Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,183,186 G→C D654H (GAC→CAC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,183,1860GC81.6% 193.5 / 31.3 78D654H (GAC→CAC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (10/4);  new base C (36/26);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAC  >  NC_000913_3_hsa_tpiA/4183039‑4183331
                                                                                                                                                   |                                                                                                                                                 
tACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGa                                                                                                                                                  >  1:153970/1‑149 (MQ=255)
 aCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACTAGGTTGACTACATGGAc                                                                                                                                                 <  1:539629/149‑1 (MQ=255)
  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGCAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGCCg                                                                                                                                                >  1:72144/1‑149 (MQ=255)
      tCTGCTATCGAAGAAGGCCACTGCTTTAGCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCTAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATc                                                                                                                                            <  2:216870/149‑1 (MQ=255)
      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAta                                                                                                                                            >  1:572713/1‑148 (MQ=255)
      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATc                                                                                                                                            >  1:304875/1‑149 (MQ=255)
        tGCTATCGAAGAAGGCAACTCCGTTATCGCCCAGGCGAACTCCATCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTTTCCa                                                                                                                                          >  2:906453/1‑149 (MQ=255)
         gCTATCGAAGAAGGCAACTACGTTACCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCAc                                                                                                                                         <  1:628764/149‑1 (MQ=255)
          cTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCAcc                                                                                                                                        >  2:649225/1‑149 (MQ=255)
          cTATCGAAGAAGGCAAATACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCAcc                                                                                                                                        >  1:914104/1‑149 (MQ=255)
            aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTCTCCACCca                                                                                                                                      >  2:398490/1‑149 (MQ=255)
               gaagaaGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCcagca                                                                                                                                   >  2:156897/1‑149 (MQ=255)
                aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCcagcag                                                                                                                                  <  1:633534/149‑1 (MQ=255)
                   aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGTCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAggtg                                                                                                                               <  2:309493/149‑1 (MQ=255)
                   aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAggtg                                                                                                                               <  1:278932/149‑1 (MQ=255)
                       cAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAACAggtggtct                                                                                                                           >  2:412979/1‑147 (MQ=255)
                         aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATcc                                                                                                                         <  2:428772/149‑1 (MQ=255)
                             cGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCg                                                                                                                     >  2:681871/1‑149 (MQ=255)
                               ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCCTCGGt                                                                                                                   >  1:298209/1‑149 (MQ=255)
                               ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGt                                                                                                                   <  2:504494/149‑1 (MQ=255)
                                  tCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACGTGGTAACTTTCCGTGGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCACGTGGTATCCGTCGGTACg                                                                                                                >  1:988075/1‑149 (MQ=255)
                                   cGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGt                                                                                                               <  2:139126/149‑1 (MQ=255)
                                     cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGGGGTATCCGACGGTGCGTcc                                                                                                             >  1:1047250/1‑149 (MQ=255)
                                      ccAGGCTAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                            <  1:62024/149‑1 (MQ=255)
                                      acAGGCGAACTCTAACTTAGATTGAGAATGGCCTTTCGTAGAAGAACTGTTAACTTGCCGTAGCAAAGGCCAATCCAGCTTTTTCAGCCGCGACTAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                            <  2:59655/148‑1 (MQ=255)
                                        aGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTg                                                                                                          >  1:694/1‑149 (MQ=255)
                                               cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGt                                                                                                   >  2:252046/1‑149 (MQ=255)
                                                  cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTcc                                                                                                >  2:660550/1‑149 (MQ=255)
                                                  cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGGTcc                                                                                                >  1:1044729/1‑149 (MQ=255)
                                                    aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTg                                                                                              <  1:64943/149‑1 (MQ=255)
                                                        ggATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTTGAac                                                                                          >  2:661429/1‑149 (MQ=255)
                                                           tGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGAACCCGTTCCTGGAACACg                                                                                       >  2:468711/1‑149 (MQ=255)
                                                             aagaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                                                                     <  1:649225/149‑1 (MQ=255)
                                                             aagaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                                                                     <  1:156897/149‑1 (MQ=255)
                                                                     cACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGt                                                                                                               >  2:802884/1‑115 (MQ=255)
                                                                     cACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGt                                                                                                               <  1:802884/115‑1 (MQ=255)
                                                                     cACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                             >  1:344425/1‑149 (MQ=255)
                                                                         tCGTAGAAGCCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCGGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGt                                                                         <  2:93712/149‑1 (MQ=255)
                                                                          cGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTg                                                                        >  2:196722/1‑149 (MQ=255)
                                                                           gTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGCATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTCCCTGGAACACGATGACACCAAccacac                                                                       >  1:521100/1‑145 (MQ=255)
                                                                            tAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCa                                                                      >  2:646415/1‑149 (MQ=255)
                                                                              gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCCGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGAGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                    >  1:979257/1‑149 (MQ=255)
                                                                                 gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGc                                                                                >  1:80612/1‑134 (MQ=255)
                                                                                 gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGc                                                                                <  2:80612/134‑1 (MQ=255)
                                                                                     tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTa                                                                                                                            <  1:432014/86‑1 (MQ=255)
                                                                                     tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTa                                                                                                                            >  2:432014/1‑86 (MQ=255)
                                                                                      ggTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGGCCCTGATCCCGTTCCTGTAACACGATGACGCCAACCGTGCATTGATGGGTg                                                            >  1:349772/1‑149 (MQ=255)
                                                                                           cTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAAc                                                       <  1:681871/149‑1 (MQ=255)
                                                                                            ttGCCGTAGCAAAGGCGAATCCAGCTTGATCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                      >  2:331109/1‑149 (MQ=255)
                                                                                               ccGTAGAAAAGGCGAAACCAGATTGTTAAGAGGCAATCAGGCCGAAGACAGGAACGAATAAACCCACCAGGGAGGATCCGTAGGGGCTTGCCGTATCCCGTTCCTGGAGCACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGc                                                   <  2:514497/149‑1 (MQ=255)
                                                                                                     cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGCTCCCGTCACTGCAATACAATGACGCGCACCGTGCCTTGATGGCTGCGAACATGCGACGTc                                             >  2:638100/1‑149 (MQ=255)
                                                                                                     cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGGCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAGTGGTAGGTGCGGACATGCAACGtg                                             >  1:737828/1‑148 (MQ=255)
                                                                                                      aaaGGCCAACCCAGCTTGTTCGCCCGCGCCCAGGTTGACTACATGCACGTATCCCCCCAGCAGGGGGTATCCGTCGGGGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                            <  2:424305/149‑1 (MQ=255)
                                                                                                       aaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                           >  1:791453/1‑149 (MQ=255)
                                                                                                        aGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                          >  1:191194/1‑149 (MQ=255)
                                                                                                          gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGCCGCAAACAGTGAATGGATGGGTGCGAACATGCAACGTCAGGcc                                        >  1:290412/1‑149 (MQ=255)
                                                                                                          gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGcc                                        >  2:45035/1‑149 (MQ=255)
                                                                                                           tcctcccAGCTTGTTCAGCCCCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCg                                       <  1:236979/144‑1 (MQ=255)
                                                                                                               tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTCCATTGATGCGTGCGAACATGCAACGTCCGGCCGtaga                                   >  2:236979/1‑146 (MQ=255)
                                                                                                                 cggCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                 >  1:611040/3‑149 (MQ=255)
                                                                                                                  aGCTTGTTTAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGGTCCCGATCCTGGAACACGATGACGCCAACCATGCATTGATGGGTGGGTACGTGCAATGTCAGGCGGTTCCGAc                                >  1:276009/1‑149 (MQ=255)
                                                                                                                  aGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCgcc                                >  1:445945/1‑147 (MQ=255)
                                                                                                                  aGCTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCAATGCGTCCCTGATCCCGTTCCTGGAACACGATAGCGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAc                                >  2:990638/1‑149 (MQ=255)
                                                                                                                    cTTGTTCAGCCGCGACCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActc                              >  1:31653/1‑149 (MQ=255)
                                                                                                                                  agcAGGTTGACTACATGCACGTATCCGGTCGGCAGGTGGTATCCGTTGGTGCGTCCGTGATTCCGTGCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGGCTCTGCGCGCTGATAAg                <  2:954150/147‑1 (MQ=255)
                                                                                                                                  aCCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                           >  1:261906/1‑122 (MQ=255)
                                                                                                                                  aCCAGGTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                           <  2:261906/122‑1 (MQ=255)
                                                                                                                                   ccAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTCCCGACTCTGCGCGCTGATAAGc               >  2:670910/1‑149 (MQ=255)
                                                                                                                                      ggTTCACTACCTGCACGTACCCACCCAGCCGGGGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGc            <  1:468711/149‑1 (MQ=255)
                                                                                                                                       gTTGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt           >  1:451301/1‑149 (MQ=255)
                                                                                                                                       gTTGACTACATGAACGTAGCGACCCAGTAGGTGGTATCCGGCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGGGCATTGATGAGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAGAAGCCGCt           <  1:638100/149‑1 (MQ=255)
                                                                                                                                        ttGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGTATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg          <  2:914104/149‑1 (MQ=255)
                                                                                                                                        ttGACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg          <  1:572281/149‑1 (MQ=255)
                                                                                                                                          gACTACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt        <  1:196722/149‑1 (MQ=255)
                                                                                                                                           aCTACATGCACGCATCCACCCAGCACGGGGTATCCGTCGGTGCGTCCCTGATCCCATTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCAAACATGCAACGTCAGGCCGTTCCGGCTCTGCGCGCTGATAAGCCGCtggtt       <  2:578060/149‑1 (MQ=255)
                                                                                                                                             tACATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCGGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg     >  2:326873/1‑149 (MQ=255)
                                                                                                                                               cATGCACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGTAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa   <  2:349772/149‑1 (MQ=255)
                                                                                                                                                aTGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc  <  1:646415/149‑1 (MQ=255)
                                                                                                                                                   gACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                           <  1:32194/105‑1 (MQ=255)
                                                                                                                                                   gACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                           >  2:32194/1‑105 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAC  >  NC_000913_3_hsa_tpiA/4183039‑4183331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑