Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,296,0420CT35.1% 77.7 / 77.8 95intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (31/30);  new base T (19/14);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCT  >  NC_000913_3_hsa_tpiA/4295894‑4296190
                                                                                                                                                    |                                                                                                                                                    
gtgggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                      <  1:325274/146‑1 (MQ=17)
   ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                                                                   >  1:363136/1‑149 (MQ=17)
   ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                                                                   <  1:946101/149‑1 (MQ=17)
   ggATAAGGCGTTGATGCCGCACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                                                                   <  1:569702/149‑1 (MQ=17)
    gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTTGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                                  >  1:895035/1‑149 (MQ=17)
    gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                                  <  1:625764/149‑1 (MQ=17)
     aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACCGCCTg                                                                                                                                                 >  1:720290/1‑149 (MQ=17)
     aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCCCATCCGACATCAACGCCTg                                                                                                                                                 >  2:937645/1‑149 (MQ=17)
        aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                              <  2:840358/149‑1 (MQ=32)
         ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTCTGTAGGTCGTAGAAGGGGGTTACGCCGCATCCGACTTCCACGCCTGGTGc                                                                                                                                             >  2:631754/1‑149 (MQ=11)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                          >  1:1003298/1‑149 (MQ=17)
                  gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCTGCACCCGACATCAACGCCTGATGCGACGCTTGc                                                                                                                                    <  1:1027387/149‑1 (MQ=18)
                   ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                   >  2:545962/1‑149 (MQ=21)
                   ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGAGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTAcc                                                                                                                                   >  2:534034/1‑149 (MQ=9)
                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                                                                  >  2:14407/1‑149 (MQ=25)
                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                                                                  >  1:787269/1‑149 (MQ=25)
                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                                                                  >  1:613565/1‑149 (MQ=25)
                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                                                                  >  1:584404/1‑149 (MQ=21)
                     ccaacagACAACAACGCCAGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCCGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCAACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                                 <  2:814275/143‑1 (MQ=11)
                      cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCGGATGCGAGGCTTGCcgca                                                                                                                                >  1:175224/1‑148 (MQ=11)
                       gaccGACACCAACCCTTGATCCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGAAATAGCCTGATTTCGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                               <  2:970422/147‑1 (MQ=11)
                        tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                                                                              >  1:597508/1‑149 (MQ=38)
                         ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCt                                                                                                                             >  1:647898/1‑149 (MQ=25)
                          cGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATAAATGCCTGATGCGACGCTTGCCGCGGCtt                                                                                                                            >  1:519140/1‑149 (MQ=11)
                          cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                            >  1:226604/1‑149 (MQ=25)
                          cGAAATCCACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCTCtt                                                                                                                            >  2:272903/1‑149 (MQ=11)
                           gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                           >  1:898972/1‑149 (MQ=25)
                           gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCTCGTCTTa                                                                                                                           >  1:534550/1‑149 (MQ=37)
                           gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAAGCCCTGATTTAGCGTGTTTTTGTAGGTCGCATTAGACGTTTACGCCGCATCCGACACCAATGCCTGCTGCGACGCCTGCCCCCTCTTa                                                                                                                           >  1:193860/1‑149 (MQ=11)
                            gcATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                          <  1:410863/148‑1 (MQ=19)
                            gcATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAGGCTTGCCGCGTCTTAt                                                                                                                          <  1:904668/148‑1 (MQ=255)
                            aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTACCGCGTCTTAt                                                                                                                          <  1:534034/149‑1 (MQ=12)
                            aCATCAAAGCCCGACGCGACGCATACCGCGTCTTATCAGGCTGACGCCAGACAGCGCAATAGCCTAATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTACGCCGAGCACTACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                          <  2:440649/149‑1 (MQ=11)
                             cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                         >  1:1037415/1‑149 (MQ=255)
                              agcAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                        <  1:630672/147‑1 (MQ=39)
                              acaaaCGCCTGGGGCTACGCGTAACGCGTGTTATCAAGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTGAGGTCGGATAAGTCTTTTACGCCGCATCCGATATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                        <  2:149122/146‑1 (MQ=11)
                              aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGTCCCAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                        <  1:242208/149‑1 (MQ=11)
                              aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                        >  2:665493/1‑149 (MQ=21)
                               tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                       <  1:14407/149‑1 (MQ=21)
                               tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                       <  2:324268/149‑1 (MQ=39)
                                 caCGCCGGAGGTGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     <  1:687346/148‑1 (MQ=11)
                                 aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     >  2:259719/1‑149 (MQ=21)
                                 aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     <  2:613565/149‑1 (MQ=21)
                                   cGCCTGTTGCGACGCTTAACGCGTCGTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                   <  1:916385/149‑1 (MQ=255)
                                          agcgcCGCTTAACCCGTCTTATCAGGCTTACGCAATACAGCGCAAAACCCTGATTTAGCGAGATTTCGTAGGCCGGACAAGCCGTTCACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatcttaa                                                                                                            <  2:752107/148‑7 (MQ=11)
                                           aagACGCGTTAAGCGTCGCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa                                                                                                           <  1:700366/147‑1 (MQ=14)
                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                           <  1:869511/115‑1 (MQ=33)
                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                           >  2:869511/1‑115 (MQ=32)
                                                       gcgTCTTATCAGGCCTACGCCGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGCCAGCGCATTAGc                                                                                               >  2:914187/1‑149 (MQ=255)
                                                         gTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTTATTTAGCATGATTTTGTAGGTCTTACAAGGCGTTTACGCCCCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                             <  1:701326/149‑1 (MQ=11)
                                                         gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACGGCGCAGTAGCCt                                                                                             >  2:123936/1‑149 (MQ=255)
                                                          gcTTAGCAGGCCTAAGCCATGCAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTATGAAAAGGCGTTAACGCCGCATCAGGTTTCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCGTg                                                                                            <  2:524371/148‑1 (MQ=11)
                                                              aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtct                                                                                        >  1:928685/1‑147 (MQ=38)
                                                                   gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                       <  2:235421/113‑1 (MQ=255)
                                                                   gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                       >  1:235421/1‑113 (MQ=40)
                                                                   gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCATTAGCCTGCtttctcgt                                                                                   >  2:1005009/1‑144 (MQ=255)
                                                                   gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt                                                                                   >  1:71631/1‑149 (MQ=39)
                                                                    ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                                         >  2:425104/1‑142 (MQ=38)
                                                                    ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                                         <  1:425104/142‑1 (MQ=39)
                                                                              aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                                                                    <  1:830403/73‑1 (MQ=25)
                                                                                      atgcgcTGATTTAGGGTTATTTTGTGGGTGGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                                                <  1:902655/144‑1 (MQ=11)
                                                                                       tggCCTGACTGAGGGTGATTTTGTGAGGCTGATCAGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                               <  2:134293/147‑1 (MQ=11)
                                                                                       tAGCCTGACTGCGCGTCATTATGTAGGGCGTGTCAGGCGTTTACGCCGCGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                               <  2:134207/149‑1 (MQ=11)
                                                                                           cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     <  2:44439/91‑1 (MQ=255)
                                                                                           cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     >  1:44439/1‑91 (MQ=255)
                                                                                               tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     <  1:362391/87‑1 (MQ=255)
                                                                                               tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                     >  2:362391/1‑87 (MQ=255)
                                                                                                       gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCATGATTTTGTGTGTCGGATCAGGCGTTCATGCTGTATc                                               >  2:678260/1‑149 (MQ=11)
                                                                                                         ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                               <  1:287866/51‑1 (MQ=255)
                                                                                                         ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                               >  2:287866/1‑51 (MQ=255)
                                                                                                         ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                               >  2:291418/1‑51 (MQ=255)
                                                                                                         ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                               <  1:291418/51‑1 (MQ=255)
                                                                                                          tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa                                            <  1:742619/149‑1 (MQ=38)
                                                                                                              tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                               <  1:58492/62‑1 (MQ=255)
                                                                                                              tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                               >  2:58492/1‑62 (MQ=255)
                                                                                                                  tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                                 >  2:237035/1‑88 (MQ=18)
                                                                                                                   cGGATCAGGCGTTGACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGc                                   <  1:387272/149‑1 (MQ=255)
                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                          <  1:522887/60‑1 (MQ=255)
                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                          >  2:522887/1‑60 (MQ=255)
                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                                     <  2:355725/129‑1 (MQ=40)
                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                                     >  1:355725/1‑129 (MQ=40)
                                                                                                                       tAAGGCTTTTACGCCCCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGa                               <  1:142252/149‑1 (MQ=255)
                                                                                                                           gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                           >  2:946101/1‑149 (MQ=38)
                                                                                                                              tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa                                                                                 <  2:946046/92‑1 (MQ=255)
                                                                                                                              tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa                                                                                 >  1:946046/1‑92 (MQ=255)
                                                                                                                              tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCCACGCCTGATGCGACg                        >  2:607254/1‑149 (MQ=255)
                                                                                                                                   gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa                                          >  1:843435/1‑126 (MQ=37)
                                                                                                                                   gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa                                          <  2:843435/126‑1 (MQ=37)
                                                                                                                                   gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCttca                   >  1:107506/1‑147 (MQ=21)
                                                                                                                                   cgcgcATCCGAAATCAACGCCTGAGGGGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                   <  1:694857/147‑1 (MQ=255)
                                                                                                                                    ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGCACACAGTGCCGCATCCTCCATCAACGCCTGATGCGATGCTTAAc                  >  1:287334/1‑149 (MQ=11)
                                                                                                                                           cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTACCGCGTCtt           >  1:559432/1‑149 (MQ=25)
                                                                                                                                            gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa          >  2:592215/1‑149 (MQ=35)
                                                                                                                                            gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa          <  2:71631/149‑1 (MQ=35)
                                                                                                                                               aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTAATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCAGCCACGCCTGATGCGACGCTTAACCCGTCTTATCa       >  1:752107/1‑149 (MQ=11)
                                                                                                                                                tCAGCGCCTGATGCGACGCTTGCCGCGTGTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg      <  1:784095/149‑1 (MQ=9)
                                                                                                                                                    cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCt  >  1:733714/1‑149 (MQ=11)
                                                                                                                                                    |                                                                                                                                                    
GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCT  >  NC_000913_3_hsa_tpiA/4295894‑4296190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: