Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,815,8090G.25.4% 134.2 / 48.8 56intergenic (‑41/+25)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (21/20);  new base . (4/10);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAA  >  NC_000913_3_hsa_tpiA/3815662‑3815957
                                                                                                                                                   |                                                                                                                                                    
ttGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAgg                                                                                                                                                     <  1:453316/149‑1 (MQ=255)
ttGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAgg                                                                                                                                                     <  1:785302/149‑1 (MQ=255)
  gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTGGCTCCTCATCTTACTTTTCTACAG‑CAAAAAAAAgggcg                                                                                                                                                  >  1:257433/1‑146 (MQ=255)
      aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGTCACAAAAAA‑GCGACtca                                                                                                                                              >  2:143225/1‑149 (MQ=255)
        aTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtgat                                                                                                                                             >  1:287286/1‑149 (MQ=255)
                 tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGATTCGCCt                                                                                                                                    <  2:287286/149‑1 (MQ=255)
                       cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAGATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTTCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTcaacc                                                                                                                              >  1:310663/1‑147 (MQ=255)
                        ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCGTCAGTCCCCTTAAATATc                                                                                                                            >  1:138020/1‑149 (MQ=255)
                          ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCAGCTTACCTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCa                                                                                                                           <  1:732891/149‑1 (MQ=255)
                              ttCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTGTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGttt                                                                                                                       <  2:150333/149‑1 (MQ=255)
                                  gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTCCCa                                                                                                                   >  2:449136/1‑149 (MQ=255)
                                   cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAg                                                                                                                  >  2:21212/1‑149 (MQ=255)
                                    gTAAACTCGCCAAACTTTAACACCTGCTTACTAGGCGCAAATTCAGTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAAACAAAAAAAAACGGAATCATCAGTCGCCTTAAAAATCAGTTTGCAAgc                                                                                                                 >  2:272191/1‑149 (MQ=255)
                                      aaaaTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                              <  1:875236/149‑1 (MQ=255)
                                       aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAACTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCCGTTTGCCGGcgcc                                                                                                              >  1:332830/1‑149 (MQ=255)
                                              cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                       >  1:567540/1‑149 (MQ=255)
                                               aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                                      >  2:682926/1‑149 (MQ=255)
                                               aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                                      >  1:809416/1‑149 (MQ=255)
                                                        cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                             <  1:841569/149‑1 (MQ=255)
                                                        cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCACTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                             >  1:989222/1‑149 (MQ=255)
                                                          ccggcttgctAAGCGCAAATTCAATCACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACaa                                                                                           <  1:738447/146‑1 (MQ=255)
                                                          ccTGCTTGCTAAGCGCAAATTCAATAAACTGGTGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTCCTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGTCTGCGTCGCTACaa                                                                                           >  1:64624/1‑149 (MQ=255)
                                                              cttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                                                                       <  1:468205/149‑1 (MQ=255)
                                                               ttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                      <  1:682926/149‑1 (MQ=255)
                                                               ttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgatt                                                                                     <  1:888084/149‑1 (MQ=255)
                                                                  ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTAGCCTTAAAAATCAGt                                                                                                                         >  2:695804/1‑111 (MQ=255)
                                                                  ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCGTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGt                                                                                                                         <  1:695804/111‑1 (MQ=255)
                                                                   taggcgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                                  <  2:820235/146‑1 (MQ=255)
                                                                   tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgg                                                                                  >  1:479235/1‑148 (MQ=255)
                                                                   tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                                  <  1:639308/149‑1 (MQ=255)
                                                                      gcgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATCgattcgattc                                                                               >  2:336612/1‑149 (MQ=255)
                                                                       cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGaa                                                                                                                                                 <  1:461366/82‑2 (MQ=255)
                                                                       cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGaa                                                                                                                                                 >  2:461366/1‑81 (MQ=255)
                                                                            aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                        <  1:391075/149‑1 (MQ=255)
                                                                             ttCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                        >  2:230397/1‑149 (MQ=255)
                                                                                  tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                                  <  1:396135/149‑1 (MQ=255)
                                                                                   aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                                  <  2:567540/149‑1 (MQ=255)
                                                                                     aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCGTGTAACTTTTCTACAGACAAAAAAAAGGAAAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCCGGCCAGGGc                                                                >  2:739691/1‑149 (MQ=255)
                                                                                     aCTGGCGCTGATATGGGTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCACCGCCGCCTTCTGCGTCGCTACAATGGATTCGATCCCCCCCGGGCCCGAccc                                                               >  2:654677/1‑149 (MQ=255)
                                                                                          cgcTGATGAGGTGTTATGTCTTCGCACTGCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGGCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaacaa                                                          <  2:163754/149‑1 (MQ=255)
                                                                                             tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                        <  1:422461/149‑1 (MQ=255)
                                                                                             tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAt                                                       <  1:307643/149‑1 (MQ=255)
                                                                                              gATATTGTTTCATGCTTTCGACTGTCATCTTGCTTTACAATGGACAAAAATAGGGCTGATCATAAGTCGACTAAAAAAACAGTTGGCGAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGGCAACAAGAt                                                       <  2:658754/149‑1 (MQ=255)
                                                                                                 tgggTTTAATGCCTTCGTTCCTCACTTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                   <  2:138020/147‑1 (MQ=255)
                                                                                                   ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTa                                                  >  1:189194/1‑149 (MQ=255)
                                                                                                     tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                               >  1:1042715/1‑149 (MQ=255)
                                                                                                         aTGCGTTTGCTCTTCATCTTACGTTTCTAAAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTc                                           <  2:548486/149‑1 (MQ=255)
                                                                                                         aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                            >  2:331472/1‑149 (MQ=255)
                                                                                                          tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTc                                           >  2:365289/1‑149 (MQ=255)
                                                                                                           gattgcGCTTCTCATCTTAGCTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                          <  2:989222/144‑1 (MQ=255)
                                                                                                              ttCGCTCCTCATCTTACTTTTCTACATACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                       <  1:238730/149‑1 (MQ=255)
                                                                                                                     ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                                <  1:331472/149‑1 (MQ=255)
                                                                                                                         tCTTATTTTTCCACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTATAATGGATTCTATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGTTc                            <  2:155996/149‑1 (MQ=255)
                                                                                                                            tACTTTTCTACAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTCGCTCTTCATGGGTGAACGGCTCGcc                         >  2:293961/1‑149 (MQ=255)
                                                                                                                                  tCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                  <  1:241653/149‑1 (MQ=255)
                                                                                                                                     aCAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc               <  2:44691/149‑1 (MQ=255)
                                                                                                                                      cAGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc               <  2:189194/149‑1 (MQ=255)
                                                                                                                                       aGACAAAAAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcc              >  2:889264/1‑149 (MQ=255)
                                                                                                                                           caaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg          <  1:1010040/148‑1 (MQ=255)
                                                                                                                                            aaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTTCCGTCCCCTGc         >  1:531864/1‑149 (MQ=255)
                                                                                                                                             aaaaaaGGCGAATCATCAGTCGCGTTAAGAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAGCGGGTCGCCTTCTGCCGTCCCCTGCa        <  2:310663/149‑1 (MQ=255)
                                                                                                                                                   ggCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCaa  <  1:230397/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAA  >  NC_000913_3_hsa_tpiA/3815662‑3815957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑