Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,111,662 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,111,6620GA96.3% 159.9 / NA 55L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/20);  minor base T (0/2);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATC  >  NC_000913_3_hsa_tpiA/4111523‑4111807
                                                                                                                                           |                                                                                                                                                  
aGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTc                                                                                                                                           <  2:556293/149‑1 (MQ=255)
   tGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGcc                                                                                                                                        <  1:790319/149‑1 (MQ=255)
       cgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTg                                                                                                                                    >  2:710119/1‑149 (MQ=255)
         ccccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTc                                                                                                                                  <  1:386940/149‑1 (MQ=255)
            cggaGGGCAGGAGGTCTGTGAAACAGTATAGGGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTTTTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                               <  2:755233/146‑1 (MQ=255)
            cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                               >  1:27894/1‑149 (MQ=255)
            cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                               >  2:159026/1‑149 (MQ=255)
             gCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgcg                                                                                                                              >  2:400414/1‑149 (MQ=255)
                 ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacac                                                                                                                          >  2:157382/1‑148 (MQ=255)
                      cagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATaa                                                                                                                     >  1:634022/1‑149 (MQ=255)
                      aaagTCGGTGAAACAGTACAGGGATTCATCGGCTCAAAGGGTTTGCTTGTTGTCATTTATTCAAACCTGCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGCAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATaa                                                                                                                     <  2:603682/146‑1 (MQ=255)
                          tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                                 <  2:507653/149‑1 (MQ=255)
                            tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                               >  1:57428/1‑149 (MQ=255)
                              tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATGTATTCAAACCTTCAAGCGGTTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGGAGGTCAACCCCTTTTAGCCGTTGTCCGCAACAACATAACGCGGTga                                                                                                             >  1:622170/1‑148 (MQ=255)
                                aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCa                                                                                                            <  1:551063/148‑1 (MQ=255)
                                aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCa                                                                                                            >  2:551063/1‑148 (MQ=255)
                                aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAc                                                                                                           <  1:400414/149‑1 (MQ=255)
                                  aCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCt                                                                                                         >  1:623215/1‑149 (MQ=255)
                                        taGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc                                                                                                   <  2:27894/149‑1 (MQ=255)
                                         agagATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTATTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCAGCGACAACATAACGCAGTACACCTTCTGtct                                                                                                  >  2:380586/1‑149 (MQ=255)
                                           agaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCCGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCAGTTGTCCGCGACAACATAACGCAGAGCACGTTCTGTCTCg                                                                                                >  1:551848/1‑149 (MQ=255)
                                                           aGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACgtttgt                                                                               <  1:653039/149‑1 (MQ=255)
                                                              ctttgctttTTGTCATTTATTCAAATCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCACCAGCGAAGGTCAACCCCATTTCGCCGTTGTCCGCGACGACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGtttt                                                                            <  1:936722/149‑1 (MQ=255)
                                                                ttgctttTTGTCATTTATTCAAACCGTCCCGCAATTCAGATCGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCTCCGTTGTCCGCGACAACAGACCGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTc                                                                          <  1:769680/149‑1 (MQ=255)
                                                                   ctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGCCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTcccc                                                                       >  1:788994/1‑149 (MQ=255)
                                                                            aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACgttt                                                                                 <  1:589785/130‑1 (MQ=255)
                                                                            aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACgttt                                                                                 >  2:589785/1‑130 (MQ=255)
                                                                             tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTCAT‑ACCGTTTGTTTTTCCCCGCCGTttgta                                                             >  1:830473/1‑149 (MQ=255)
                                                                             tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTtggta                                                             >  1:317135/1‑149 (MQ=255)
                                                                              ttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTcccc                                                                       >  2:346789/1‑138 (MQ=255)
                                                                              ttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTcccc                                                                       <  1:346789/138‑1 (MQ=255)
                                                                                 ttCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCTTTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                         <  2:623215/149‑1 (MQ=255)
                                                                                 ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATAGGTACAACAGCGAAGGAAAACCCCGTGGCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTGTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                         >  1:149489/1‑149 (MQ=255)
                                                                                     aaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTACT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGc                                                     >  1:285428/1‑149 (MQ=255)
                                                                                       ctgtCAAGGGATTCAGATAGCTCCGGCTTAATCGTTTCATCAGCGAAGTTTACTCCATTTTCGCCGTTGTCCGCGACCACCTAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAg                                                   <  2:265331/146‑1 (MQ=255)
                                                                                         ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                 >  1:768707/1‑149 (MQ=255)
                                                                                          tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCTTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttt                                                >  2:693038/1‑149 (MQ=255)
                                                                                             aGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGT‑TCGGCGCGTTGAACGATTGTTTTTCGCCGCCGTTAGTAGCGACTGCAGtttttt                                             >  1:1013554/1‑149 (MQ=255)
                                                                                              gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTtagt                                                              <  2:330012/131‑1 (MQ=255)
                                                                                              gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTtagt                                                              >  1:330012/1‑131 (MQ=255)
                                                                                                     aGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCttt                                     >  1:91428/1‑149 (MQ=255)
                                                                                                      gATAGGGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtg                                    >  2:402742/1‑149 (MQ=255)
                                                                                                         aGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCACGCCGTTGGTGGCGACTGCAGTTTTTTCTGGGtttatcg                                 >  2:454353/1‑145 (MQ=255)
                                                                                                                gCTTAATCCGTTCAACAGCGAAGGTCCACCCCTTTCCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc                          <  1:380586/149‑1 (MQ=255)
                                                                                                                 cTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                         >  2:151414/1‑149 (MQ=255)
                                                                                                                    aaTCGGTCGAATAGGGATGGACAACCCCTTTTCTCTGTTGTCCGGGCAAACATAACGCAGTTCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa                      <  2:325957/149‑1 (MQ=255)
                                                                                                                     aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                     >  1:731585/1‑149 (MQ=255)
                                                                                                                      tCGGTTCAACAGCGAAGGTCATCCCCTTTTCGCCGTTGTCCGCGACCACATGTCGCAGTCCTCCTTCTGTCTCGGTGTAAT‑CAGGTTTGTTTTTCCCCGCCGTTAGTCGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAAt                    <  2:984677/149‑1 (MQ=255)
                                                                                                                      gtggTTCAACAGCGAAGGTCATCCCCTTTTCGCCGTTGTCCGCGGCAACATCACGCAGTGCTCCTTCTGTCTCGGTGTAAT‑AATGTTTGTTTTTCCCCGCCGTTAGTCGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAAt                    <  2:984679/147‑1 (MQ=255)
                                                                                                                         gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGGGACCATATGCGCCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                 <  1:660352/149‑1 (MQ=255)
                                                                                                                              aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑ACCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTT‑TGGCTTTGTGTTTTGGTCATTAATGGGGTGAgt           >  1:536676/1‑149 (MQ=255)
                                                                                                                              aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg            >  2:809548/1‑149 (MQ=255)
                                                                                                                               cAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGCTCATTAATGGGGTGAgt           >  2:138579/1‑149 (MQ=255)
                                                                                                                                   gAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGATTTGGTCATTAATGGGGTGAGTGTGc       >  1:547353/1‑149 (MQ=255)
                                                                                                                                        tCAACCCCTTTTCGCCGTTGTCCTCGACAAAATAACGCAGTGCCCCTTCTGTCTCGGTGTAAT‑AACGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc  <  2:768707/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
AGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT‑AACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATC  >  NC_000913_3_hsa_tpiA/4111523‑4111807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑