New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	4542664 =	39 (0.650)	6 (0.110)	6/264	7.2	12.3%	intergenic (+49/‑433)	fimE/fimA	tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
	?	NC_000913_3_hsa_tpiA	4542978 =	49 (0.870)					intergenic (+363/‑119)	fimE/fimA	tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTT  >  NC_000913_3_hsa_tpiA/4542831‑4543123                                                                                                                                                    |                                                                                                                                                  gACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACCACTAAGCTTAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAAt                                                                                                                                                  <  1:471102/149‑1 (MQ=255)   cGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGt                                                                                                                                                <  1:851823/149‑1 (MQ=255)    gCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGCTACCAATAAAATCTTAAGCCAACAAATAAACTGACAAAGTTTGTCCGCGATGCTTTCCTCTGATACTCCAAATGGCCCCAAATGtt                                                                                                                                               >  2:731642/1‑149 (MQ=255)    acATCTTCCTCATTCTTCTCTACAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGtt                                                                                                                                               <  2:929477/148‑1 (MQ=255)         ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATc                                                                                                                                          >  1:494987/1‑149 (MQ=255)              cATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTCCATCTTTTg                                                                                                                                     >  2:316794/1‑149 (MQ=255)              cATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCAAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTg                                                                                                                                     >  1:625449/1‑149 (MQ=255)                   ttctCTCCAAAAACCACCTAATGCAATATAAACATCTACAAATTAGGAAAACCAACGTATACGAACCCAACAAATAAACTGAAAAAGTTTGTCCGCGATCCTTCCCTCTATGAGTCAAAATGGCCCCCAATGTTCCATCTTTTGGGGGa                                                                                                                                >  2:120892/1‑149 (MQ=255)                        tcCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACt                                                                                                                           >  2:628834/1‑149 (MQ=255)                            aaaaCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGc                                                                                                                       <  1:383383/149‑1 (MQ=255)                             aaaCCACCTCATGAAATATAAAAATCTATAAATAAAGATAACAATATAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCa                                                                                                                      <  1:446250/149‑1 (MQ=255)                                ccaccTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAgtg                                                                                                                   >  1:357560/1‑149 (MQ=255)                                    cTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTg                                                                                                                                     >  2:455307/1‑127 (MQ=255)                                    cTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTg                                                                                                                                     <  1:455307/127‑1 (MQ=255)                                    cTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTgg                                                                                                               <  1:1001867/149‑1 (MQ=255)                                     tCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGCTGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGCTTCAGCTTTTGGGGGAAAACTGTGCAGAGTTGGc                                                                                                              >  1:337298/1‑149 (MQ=255)                                        tGCAAAATAAACATCTATAAATAAAGATAACAATAGAATATCAAGCCAACAAATAAACTGAAAAGGTTTGTCCGCGATGCTTTCCTCTATGAGACACAATGGCCCCAAACGTTTCATCTTTTGGGGGAAAACTGTCCAGGGTTGACAgc                                                                                                           >  2:336510/1‑148 (MQ=255)                                                    aTCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTa                                                                                               >  2:767370/1‑149 (MQ=255)                                                         ggtataaaTATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTCTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaa                                                                                          <  2:787813/146‑1 (MQ=255)                                                           aataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaaca                                                                                        >  2:379523/1‑149 (MQ=255)                                                           aataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCACAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaaca                                                                                        <  1:299402/149‑1 (MQ=255)                                                            ataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaacaa                                                                                       >  1:727565/1‑149 (MQ=255)                                                              aaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAg                                                                                     <  1:696452/149‑1 (MQ=255)                                                                aGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTAAAACCGGCCGGCACGGGTGCATCTGTTGTGCAATGACAGTGCAGTGTTGGCAGACAAACTCGTGTACAAAACAAAgtg                                                                                   <  2:1007195/149‑1 (MQ=255)                                                                  aTAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTa                                                                                 <  1:123764/149‑1 (MQ=255)                                                                       aaTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGaa                                                                            <  1:428479/149‑1 (MQ=255)                                                                        aTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCTAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAAc                                                                           <  1:932056/149‑1 (MQ=255)                                                                                  aaGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCAt                                                                 <  1:628834/149‑1 (MQ=255)                                                                                     ccAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGccc                                                                   <  2:84957/144‑1 (MQ=255)                                                                                     ccAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGccc                                                                   >  1:84957/1‑144 (MQ=255)                                                                                     ccAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTc                                                              >  2:580234/1‑149 (MQ=255)                                                                                        acaaATAAACTGAAAAAGTTTATCCGCAATACTTTCCTCTATGAGTAAAAAGGGCCCCAAATGTTTCATCTTTAGGGGGAAACCTGTGCAGTGTTGGACGTCAAACTCGTTTACAAAACTAAGAGTACACACCGACTGTCCactcggct                                                           >  2:504110/1‑142 (MQ=255)                                                                                             tAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGaa                                                                            >  1:836609/1‑127 (MQ=255)                                                                                             tAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGaa                                                                            <  2:836609/127‑1 (MQ=255)                                                                                               aaCTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGaaa                                                    >  1:11027/1‑149 (MQ=255)                                                                                                aCTGGAAAAGCTTGTCCGCGGTGCTTTCCTCGATGAGACAAAATGGCCCCAAATGATGCATCTTTTGGGGGAAAACTGTGCCGGGTTGGCAGTCAAACTTGATTACAAAACAAAGTGTCCAGAACGACTGCCCATGTCGATTTAGAAAt                                                   >  2:650620/1‑149 (MQ=255)                                                                                                aCTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAAt                                                   >  2:46007/1‑149 (MQ=255)                                                                                                       aaGTTTGTCCGCTATGCTTTCCTCTATGAGTCAAAATGGCCCTAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGTAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGttttt                                            <  2:761103/149‑1 (MQ=255)                                                                                                        aGTTTGTCCGGGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGtttttt                                           <  2:409204/149‑1 (MQ=255)                                                                                                                  cgATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCaaa                                                                                                       <  1:637101/79‑1 (MQ=255)                                                                                                                  cgATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCaaa                                                                                                       >  2:637101/1‑79 (MQ=255)                                                                                                                                aTGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTa                   >  1:386114/1‑149 (MQ=255)                                                                                                                                aTGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTa                   >  2:689474/1‑149 (MQ=255)                                                                                                                                aTGAGTAAAAAGGGTCCCAAATGTTTCATTTTTTAGGGTAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTAAAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTa                   <  2:38388/149‑1 (MQ=255)                                                                                                                                 tGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTaca                                                                                             >  2:945209/1‑74 (MQ=255)                                                                                                                                 tGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTaca                                                                                             <  1:945209/74‑1 (MQ=255)                                                                                                                                 tGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTaa                  >  2:413776/1‑149 (MQ=255)                                                                                                                                  gAGTCAAAATGGTCCCAAATTGTTCATCTTTTTGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATTCGCTGGTTAAAGGAAAGCAGGATGAAAATTaaa                 <  2:494987/149‑1 (MQ=255)                                                                                                                                     acagtttGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAAct              >  2:288296/7‑149 (MQ=255)                                                                                                                                        aaaTGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTg           <  1:46007/149‑1 (MQ=255)                                                                                                                                             gCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAAt      >  2:47913/1‑149 (MQ=255)                                                                                                                                                 cAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCgtt  <  1:413776/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTT  >  NC_000913_3_hsa_tpiA/4542831‑4543123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.