New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 302268949 (1.030)4 (0.090) 4/270 6.4 8.0% coding (1374/2871 nt) xdhD putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
?NC_000913_3_hsa_tpiA = 3022708 45 (0.980)coding (1393/2871 nt) xdhD putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGA  >  NC_000913_3_hsa_tpiA/3022542‑3022835
                                                                                                                                                   |                                                                                                                                                  
ccACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTCAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGGGCGCCt                                                                                                                                                   >  2:749541/1‑149 (MQ=255)
 cACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCACGTGGGCATGAAGCAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAAAAGTGTGCGACTg                                                                                                                                                  >  1:477186/1‑149 (MQ=255)
       tCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAAc                                                                                                                                            >  2:293198/1‑149 (MQ=255)
           tccACGTACCATGGCACGTACGCCGCCAGGTCGCGCGCTCCGTGGGTATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACACGACATACTGCTGCAAGAAGTGTGCGCCTGGGCCACCTGc                                                                                                                                        <  2:310449/148‑1 (MQ=255)
            ccAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCg                                                                                                                                       >  2:727161/1‑149 (MQ=255)
                    cATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCggg                                                                                                                               >  2:210708/1‑149 (MQ=255)
                         cACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTcc                                                                                                                          <  2:682103/149‑1 (MQ=255)
                          aCTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAATCAGGATATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCg                                                                                                                         <  1:793098/149‑1 (MQ=255)
                                         tCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGGACTGTGCCGCTac                                                                                                          >  1:473850/1‑149 (MQ=255)
                                              cgcCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCg                                                                                                     <  1:210708/149‑1 (MQ=255)
                                                  tCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGGGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTgaa                                                                                                 <  1:360102/149‑1 (MQ=255)
                                                     tGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTaca                                                                                                         >  2:355942/1‑138 (MQ=255)
                                                     tGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTaca                                                                                                         <  1:355942/138‑1 (MQ=255)
                                                       ggCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAagaaga                                                                                            <  1:727161/149‑1 (MQ=255)
                                                        gCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAagag                                                                                           >  1:59036/1‑149 (MQ=255)
                                                         cATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTtaagaaacgt                                                                                          >  2:619802/1‑145 (MQ=255)
                                                          aTGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGtt                                                                                         <  1:366385/149‑1 (MQ=255)
                                                          aTGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGtt                                                                                         >  2:695195/1‑149 (MQ=255)
                                                              aaCAGCATAAAGGTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCATTACGTGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTAtt                                                                                     <  1:409410/149‑1 (MQ=255)
                                                                     tAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAaca                                                                              >  1:222959/1‑149 (MQ=255)
                                                                           tCATGTCATTAAAGAGCGAGTTGGCGGCGGTGTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCtctc                                                                        <  1:247093/149‑1 (MQ=255)
                                                                              tGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCACTCGTc                                                                     >  1:175459/1‑149 (MQ=255)
                                                                              ggTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTc                                                                     <  1:15054/148‑1 (MQ=255)
                                                                               gTCATTAAAGAGCGAGTTGGCGGCGGTTTTGTTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCgg                                                                                                                        <  1:374513/97‑1 (MQ=255)
                                                                               gTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCgg                                                                                                                        >  2:374513/1‑97 (MQ=255)
                                                                                  aTTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGt                                                                 >  1:136614/1‑149 (MQ=255)
                                                                                    tAAAGAGCGAGTTGGCGGCGGTTTTTGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTcg                                                               <  1:293198/149‑1 (MQ=255)
                                                                                       agagCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAaca                                                                              <  1:387493/131‑1 (MQ=255)
                                                                                       agagCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAaca                                                                              >  2:387493/1‑131 (MQ=255)
                                                                                         agCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGaaa                                                          <  1:362845/149‑1 (MQ=255)
                                                                                               ttGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCAcc                                                    >  2:775495/1‑149 (MQ=255)
                                                                                                    ggcggTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGAc                                                                                                                                   >  1:295362/1‑65 (MQ=255)
                                                                                                    ggcggTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGAc                                                                                                                                   <  2:295362/65‑1 (MQ=255)
                                                                                                                tCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCAGTCGTGTTCCGCCACACCCGTGAAGAAGAGTTTATTCCTAACACCCCTCGTCAACTCGCCAAAATCACAGCCAAACTCAGAGCGaa                                   >  2:741366/1‑149 (MQ=255)
                                                                                                                   aaaCAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTa                                                                                                                      <  1:12602/63‑1 (MQ=255)
                                                                                                                   aaaCAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTa                                                                                                                      >  2:12602/1‑63 (MQ=255)
                                                                                                                    aaCAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGGGCGaaaaaa                               >  2:192145/1‑149 (MQ=255)
                                                                                                                         gACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATgg                          >  2:318369/1‑149 (MQ=255)
                                                                                                                            aTCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAACAAGATGGTCg                       >  2:650567/1‑149 (MQ=255)
                                                                                                                            aTCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTAACGTCGCGAAAGTCACCGGCAAACTGGGAGCGAAAAAAGATGGTCg                       >  1:703464/1‑149 (MQ=255)
                                                                                                                               ctgctgGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGGGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCt                    <  2:59036/149‑1 (MQ=255)
                                                                                                                                  ctgGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGAc                 >  1:468168/1‑149 (MQ=255)
                                                                                                                                     gaagaaGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCCCTCTCATGCGGGGTGCGAAAAAAGATGGTCGCCTGACGGc              >  1:232987/1‑149 (MQ=255)
                                                                                                                                      aagaagTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCa             <  2:222959/149‑1 (MQ=255)
                                                                                                                                      aagaagTGTGCGCCTGGGCAACCTGCGTGACCGGGCGGCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCa             <  1:252819/149‑1 (MQ=255)
                                                                                                                                            tgtgCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAg       >  1:109116/1‑149 (MQ=255)
                                                                                                                                               cggcCTGGGCAACTGGGGGGACGGGGCGTCCGGTACTGTTCCGCTACACCCTGGAAGAAGAGGTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTTACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATg    <  2:47779/147‑1 (MQ=255)
                                                                                                                                                cgcCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATgg   <  1:318369/149‑1 (MQ=255)
                                                                                                                                                 gcCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGa  >  2:524802/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCAACCTGCGTGACCGGGCGTCCGGTACTGTTCCGCTACACCCGTGAAGAAGAGTTTATTGCTAACACCTCTCGTCACGTCGCGAAAGTCACCGTCAAACTGGGAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGA  >  NC_000913_3_hsa_tpiA/3022542‑3022835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.