New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 2525906	42 (0.880)	8 (0.180)	5/258	5.7	16.5%	intergenic (+15/+24)	yfeN/yfeR	putative outer membrane protein/transcriptional regulator of yefH
	?	NC_000913_3_hsa_tpiA	= 2525909	42 (0.950)					intergenic (+18/+21)	yfeN/yfeR	putative outer membrane protein/transcriptional regulator of yefH
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCA  >  NC_000913_3_hsa_tpiA/2525767‑2526054                                                                                                                                                |                                                                                                                                                  ttGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGc                                                                                                                                             >  1:19971/1‑149 (MQ=255)       tgtgGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATg                                                                                                                                       >  2:826146/1‑149 (MQ=255)             ggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCa                                                                                                                                 <  1:125784/149‑1 (MQ=255)                      cATCACCCATTACTACGGAGATTCAGTATCGATATGCGGATGACACACTGGGCGAAGATTTTTACCAGGATGCGTTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTCCCGGG‑GCTCCCGGCAGTATAAATTCATCCTATTTc                                                                                                                        >  1:715066/1‑149 (MQ=255)                         cacacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGa                                                                                                                     <  2:19971/149‑1 (MQ=255)                         cacacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGa                                                                                                                     <  2:769244/149‑1 (MQ=255)                            acaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATAc                                                                                                                  >  2:6521/1‑149 (MQ=255)                                   tacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCAATCAAATTTAACTTCTGATTGATTACTGCCGGC‑GTTTCTGGCAGTATGAATTCTCTCTAGATCTGATACacgggtt                                                                                                           >  2:632706/1‑143 (MQ=255)                                        ggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCCCTCTATATCTGATGCAACGCATCCCCt                                                                                                      >  2:475776/1‑149 (MQ=255)                                        ggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCt                                                                                                      >  2:475873/1‑149 (MQ=255)                                                 aTCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                             <  2:470278/149‑1 (MQ=255)                                                          cggagtaCAAACGGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgc                                                                                    <  2:715066/143‑1 (MQ=255)                                                          cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACg                                                                                                              >  2:257916/1‑123 (MQ=255)                                                          cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACg                                                                                                              <  1:257916/123‑1 (MQ=255)                                                               gACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAtt                                                                               >  1:173007/1‑149 (MQ=255)                                                                    aCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                          <  1:543029/149‑1 (MQ=255)                                                                       gggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGATTTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt                                                                       <  1:826146/149‑1 (MQ=255)                                                                          cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                    >  1:677733/1‑149 (MQ=255)                                                                           gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATc                                                                                                          >  2:302495/1‑110 (MQ=255)                                                                           gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATc                                                                                                          <  1:302495/110‑1 (MQ=255)                                                                                 ttttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                          >  2:18734/1‑136 (MQ=255)                                                                                 ttttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                          <  1:18734/136‑1 (MQ=255)                                                                                   ttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGt                                                           <  1:82025/149‑1 (MQ=255)                                                                                     aCCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACaa                                                                                                                >  1:77766/1‑94 (MQ=255)                                                                                     aCCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACaa                                                                                                                <  2:77766/94‑1 (MQ=255)                                                                                         ggATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                             >  1:174065/1‑109 (MQ=255)                                                                                         ggATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                             <  2:174065/109‑1 (MQ=255)                                                                                           aTGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                   <  1:6521/149‑1 (MQ=255)                                                                                           aTGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                   <  1:396389/149‑1 (MQ=255)                                                                                                     cTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                         >  2:400139/1‑149 (MQ=255)                                                                                                           cATCAAATTTAGCTTCTGATTGATTACTGCCGGG‑GCTCCCGGCCGTGTGAATTCAATCTATATCTGATACAGCGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTATCGTACCACGTCCCCTAAAGCTTCAGCGGCGGTgg                                   >  1:491721/1‑149 (MQ=255)                                                                                                           cATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                   >  2:92273/1‑149 (MQ=255)                                                                                                           cATCAAAATTACCTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                   >  2:525103/1‑149 (MQ=255)                                                                                                                 aTTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                             <  2:677733/149‑1 (MQ=255)                                                                                                                       cTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGGGACCGGt                       >  1:79839/1‑149 (MQ=255)                                                                                                                        ttCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                      <  1:525103/149‑1 (MQ=255)                                                                                                                        ttCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                      <  1:175360/149‑1 (MQ=255)                                                                                                                          cTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttct                    <  1:400139/149‑1 (MQ=255)                                                                                                                          cTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGACTCGTACCACGTCCCATAAAGCTCCAGCGGCGGTGGAAAGTGAACGGttct                    >  2:796146/1‑149 (MQ=255)                                                                                                                           tgattgatTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACACCGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt                   <  1:187672/149‑1 (MQ=255)                                                                                                                                   tACTGCCGGGAGC‑CCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa           >  1:405198/1‑149 (MQ=255)                                                                                                                                      tGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTCCGCCCCa        >  1:372194/1‑149 (MQ=255)                                                                                                                                             gccTCCCGGCAGTCAGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCa  <  1:816799/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  TTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCA  >  NC_000913_3_hsa_tpiA/2525767‑2526054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.