Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,111,662 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,111,6620GA97.0% 206.8 / NA 68L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/27);  minor base C (1/1);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGA  >  NC_000913_3_hsa_tpiA/4111519‑4111805
                                                                                                                                               |                                                                                                                                               
aGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCt                                                                                                                                            >  1:320928/1‑149 (MQ=255)
  gAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCttt                                                                                                                                          >  1:531426/1‑149 (MQ=255)
      gtGGCCGCCCCGCAGGGCAGCAGGGCTGTGAAACAGTATAGGGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGc                                                                                                                                      <  2:301883/148‑1 (MQ=255)
       tGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGcc                                                                                                                                     <  2:316126/149‑1 (MQ=255)
          ccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTGAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGtt                                                                                                                                  <  2:1044987/149‑1 (MQ=255)
            gccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGt                                                                                                                                >  2:982762/1‑149 (MQ=255)
                             gTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                               >  2:903124/1‑149 (MQ=255)
                             gTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGGCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                               <  2:846734/149‑1 (MQ=255)
                             gTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTATTCTCTTGTTTTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                               >  2:270739/1‑149 (MQ=255)
                              tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                              <  1:962594/149‑1 (MQ=255)
                              tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                              <  1:757364/149‑1 (MQ=255)
                               cTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAg                                                                                                             <  1:982762/149‑1 (MQ=255)
                                tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCTAGCGATTCAGATAGCGCCAGCTTACTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                            >  2:647475/1‑149 (MQ=255)
                                tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                          >  2:972618/1‑135 (MQ=255)
                                tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                          <  1:972618/135‑1 (MQ=255)
                                  tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTTTCATTTATTCAAACCTACAAGCGAGTAAGATAGCGACCGCTCATTTGGTTCAACTGCGCGGGTAAACTCCTCTCCGCCGTGTTCCGCCAAAACATAAcccgtgac                                                                                                          >  2:658724/1‑142 (MQ=255)
                                  ggAAACAGTATAGGGATTCATCGGCACAAATGCTTTGCTTTTTGTCATTTATTCAACCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCGGTTCGCCGTTGTCCGCGACAACATAACGCAGTGc                                                                                                          <  2:752801/148‑1 (MQ=255)
                                         gTATAGAGATTCATCGTCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt                                                                                                   <  1:962277/149‑1 (MQ=255)
                                          tataGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTg                                                                                                  >  2:208010/1‑149 (MQ=255)
                                                 aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgt                                                                                           >  2:778613/1‑149 (MQ=255)
                                                     aTCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                          <  2:493302/114‑1 (MQ=255)
                                                     aTCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                          >  1:493302/1‑114 (MQ=255)
                                                      tCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGtaat                                                                                      >  2:369139/1‑149 (MQ=255)
                                                         gCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCAGTTCAACAGCAAAGGTCAACCCCTTTTCGCCGGTGTCAGCCACCCCCTAACGCACTGCACCCCCTGCCTCGGTGtactcaa                                                                                   >  2:160748/1‑144 (MQ=255)
                                                           acaAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAAGCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgt                                                                                 >  1:920901/1‑149 (MQ=255)
                                                            caAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGCCAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtt                                                                                <  1:778613/149‑1 (MQ=255)
                                                             aaaGGCTTTGCTTTTTTTTTTTTTTTCAAACCTTCAAGCGATTCAGATAGCGCCACCTTAATCGGTTCAACAGCGGAGGTCACCCCCTTTTCGCCCTTGTCCGCGACAACATAACGCTTTTCACCTTCTGTCTCCTTGTAATAACgttt                                                                               >  2:13106/1‑149 (MQ=255)
                                                             aaaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAGgttt                                                                               >  1:475445/1‑149 (MQ=255)
                                                                ggCTTTGCTATTTGTCATTAAGCCAAGCCTTGACCTGCCGCAAACAGCGCCAGCTTAATCGATTCAACACCGCAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAAAGCATTGCACCTCATGTCTCCGCGTACTAACgattgtt                                                                            >  2:446142/1‑149 (MQ=255)
                                                                   tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGttttt                                                                         >  1:937042/1‑149 (MQ=255)
                                                                    ttgctttATGTCAGTTATTCAAACCTTCAAGGGATTCCGATAGTGCCAGCTTAATCGGTTCCACAGCGAAGGTGAACCCCTCTTCGCCGTTGTTCGCGAAAACATACCGAAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGGTTTTc                                                                        <  2:497539/149‑1 (MQ=255)
                                                                       ctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcccc                                                                     >  1:187172/1‑149 (MQ=255)
                                                                                aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACCGCGAGGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACTTTCTGTCTCGGTGTAATAGCGTTTGTTTTTCCccgcatgtaac                                                            >  2:304578/1‑142 (MQ=255)
                                                                                aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                            >  1:969945/1‑149 (MQ=255)
                                                                                aTTTATTAAAACCGTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCCCCTTCTTTCTCGGTGTAATAACGTTTGTTTTTCCCCGGCGTtcgt                                                            >  2:497483/1‑149 (MQ=255)
                                                                                 tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagta                                                           >  1:918340/1‑149 (MQ=255)
                                                                                 tatATTCAAACCTTCAAGAAATGCAGATAGCGCGAGTTTAATCGTTTCAACAGCGAAGGTCAACCCCTATTCGCCGTTGTCCGCGACAACATAACGCAGTGCCCCTTCTGGCTCGGTGGAATAACGTTGGTTTTTCCTCCCCGtaggta                                                           >  2:9902/3‑144 (MQ=255)
                                                                                        aaaCCTTCAAGCGATTCAGATAGCGCCAGGTTAATCGGTTCAACCGCGAAGGTCCACCCCTATTCGCCGTAGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTATTCCCCGCCGTTAGTAGCTACTg                                                    >  1:884780/1‑149 (MQ=255)
                                                                                          aCCTTCACGCGATTCAGATAGCGCCAGCTTACTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCa                                                  <  1:670734/149‑1 (MQ=255)
                                                                                                aaGCGATTCAGATAGCTCCAGCGTACGCGTTGCAATAGGTATGTTCCACTTCTTTACGCCGTTGTCGGAGACAATATAACACAGTTCACCCTCGGTATCGGTGTAAGAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                            <  2:475445/149‑1 (MQ=255)
                                                                                                  gCGATTCAGATAGCGCCATCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                          <  1:400734/149‑1 (MQ=255)
                                                                                                  gCGAATCAGTAAGCGCCAGCTTAATCGGATCAACGGCGATGGTCATCCCCTTTTCGCCCCTGTCCGCGACAACAAAAGGCCGTGCACCTTCGGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCCACTGCAGTTTTTTc                                          <  2:125158/149‑1 (MQ=255)
                                                                                                    gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTg                                        >  2:667323/1‑149 (MQ=255)
                                                                                                    gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTg                                        <  1:903124/149‑1 (MQ=255)
                                                                                                              gcgcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTATGGCTTTGTGtt                              >  2:536557/1‑149 (MQ=255)
                                                                                                               cgcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGttt                             <  1:644254/149‑1 (MQ=255)
                                                                                                                gcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                            >  2:81514/1‑149 (MQ=255)
                                                                                                                gcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGCCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                            <  1:796758/149‑1 (MQ=255)
                                                                                                                 ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCgct                                                                                           >  2:364477/1‑83 (MQ=255)
                                                                                                                 ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCgct                                                                                           <  1:364477/85‑3 (MQ=255)
                                                                                                                 ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                           <  1:283560/149‑1 (MQ=255)
                                                                                                                  aaGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTgg                          <  1:24282/148‑1 (MQ=255)
                                                                                                                   gacTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTCCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGTGACTGCAGGTTTTTCTGGCTTTGTGTTTTGGt                         <  1:830709/147‑1 (MQ=255)
                                                                                                                   accgtAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACCACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                         <  1:87404/145‑1 (MQ=255)
                                                                                                                        aaTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa                    >  2:541623/1‑149 (MQ=255)
                                                                                                                          tCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtcgt                                                            >  1:460573/1‑107 (MQ=255)
                                                                                                                          tCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                            <  2:460573/107‑1 (MQ=255)
                                                                                                                           cgttgcAACAGCGAAGGTCACCCCGTTTTCGCCGTTGTCTGCTACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATg                 <  2:187172/144‑1 (MQ=255)
                                                                                                                             gTTCAACAGCGAAGGTCAACCCCTTTTTGCCGTTGTCCGCGACAATATAACGCAGTGCACCTTCTGTCTCAGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg               <  2:884780/149‑1 (MQ=255)
                                                                                                                             gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTTTGGCTTTGTGTTTTGGTCATTAATggg               >  2:772122/1‑149 (MQ=255)
                                                                                                                                 aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTCATAACGTTAGTTTTTGCCCGCCGTGACTAGCGGCTGCAGTTCTTTCTGGCTTTGAGTGTTGTGCATTAATGGGGTGa           >  2:463561/1‑149 (MQ=255)
                                                                                                                                 aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa           >  2:503854/1‑149 (MQ=255)
                                                                                                                                  aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTTTGGATATGTGTTTTGGTCATTAATGGGGTGAg          >  2:435820/1‑149 (MQ=255)
                                                                                                                                  aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTCATGTCCTTTTGCATTTTTGTCTGTCTTTGTGTTTTGGTCATTACTGCGGTGAg          >  2:638080/1‑149 (MQ=255)
                                                                                                                                       gAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc     <  1:667323/149‑1 (MQ=255)
                                                                                                                                        aaGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCg    >  2:213824/1‑149 (MQ=255)
                                                                                                                                        aaGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGCCTTTGTGTCTGGGTCATTAATGGGGTGAGTGTGCg    >  2:569491/1‑149 (MQ=255)
                                                                                                                                          ggTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGGGGa  >  1:155847/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGA  >  NC_000913_3_hsa_tpiA/4111519‑4111805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑