >  2:83009/1‑149 (MQ=38)
                                                                                                              aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGAGACGCTGGCCGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCAGCGTGATTTTGTAGGTCGGATACGGCGCTTATGCCGCATCCGACATCa                                   >  1:333631/1‑149 (MQ=11)
                                                                                                                gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                   <  2:273634/67‑1 (MQ=255)
                                                                                                                gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                   >  1:273634/1‑67 (MQ=255)
                                                                                                                      tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGa                           <  1:537941/149‑1 (MQ=37)
                                                                                                                       caGGCGTTTACGCCGCACCCGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGTCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                          <  2:638038/148‑1 (MQ=255)
                                                                                                                       aaGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                          <  2:501435/149‑1 (MQ=38)
                                                                                                                         ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                  <  1:453305/107‑1 (MQ=40)
                                                                                                                              ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGc                   <  1:83009/149‑1 (MQ=38)
                                                                                                                                          cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATAAGACGCGTTAAGCGTcgc       >  1:38152/1‑147 (MQ=11)
                                                                                                                                           gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa      >  2:616704/1‑149 (MQ=35)
                                                                                                                                            aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCttct     >  1:128681/1‑147 (MQ=25)
                                                                                                                                               tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCTTATGTCAGACTGCGCAATACCCTGAGTTCGCCTGATTTTGTCGGTCGCCTAAGGCGTTTATGCCGCTTCCGATCTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg  <  2:644821/149‑1 (MQ=11)
                                                                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc              <  1:633051/134‑1 (MQ=31)
                                                                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc              >  2:633051/1‑134 (MQ=31)
                                                                                                                                                   |                                                                                                                                                
TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAG  >  NC_000913_3_hsa_tpiA/4295895‑4296186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: