Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503–1979270	1979278	9–776	26 [0]	[0] 26	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	23 (0.630)	22/264	0.8	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATA  >  NC_000913_3_hsa_tpiA/1979140‑1979422                                                                                                                                            |                                                                                                                                                tctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCAt                                                                                                                                        >  1:678151‑M2/140‑149 (MQ=255)         cttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgt                                                                                                                                <  1:427149‑M2/18‑1 (MQ=255)                     ttttcctaaatcgacgcaactgtactcgtacctgattaaacagacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAtt                                                                                                                    >  1:163587‑M2/120‑149 (MQ=255)                           taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgatttccaataatgcgtgatgcagatcacacaaaccactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAatt                                                                                                              >  1:656426‑M2/114‑148 (MQ=255)                                  acgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcactactgcgtgatgcagatcacacaaaacaatcaattacttaaccaaaatgTATAAGTCATACTGTTTTTGTGGTTGTATTTCAATCTTTTtaa                                                                                                       >  2:473409‑M2/107‑149 (MQ=255)                                      aactgtactcgtcactacacgcacatacaaaggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaaaactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCATTCTGTTAAAAAGt                                                                                                   >  1:570796‑M2/103‑149 (MQ=255)                                         tgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATCAGTCATACTTTTGTCTTGGGTGTATTTCAATCTGTTAACAAGgttt                                                                                                >  1:121617‑M2/100‑149 (MQ=255)                                                  cactaccagcacataaaacggaggggggtggtgattttcaataatgcgggatgcagatcacaaaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                                                                       <  2:312791‑M2/59‑1 (MQ=255)                                                        acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                 >  1:254812‑M2/85‑149 (MQ=255)                                                              atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaa                                                                           >  1:386975‑M2/79‑149 (MQ=255)                                                                  aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGa                                                                       >  1:487039‑M2/75‑149 (MQ=255)                                                                    cggaggggggctgctattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGAATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                     <  2:570796‑M2/77‑1 (MQ=255)                                                                         gggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGa                                                                >  1:242333‑M2/68‑149 (MQ=255)                                                                              ctgctattttcaataatgcgtgatgcagatcacacaaaacactcaattacctaacataaatgTACAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                           <  2:297567‑M2/87‑1 (MQ=255)                                                                              ctgcgattttcaataacgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTGTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                           <  1:612883‑M2/87‑1 (MQ=255)                                                                               tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTATTTTGGGTGTATTTCAATCTGTTAAAAGGTTTTTCGCTACGCTAGAAAGCGAAAATGAAACAggaaaaaac                                                          >  1:309208‑M2/62‑147 (MQ=255)                                                                                      ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATgg                                                   <  2:20442‑M2/95‑1 (MQ=255)                                                                                          ataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg                                               >  1:624238‑M2/51‑149 (MQ=255)                                                                                                cgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTTTCTTTTTTTTCAAGAAAAAATGAAACAGGAATAATCGAAATGTGATGTtgcgt                                         >  2:466900‑M2/45‑148 (MQ=255)                                                                                                 gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAACAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                        >  2:77170‑M2/44‑149 (MQ=255)                                                                                                         gatcacacaaaacactcaattacctaacataaatgTAGAAGTACTCATGCATTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaa                                <  1:662754‑M2/114‑1 (MQ=255)                                                                                                                  aaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCAc                       <  1:390498‑M2/123‑1 (MQ=255)                                                                                                                    acaatcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCg                     <  1:229348‑M2/125‑1 (MQ=255)                                                                                                                                       aaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCtata  <  2:242333‑M2/144‑1 (MQ=255)                                                                                                                                         atgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg                                               >  2:388835‑M2/4‑102 (MQ=255)                                                                                                                                         atgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg                                               <  1:388835‑M2/99‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                CGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATA  >  NC_000913_3_hsa_tpiA/1979140‑1979422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑