New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 761670	43 (1.220)	4 (0.120)	4/270	4.3	8.7%	coding (149/1218 nt)	sucB	dihydrolipoyltranssuccinase
	?	NC_000913_3_hsa_tpiA	= 761713	43 (1.260)					coding (192/1218 nt)	sucB	dihydrolipoyltranssuccinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAGCCGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGAAGAGCAAAACAACGATGC  >  NC_000913_3_hsa_tpiA/761565‑761859                                                                                                                                                     |                                                                                                                                                    gaGCCGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGaa                                                                                                                                                     <  2:251559/148‑1 (MQ=255)  aGCCGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAg                                                                                                                                                    <  1:573705/149‑1 (MQ=255)  aGCCGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTAATGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAg                                                                                                                                                    <  1:279614/149‑1 (MQ=255)   gCCGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAgg                                                                                                                                                   >  1:9296/1‑149 (MQ=255)    ccGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGAGATGGCTGGAGTCGAAACTGGGAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAGGGt                                                                                                                                                  >  1:304413/1‑149 (MQ=255)           cACCGTCGCAACCTGGCATAAAAAACACGGAGACGCAGTCGTACGTGATGAAGTGCTGTGAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACgg                                                                                                                                           >  2:281718/1‑149 (MQ=255)               gTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAAc                                                                                                                                      <  2:485770/149‑1 (MQ=255)                          gCAGAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGa                                                                                                                           <  2:519045/149‑1 (MQ=255)                                aaaaCCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTg                                                                                                                     >  1:152488/1‑149 (MQ=255)                                 acaCCCGGCGACGCAGTCGTACGTGATGCAGTGCTGGTAGAAATCGAAACTGACAAACGGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTgg                                                                                                                    <  1:502103/147‑1 (MQ=255)                                              cAGGCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGaa                                                                                                       <  2:449561/149‑1 (MQ=255)                                                 tCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGc                                                                                                    >  1:530698/1‑149 (MQ=255)                                                    tACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAAc                                                                                                 <  2:97664/149‑1 (MQ=255)                                                     aCGTGATGAAGTGCCGGGAGAAATCGAAACTGACAAAGGGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACa                                                                                                <  2:76757/149‑1 (MQ=255)                                                           tgaAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCg                                                                                          <  1:379216/149‑1 (MQ=255)                                                                 gCTGGGAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTTTGTGAAGGCAACAGCGCCGGTAAAg                                                                                    <  1:281718/149‑1 (MQ=255)                                                                   tGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGaa                                                                                  <  2:479881/149‑1 (MQ=255)                                                                          aaTCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAacg                                                                           >  2:391955/1‑149 (MQ=255)                                                                            tCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGcc                                                                         >  1:418190/1‑149 (MQ=255)                                                                              gAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCaa                                                                       >  1:138112/1‑149 (MQ=255)                                                                                aaCTGACAAAGTGGTACTGGAAGTACCGACATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCAGGTAAAGCAACCAACGCCAAAt                                                                     >  2:186582/1‑149 (MQ=255)                                                                                   tGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTg                                                                  <  1:617676/149‑1 (MQ=255)                                                                                   tGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTg                                                                  >  1:608208/1‑149 (MQ=255)                                                                                     aCAAAGGTGTACGGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGaa                                                                <  2:242466/149‑1 (MQ=255)                                                                                       aaaGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAaga                                                              >  1:242893/1‑149 (MQ=255)                                                                                          gTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGaa                                                           <  2:537556/149‑1 (MQ=255)                                                                                                 tGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAAcc                                                                               >  2:414106/1‑122 (MQ=255)                                                                                                 tGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAAcc                                                                               <  1:414106/122‑1 (MQ=255)                                                                                                 tGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTcc                                                    >  2:80048/1‑149 (MQ=255)                                                                                                      gTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAACTCTGAAGAGAAAGCGTCCCCTcc                                               >  2:275524/1‑149 (MQ=255)                                                                                                      gTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTcc                                               >  1:475991/1‑149 (MQ=255)                                                                                                         ccGGCATCGGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGCA‑GAAGAACGGCGAGTGGGGCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACACCGACGGTAAAGAAACCAGCGCCCAATCGGCACAGAACACGTCCACTCCgtc                                            >  2:569460/1‑147 (MQ=255)                                                                                                         ccGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTTTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGGAAAGAAACCGGCGCCAAATCTGCAGGGAAAGGGTCCACCCCGgc                                            >  2:569473/1‑149 (MQ=255)                                                                                                          cGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGgcg                                           >  1:427036/1‑149 (MQ=255)                                                                                                          cGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGgcg                                           >  1:611214/1‑149 (MQ=255)                                                                                                           ggCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGgcgc                                          >  1:602073/1‑149 (MQ=255)                                                                                                                   cagACGGCATTCTTGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCCCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCCGCGCCAAATCTGAAGCGCAAGCGTCCACTCCGGCGCAACGCcag                                  >  2:215384/1‑149 (MQ=255)                                                                                                                   cagACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAg                                                                                                      >  1:537537/1‑81 (MQ=255)                                                                                                                   cagACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAg                                                                                                      <  2:537537/81‑1 (MQ=255)                                                                                                                     gACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGAcagcg                                >  2:467425/1‑148 (MQ=255)                                                                                                                       cGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCCCCTGCGTGACGGCAACAGCGCCGGTAAAGAAACCAGCACCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAgg                              <  2:246673/149‑1 (MQ=255)                                                                                                                        ggCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAg                                                                                                      >  1:481652/1‑76 (MQ=255)                                                                                                                        ggCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAg                                                                                                      <  2:481652/76‑1 (MQ=255)                                                                                                                             tCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGtctc                        >  2:307686/1‑149 (MQ=255)                                                                                                                               tGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGTAGGCGTCTCTg                      >  2:347674/1‑149 (MQ=255)                                                                                                                                    gCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGACTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGAaga                 <  2:225593/149‑1 (MQ=255)                                                                                                                                             gAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGAAGAGCAAaacaa        >  1:253322/1‑149 (MQ=255)                                                                                                                                                   gAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGAAGAGCAAAACAACGATGc  <  2:418190/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TAGCCGATGCCACCGTCGCAACCTGGCATAAAAAACCCGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAGACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGG‑TAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGCAACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGAAGAGCAAAACAACGATGC  >  NC_000913_3_hsa_tpiA/761565‑761859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.