New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 229512067 (1.130)5 (0.090) 5/268 7.5 7.3% coding (261/480 nt) ccmE periplasmic heme chaperone
?NC_000913_3_hsa_tpiA = 2295133 62 (1.100)coding (248/480 nt) ccmE periplasmic heme chaperone
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGGCGTATAGTTTTCATCGTGTTTCGCCAGCACTTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/2294972‑2295120
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccttcaCCATTTACGATGCTGAAGGCTCAGTGGATGTCTCTTACGAAGGCATTTTGCCGGATCTGTTCCGTGAAGGGCAGGGCGTTGTGGTGCAGGGCGAACTGGAAAAAGGCAATCATATCCTCGCGAAAGAAGTGCTGGCGAAACACGA  <  NC_000913_3_hsa_tpiA/2295133‑2294989
                                                                                                                                                                                                                                                                                                      
GCGGCGTATAGTTTTCATCTTGTTTCGCCAGCACTTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCA                                                                                                                                                   <  2:999512/149‑1
GCGGCGTATAGTTTTCATCGTGTTTCGCCAGCACTTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCA                                                                                                                                                   <  2:913018/149‑1
                 TCGTGTTTCGCCAGCACTTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCACCATTTACGATGCTGAA                                                                                                                                  <  1:515566/149‑1
                                                                          TGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCACCATTTACGATGCTGA                                                                                                                                   >  1:527507/1‑91
                                                                          TGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCACCATTTACGATGCTGA                                                                                                                                   <  2:527507/91‑1
                                                                                                                        CTTCGTAAGAGACATCCACTGAGCCTTCACCATTTACGATGCTGAAGGCTCAGTGGATGTCTCT                                                                                                                <  1:240594/64‑1
                                                                                                                        CTTCGTAAGAGACATCCACTGAGCCTTCACCATTTACGATGCTGAAGGCTCAGTGGATGTCTCT                                                                                                                >  2:240594/1‑64
                                                                                                                                                 TTCACCATTTACGATGCTGAAGGCTCAGTGGATGTCTCTTACGAAGGCATTTTGCCGGATCTGTTCCGTGAAGGGCAGGGCGTTGTGGTGCAGGGCGAACTGGAAAAAGGCAATCATATCCTCGCGAAAGAAGTGCTGGCGAAGCACGA  >  1:289134/1‑149
                                                                                                                                                                                                                                                                                                      
GCGGCGTATAGTTTTCATCGTGTTTCGCCAGCACTTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/2294972‑2295120
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccttcaCCATTTACGATGCTGAAGGCTCAGTGGATGTCTCTTACGAAGGCATTTTGCCGGATCTGTTCCGTGAAGGGCAGGGCGTTGTGGTGCAGGGCGAACTGGAAAAAGGCAATCATATCCTCGCGAAAGAAGTGCTGGCGAAACACGA  <  NC_000913_3_hsa_tpiA/2295133‑2294989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.