Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,100,7460AC73.1% 79.9 / 31.7 52K151Q (AAG→CAG) ycdZDUF1097 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (7/7);  new base C (23/15);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTTGTTCCGGGGACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGT  >  NC_000913_3_hsa_tpiA/1100602‑1100882
                                                                                                                                                |                                                                                                                                        
tttGTTCCGGGGACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGAc                                                                                                                                      >  2:960956/1‑149 (MQ=255)
tttGTTCCGGGGACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGAc                                                                                                                                      >  1:50093/1‑149 (MQ=255)
tttGTTCCGGGGACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGAc                                                                                                                                      >  2:835527/1‑149 (MQ=255)
   gTTCCGGGGACGTTTATAGGCGCATGCGGGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGgc                                                                                                                                   <  2:514212/149‑1 (MQ=255)
         gggACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTAGCGCTTGAGCTGATATTTGGTTACGAAACGAAAAACAGTGGCCTGTGCCTGGCGGCGCGTGGTGCACAGACGGCGCACCg                                                                                                                             >  2:302852/1‑149 (MQ=255)
          ggACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGt                                                                                                                            <  2:854183/149‑1 (MQ=255)
            gcGTTTATAGGCGCATGCGCGACGTTGGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGa                                                                                                                          <  2:36547/148‑1 (MQ=255)
             cGTTTAAAGGCGCCTGCGCGACGTTTGCCGGGCAGGGTGACTGGAAATTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTATGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAg                                                                                                                         <  1:490299/149‑1 (MQ=255)
              gTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGc                                                                                                                        >  1:588849/1‑149 (MQ=255)
               tttAGAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAATCTGGTGTTACCTTCGCTGGCGCTTGGGCCCATTTTGATTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCa                                                                                                                       <  2:643810/149‑1 (MQ=255)
                      gcgcATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGGGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATc                                                                                                                >  2:348257/1‑149 (MQ=255)
                                    tttGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCggg                                                                                                  >  2:566637/1‑147 (MQ=255)
                                       gCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAgg                                                                                               <  1:650223/149‑1 (MQ=255)
                                                ggTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCAc                                                                                      <  2:838864/149‑1 (MQ=255)
                                                      tGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTc                                                                                >  1:402047/1‑149 (MQ=255)
                                                      tGGAAACTGGTGGTACCTTCGCTGGCGGTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGGGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTc                                                                                <  2:520769/149‑1 (MQ=255)
                                                        gAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGc                                                                              >  2:330937/1‑149 (MQ=255)
                                                        gAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGc                                                                              >  1:845089/1‑149 (MQ=255)
                                                                tgtTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAg                                                                      <  2:402047/149‑1 (MQ=255)
                                                                 gtTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGt                                                                     >  1:422771/1‑149 (MQ=255)
                                                                  ttACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTa                                                                    >  1:847566/1‑149 (MQ=255)
                                                                    aCCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTaaa                                                                  >  1:758452/1‑149 (MQ=255)
                                                                     ccTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTaaaa                                                                 >  1:708180/1‑149 (MQ=255)
                                                                         cGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGcc                                                             <  1:835527/149‑1 (MQ=255)
                                                                          gCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCg                                                            <  1:330937/149‑1 (MQ=255)
                                                                                gcTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgatgat                                                      >  1:352642/1‑149 (MQ=255)
                                                                                      gCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTcc                                                >  1:1013852/1‑149 (MQ=255)
                                                                                        tGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCgg                                              >  1:46401/1‑149 (MQ=255)
                                                                                        ggATATTGGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTCCGCTTAAACAGTAAAATGCCGCATGATAATTCCgg                                              <  2:549936/148‑1 (MQ=255)
                                                                                         gATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGc                                             >  1:855660/1‑149 (MQ=255)
                                                                                                 gTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTAt                                     >  1:535678/1‑149 (MQ=255)
                                                                                                  ttACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATc                                    <  2:50093/149‑1 (MQ=255)
                                                                                                   tACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCt                                   <  2:588849/149‑1 (MQ=255)
                                                                                                      gCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTc                                >  1:1048254/1‑149 (MQ=255)
                                                                                                      gCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTc                                >  1:675226/1‑149 (MQ=255)
                                                                                                       cAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCa                               >  1:197604/1‑149 (MQ=255)
                                                                                                       cAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCa                               <  1:76357/149‑1 (MQ=255)
                                                                                                         aTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGAGGATAATTCCGGCTTCTTTATCTGTCAgg                             >  2:668616/1‑149 (MQ=255)
                                                                                                          tGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCTTCTTTGTTCTCTGTCATGa                            <  2:758452/149‑1 (MQ=255)
                                                                                                           gAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCCAAAATAAAGCGTGAGGGGCACTCACGCTTTAGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTCTTTATCTGtatggtt                           >  2:811149/1‑143 (MQ=255)
                                                                                                           gAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGACTTTTTTATCTGTCAGGAt                           >  1:457245/1‑149 (MQ=255)
                                                                                                           gAAAAACAGTGGCCTGTGGCTGGCGACGCGAGTTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGAt                           >  1:1037930/1‑149 (MQ=255)
                                                                                                                aCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCgg                      <  2:352642/149‑1 (MQ=255)
                                                                                                                      gCCTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAAc                >  1:1034162/1‑149 (MQ=255)
                                                                                                                       ccTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTa                                                                    <  2:166954/96‑1 (MQ=255)
                                                                                                                       ccTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTa                                                                    >  1:166954/1‑96 (MQ=255)
                                                                                                                        cTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTaaa                                                                         >  1:331113/1‑90 (MQ=255)
                                                                                                                        cTGTGGCTGGCGGCGCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTaaa                                                                         <  2:331113/90‑1 (MQ=255)
                                                                                                                          ggggctggctGGGCGTAGGGGACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGAc            <  1:747090/147‑1 (MQ=255)
                                                                                                                                 gcggcgCGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGc     >  1:829075/1‑149 (MQ=255)
                                                                                                                                 gcggcgCGTAGTGCAAAGACGGCGGACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGc     <  1:925456/149‑1 (MQ=255)
                                                                                                                                    gcgcGTAGTGCACAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGt  <  2:422771/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
TTTGTTCCGGGGACGTTTATAGGCGCATGCGCGACGTTTGCCGGGCAGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGT  >  NC_000913_3_hsa_tpiA/1100602‑1100882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑