New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 4168323	60 (0.960)	8 (0.140)	6/260	7.3	14.0%	intergenic (+141/‑31)	rrsB/gltT	16S ribosomal RNA of rrnB operon/tRNA‑Glu
	?	NC_000913_3_hsa_tpiA	= 4168345	43 (0.740)					intergenic (+163/‑9)	rrsB/gltT	16S ribosomal RNA of rrnB operon/tRNA‑Glu
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATG  >  NC_000913_3_hsa_tpiA/4168197‑4168491                                                                                                                                                     |                                                                                                                                                   aCTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCa                                                                                                                                                    <  1:856909/149‑1 (MQ=255)  cTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCaa                                                                                                                                                   >  1:1094476/1‑149 (MQ=255)  cTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCaa                                                                                                                                                   >  2:820886/1‑149 (MQ=255)  cTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCaa                                                                                                                                                   >  1:762432/1‑149 (MQ=255)   tttGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAAt                                                                                                                                                  >  2:919227/1‑149 (MQ=255)     tGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAAttt                                                                                                                                                >  2:884995/1‑149 (MQ=255)         gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGCTAAGCAATTTTctt                                                                                                                                            >  2:664346/1‑147 (MQ=37)         gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgt                                                                                                                                            <  1:182347/149‑1 (MQ=39)         gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgt                                                                                                                                            >  2:631015/1‑149 (MQ=38)         gTGCTCACACAGATTGTCTGATAGAAAGTGAAAACCAAGGCGTTTACGCGTTGGGAGTTAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgt                                                                                                                                            <  1:244185/149‑1 (MQ=255)               acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTcccc                                                                                                                                      >  2:880823/1‑149 (MQ=39)                cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCt                                                                                                                                     >  2:171209/1‑149 (MQ=39)                 acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCtt                                                                                                                                    >  1:119823/1‑149 (MQ=39)                 acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCtt                                                                                                                                    >  1:757487/1‑149 (MQ=39)                  caGACTGTCTGATAGAAAGTGAAAAGCAAGCCGCATACGCGTTGGGAGTGAAGCCGAAAAGAATAAAGCCGCCCACTTTCTATTCATGAAAGCTCACCCTAAACGAAAATATCCCGCAAAGAGTGATAAGCAAGTTCCGTATCCCATTc                                                                                                                                   >  2:478109/1‑149 (MQ=255)                    gATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGt                                                                                                                                 <  2:710548/149‑1 (MQ=37)                         tCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTaga                                                                                                                            <  1:602429/149‑1 (MQ=37)                         tCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTaga                                                                                                                            >  1:167885/1‑149 (MQ=39)                         tCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTaga                                                                                                                            <  1:240420/149‑1 (MQ=37)                         tCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGAGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTaga                                                                                                                            <  2:809084/149‑1 (MQ=255)                            gATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgt                                                                                                                                            <  1:678836/130‑1 (MQ=37)                            gATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgt                                                                                                                                            >  2:678836/1‑130 (MQ=38)                                gAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAg                                                                                                                     >  1:458729/1‑149 (MQ=33)                                gAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAg                                                                                                                     <  2:408273/149‑1 (MQ=32)                                 aaaGTGAAAAGCAAGGCGTTTACGCGTTGGGAGGGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACCCGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTATTACCTGCGTCTAGAGCCCCAgg                                                                                                                    >  2:175388/1‑149 (MQ=11)                                  aaGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATCAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCACCGCGGAATAAGCAGTCTTCGTTTCCCCTCAGTATAAAGGACCAGGa                                                                                                                   >  1:190961/1‑149 (MQ=11)                                  aaGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGa                                                                                                                   >  2:1836/1‑149 (MQ=32)                                     tGAAAAGCAAGGCGTTTACGCGTTGGGAGAGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGTAAAGATGACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGacac                                                                                                                >  1:1077319/1‑149 (MQ=255)                                      gAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGCATAAGGCGGGTCGCTTTGGATTAATAAAAGGACACACTACACGAAAATATGAAGCAACACGTGATAAGCAAATTTCGTGTCACCTTAGTCTACAGTCCCaagcgacc                                                                                                               >  2:419856/1‑142 (MQ=2)                                      gAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCa                                                                                                                      <  2:772310/142‑1 (MQ=32)                                      gAAAAGCAAGGCGTTTACGCGGTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCa                                                                                                                      >  1:772310/1‑142 (MQ=17)                                        aaaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGc                                                                                                             <  2:350417/149‑1 (MQ=31)                                        aaaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGc                                                                                                             >  1:892051/1‑149 (MQ=31)                                                                                                                        gaaaagatagCACGCAACGCGTGATAAGCAATTGTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                             <  2:272594/143‑1 (MQ=255)                                                                                                                        acGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCCCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                             >  2:422030/1‑149 (MQ=17)                                                                                                                          gAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata                           >  1:364786/1‑149 (MQ=32)                                                                                                                          gAAAATATCACGCAACGCGTGATAAGCAATCGTCGTATCCCCTTCGTCTAGAGGCCCAGAACACCGCCCTTTCACGGCGCTAACCGGGGATCGAATCCCCTGCGAGACGCCACTCGCCGGTTTGTGCGTGAAAGCCGCCGCCCttatta                           >  2:107632/1‑149 (MQ=11)                                                                                                                            aaaTATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtc                         <  2:892051/149‑1 (MQ=32)                                                                                                                            aaaTATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtc                         <  1:820886/149‑1 (MQ=32)                                                                                                                              atatCCCGCAACGCGTGATAAGCAATTTTCGTTTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGTCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtctc                       <  2:733623/149‑1 (MQ=12)                                                                                                                               tatCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGGTTGTGAGTGAAAGTCGCCGCCCTTAATATCTCa                      >  1:336733/1‑149 (MQ=17)                                                                                                                                 tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACCGGGGTTCGAATCCCCTAGGGGCCTCCCCTTGCTGGTTTGTGAGTGACAGTCGCCGACCTTAATATATCcaa                    >  2:163395/1‑149 (MQ=11)                                                                                                                                 tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaaa                    >  1:252138/1‑149 (MQ=32)                                                                                                                                  cACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaaaa                   <  2:861277/149‑1 (MQ=32)                                                                                                                                   aCGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAAc                  >  2:438326/1‑149 (MQ=32)                                                                                                                                   aCGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAAc                  >  1:649572/1‑149 (MQ=32)                                                                                                                                    cGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACt                 >  1:978775/1‑149 (MQ=32)                                                                                                                                     gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTc                >  2:92272/1‑149 (MQ=33)                                                                                                                                     gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTc                <  2:757487/149‑1 (MQ=34)                                                                                                                                     cgaaCGCCTGAGAAGCAATTTTTGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGTGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTc                <  2:235241/147‑1 (MQ=255)                                                                                                                                       accgcgTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCAt              <  1:276171/147‑1 (MQ=25)                                                                                                                                             tGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCgg        <  1:684279/149‑1 (MQ=40)                                                                                                                                               aTAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGt      <  1:317579/149‑1 (MQ=255)                                                                                                                                                  aGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAGGCCACTTGCTGGTTTGTGAGTGAAAGTCGCGCACCTTAATATCTCAAAGCTCATCTTCGGGTGAt   >  2:429969/1‑149 (MQ=255)                                                                                                                                                   gCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATg  >  2:566714/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATG  >  NC_000913_3_hsa_tpiA/4168197‑4168491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.