Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503	1979278	776	51 [0]	[0] 50	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	47 (0.800)	46/264	0.3	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_hsa_tpiA/1978357‑1978644                                                                                                                                                  |                                                                                                                                               aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTAAACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                             <  1:533263‑M1/149‑4 (MQ=255) aaaTGGGTATTTACACTTAACTAAGATTTTTTTTTTTTTTTCTCAACTGTACTCGTCACTACACGCACATACAACGAAAGGGGGCTGCGATTCTCAATAATGCGTGATACAGATCACACAAGACGCGCAATGACTTAACATAATTgtat                                                                                                                                             >  2:929071/1‑146 (MQ=255)   attgttcttgaCACTAGTCGAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                           <  2:825348‑M1/144‑6 (MQ=255)    tGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataag                                                                                                                                          >  2:407545‑M1/1‑143 (MQ=255)          tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                    <  1:688652‑M1/149‑13 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTTAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAGATGtataagtcatacttttgtt                                                                                                                             <  1:81835‑M1/149‑20 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                             >  1:127206‑M1/1‑130 (MQ=255)                   aTCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACGACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtttt                                                                                                                           <  1:606087‑M1/149‑22 (MQ=255)                       aacatttTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                                                                       <  1:507805‑M1/146‑26 (MQ=255)                        aGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                                                                      >  2:385698‑M1/1‑123 (MQ=255)                          aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAACTGtataagtcataattttgttttggtttta                                                                                                                    >  2:205073‑M1/1‑121 (MQ=255)                          aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                                                                    >  2:774495‑M1/1‑121 (MQ=255)                                 cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                             >  2:321211‑M1/1‑114 (MQ=255)                                   aaaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatct                                                                                                           >  2:749478‑M1/1‑112 (MQ=255)                                           gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                   <  1:777288‑M1/149‑46 (MQ=255)                                             aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                                                                 >  2:797986‑M1/1‑102 (MQ=255)                                                     tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATCtataagtcatacttttcttttgggtgtatttcaatctgttaaaaaattttttgct                                                                                         >  2:273420‑M1/1‑94 (MQ=255)                                                       gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctac                                                                                       >  2:889840‑M1/1‑92 (MQ=255)                                                        tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacg                                                                                      >  1:390692‑M1/1‑91 (MQ=255)                                                         caatacaAGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                     <  2:311988‑M1/146‑60 (MQ=255)                                                              cacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagca                                                                                >  1:850860‑M1/1‑85 (MQ=255)                                                               acgaccatacaaCGGATGAGGGCTCCGATTTTCAATAATGCGTTATGCAGATCACACAAAACACTCAATTACTTAACCTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                               <  1:734220‑M1/144‑66 (MQ=255)                                                                cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                                                              <  1:774495‑M1/149‑67 (MQ=255)                                                                     aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattacaatcgtttaaaaagtttttcgctacgctagcaagcaaaa                                                                         >  1:853983‑M1/1‑78 (MQ=255)                                                                         aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                     >  1:359132‑M1/1‑74 (MQ=255)                                                                           cGGAGGGGGGCTGCGATTTTCAATAAGGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatctcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   <  2:753174‑M1/149‑78 (MQ=255)                                                                                  gggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaat                                                            <  1:22987‑M1/149‑85 (MQ=255)                                                                                   ggCTGCGATTTTCAATAAGGCTGGATTCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaata                                                           <  1:419417‑M1/149‑86 (MQ=255)                                                                                      tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                        >  2:686883‑M1/1‑61 (MQ=255)                                                                                         gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaa                                                     >  1:890251‑M1/1‑58 (MQ=255)                                                                                               cAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggga                                               <  1:889840‑M1/149‑98 (MQ=255)                                                                                                  taatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                               <  2:147985‑M1/98‑50 (MQ=255)                                                                                                  taatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                               >  1:147985‑M1/1‑49 (MQ=255)                                                                                                   aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgtt                                           <  2:198792‑M1/149‑102 (MQ=255)                                                                                                    atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATGAATGtataagtcatacttttgttttgggtgtacttcaatctgttaaaaagttttgcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttg                                          >  2:1039383‑M1/1‑47 (MQ=255)                                                                                                        gTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                      <  1:797986‑M1/149‑107 (MQ=255)                                                                                                            tGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactattgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcaaaaaggtatgttgcgcacagt                                  >  2:467057‑M1/1‑39 (MQ=255)                                                                                                             gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgagatgtttggcacagtc                                 >  2:763025‑M1/1‑38 (MQ=255)                                                                                                               aGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggttgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatagaaatggaaattttcgtacagtcaa                               >  2:722659‑M1/1‑36 (MQ=255)                                                                                                                gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatagaaatgggatgttgcgcacagtcaaa                              >  2:726643‑M1/1‑35 (MQ=255)                                                                                                                 ataacacaaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                             <  1:749478‑M1/146‑116 (MQ=255)                                                                                                                    acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                          <  1:321211‑M1/149‑119 (MQ=255)                                                                                                                    acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                             <  1:878452‑M1/66‑36 (MQ=255)                                                                                                                    acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                             >  2:878452‑M1/1‑31 (MQ=255)                                                                                                                      acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataact                        >  2:576596‑M1/1‑29 (MQ=255)                                                                                                                          aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaacaatgaaacaggaataatcgaaattggatgttgagcacagtcaaaacaacacacc                    >  2:374531‑M1/1‑25 (MQ=255)                                                                                                                                      cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcat        >  1:865096‑M1/1‑13 (MQ=255)                                                                                                                                         aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctg     >  1:1009699‑M1/1‑10 (MQ=255)                                                                                                                                           cATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaacaatcatctgct   <  1:691968‑M1/149‑142 (MQ=255)                                                                                                                                            aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagccagcaaaaatgaaacaggaataatagaaatgggatgttgcgcacagtcagaataactcaccgtgaataatcatctgcta  >  1:548774‑M1/1‑7 (MQ=255)                                                                                                                                            aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  >  2:920862‑M1/1‑7 (MQ=255)                                                                                                                                                  |                                                                                                                                               AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_hsa_tpiA/1978357‑1978644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑