Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,111,662 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,111,6620GA97.8% 284.4 / NA 89L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (57/30);  minor base C (1/1);  total (58/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAT  >  NC_000913_3_hsa_tpiA/4111517‑4111806
                                                                                                                                                  |                                                                                                                                                
aaaGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAccc                                                                                                                                               <  1:2619/149‑1 (MQ=255)
    gAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCttt                                                                                                                                           >  2:17863/1‑149 (MQ=255)
         tGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCTTTTATTGAAACCTTCACGCGATTCAGATAGAGCCAGCTTATTGAGTTCAACAGCGAAGGTCACCCCCTCTTCGcc                                                                                                                                      >  2:879477/1‑149 (MQ=255)
         ctgctgccCTGCGGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAccc                                                                                                                                               <  1:356823/135‑1 (MQ=255)
           gccgccCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGt                                                                                                                                    >  2:725651/1‑149 (MQ=255)
            ccgccCCGCAGGGCAGCAGGTCTGTGAAAAAATCAGCGCGA‑GCATCGGCACAAAGGCTTTGCTTTTTGTCCTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTGTTCGCCGtt                                                                                                                                   >  1:540978/1‑149 (MQ=255)
              gccCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGCTTCAGATAGCGCCAGCTTCATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGt                                                                                                                                 >  1:784210/1‑149 (MQ=255)
                  cGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                             >  2:58709/1‑149 (MQ=255)
                          agcagGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACAt                                                                                                                     <  2:620823/149‑1 (MQ=255)
                           gcagGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTGTTCCGCGACAACATa                                                                                                                    >  2:215397/1‑149 (MQ=255)
                               gTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                                >  1:822302/1‑149 (MQ=255)
                                 ctttgAAACGGT‑ATAGATATTCACCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTCATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCATTGTCCGCGACCACATAACGCAg                                                                                                              <  2:630860/146‑1 (MQ=255)
                                  tgtgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTTTTTTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGGGAACGTCAACCCCTTTTCGCCGCTGTCCGCGCCACCCTAACACAGt                                                                                                             >  2:471173/1‑149 (MQ=255)
                                  tgtgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                             >  2:865851/1‑149 (MQ=255)
                                  tgtgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                             <  2:1066801/149‑1 (MQ=255)
                                   gtgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                                                                                                            <  1:945866/149‑1 (MQ=255)
                                    tgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                                <  2:903089/144‑1 (MQ=255)
                                    tgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                                >  1:903089/1‑144 (MQ=255)
                                    tgAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGc                                                                                                           <  1:590024/149‑1 (MQ=255)
                                      aaaCAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTTTTTTTTTTTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAc                                                                                                         >  2:951958/1‑149 (MQ=255)
                                      aaaCAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAGCATAACGCAGTGCAc                                                                                                         >  1:529470/1‑149 (MQ=255)
                                      aaaCAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAc                                                                                                         >  2:14838/1‑149 (MQ=255)
                                          aGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAGCCCTTTTCGCCGTTGTCCGCGacaaca                                                                                                                      >  2:633817/1‑132 (MQ=255)
                                          aGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAGCCCTTTTCGCCGTTGTCCGCGacaaca                                                                                                                      <  1:633817/132‑1 (MQ=255)
                                            t‑ataGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTg                                                                                                   >  1:585992/1‑149 (MQ=255)
                                            t‑ataGAGATTCATCGGCACAAAGGCTTTGATTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAAGTCAACCCCTTTTCGCCGTTGTCCGCCACCACATGACGCAGTGCACCTtgtg                                                                                                   >  1:697138/1‑149 (MQ=255)
                                                  agaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCg                                                                                              >  1:714088/1‑149 (MQ=255)
                                                  agaTTCAACGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCACGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCg                                                                                              <  2:685902/149‑1 (MQ=255)
                                                    aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgt                                                                                            >  1:727999/1‑149 (MQ=255)
                                                     ttCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgtg                                                                                           <  1:106880/149‑1 (MQ=255)
                                                     ttCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCATTTTCGCCGTTGTCCGCGACAACAGAACGCAGTGCACCTTCTGTCTCGgtg                                                                                           >  1:599186/1‑149 (MQ=255)
                                                             cacaAAGGCTTTGCTTTTTTTTTTTTTTTTAAACCTTCAAGCGCTTCAGATAGCGCCAGCTTAATCTGTTGAACAGCGAAGGTCAACCCCTTTTTGCCCTTGTCCGCGACACCATATCTCATTGCACCTTCTCTCTCGGTGTAATAAct                                                                                   >  2:178464/1‑148 (MQ=255)
                                                              acaAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgt                                                                                  >  2:723819/1‑149 (MQ=255)
                                                                aaaGTCTTTGCTTTTTGTCATTCTTTCCAACCTTCAAGCGATTCAAATATCGTCAGCGTAATCGGTTCAACCGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgttt                                                                                <  2:713556/149‑1 (MQ=255)
                                                                  aGGCTTTGCTTTTTTTTTTTTTTTCAAACCTTAAAGCGATTCAGATAGCGCCACCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACATCATTACTCATTCCACTCTCTGTCTCGGTGTATTACCgttttt                                                                              >  2:611780/1‑147 (MQ=255)
                                                                   ggCTATGATTTTTGTGATTTATTGGAACCTTCGGGCGATTCAGATACCGCCAGCTTAACCGGTTCAACAGCGAAGTACACCCCCTTTTCGCCGTTGTCCGCGAAAACATAACGCCGTGCACCTTCTGTCTCGGTGTAATAACgtttgtt                                                                             <  2:639459/149‑1 (MQ=255)
                                                                       ttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTTGGTGTGATAACGTTTGTTTTTc                                                                         >  1:171796/1‑149 (MQ=255)
                                                                         gctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATCACGTTTGTTTTTccc                                                                       >  2:1083905/1‑149 (MQ=255)
                                                                          ctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATGACGCAGGGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcccc                                                                      >  1:637053/1‑149 (MQ=255)
                                                                          ctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcccc                                                                      <  1:14838/149‑1 (MQ=255)
                                                                            ttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgc                                                                    >  1:1059665/1‑149 (MQ=255)
                                                                                 tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTta                                                               >  2:713649/1‑149 (MQ=255)
                                                                                 tCATTTATGCAACCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGCCAACCCCTTTTCGCCGTTGTCCGCGACAAAATAACGCAGTGCACCTTCTGTCCCGGTGTAATAACGTTTGTTTTTCCCCGCCGTta                                                               <  1:426723/149‑1 (MQ=255)
                                                                                 tCATATAGTCAAACCTTAAAGTGATTCAGATAGCGCTAGTTTAATTGGTTCAACAGCGAATGTCAACCCTTTTTCTCCGTCGTCCGCGACAACATAACGCAGTGCACCTTCTGTATTTGGGCAATAACGTTTGTTTTTCCCCGCCGTta                                                               <  2:1060993/149‑1 (MQ=255)
                                                                                  cATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTACTAACGTTTGTTTTTCCCCGCCGTtag                                                              >  2:606710/1‑149 (MQ=255)
                                                                                  cATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTCTAATAACGTTTGTTTTTCCCCGCCGTtag                                                              >  2:608876/1‑149 (MQ=255)
                                                                                   aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                             >  2:394282/1‑149 (MQ=255)
                                                                                      tATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCTCCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGc                                                          <  1:865851/149‑1 (MQ=255)
                                                                                        ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                        <  1:17863/149‑1 (MQ=255)
                                                                                        ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCCTtagtacggc                                                        >  2:45443/1‑145 (MQ=255)
                                                                                           aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTg                                                     >  2:359647/1‑149 (MQ=255)
                                                                                           aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGATTGTTTTTCCCCCCCGATATTAGCGCCTg                                                     >  2:574352/1‑149 (MQ=255)
                                                                                             aCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGATCGAACGGGTGTTTTTCCCCGCCGTTAGTAGCGACTGCa                                                   >  2:506381/1‑149 (MQ=255)
                                                                                              ccTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCAGTTAGTAGCGACTGCAg                                                  >  1:1066061/1‑149 (MQ=255)
                                                                                                ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc                                                                                                     >  2:19557/1‑96 (MQ=255)
                                                                                                ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc                                                                                                     <  1:19557/96‑1 (MQ=255)
                                                                                                ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCGGtt                                                >  2:384272/1‑149 (MQ=255)
                                                                                                ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                >  1:688473/1‑149 (MQ=255)
                                                                                                ttCAAGAGCGTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                >  2:11374/1‑149 (MQ=255)
                                                                                                    aGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttttt                                            >  2:40644/1‑149 (MQ=255)
                                                                                                     gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGGCTGCAGTTTTTTc                                           >  2:610540/1‑149 (MQ=255)
                                                                                                     gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                           >  1:267238/1‑149 (MQ=255)
                                                                                                     gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                           <  1:58709/149‑1 (MQ=255)
                                                                                                         ttCAGATAGGTCCAGCTTAATCGGTTCAACGGCGAAGGTAAACCCCTGTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                       <  2:1066061/149‑1 (MQ=255)
                                                                                                         ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                       >  1:137858/1‑149 (MQ=255)
                                                                                                         ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                       >  1:34842/1‑149 (MQ=255)
                                                                                                              aTAGCGCCAGCTTAATCGGTTCAACAGCGCAGGTCAACCCCTTTTCGCTTTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCCCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                  <  2:1059665/149‑1 (MQ=255)
                                                                                                              aTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTCTCCTACCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                  <  1:1085398/149‑1 (MQ=255)
                                                                                                                   gcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGGCAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                             >  1:590290/1‑149 (MQ=255)
                                                                                                                    ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                            >  1:173674/1‑149 (MQ=255)
                                                                                                                    ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTCTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                            <  1:723078/149‑1 (MQ=255)
                                                                                                                     cAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTgg                           >  2:468688/1‑149 (MQ=255)
                                                                                                                       gCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCtt                                                                                                      >  2:5069/1‑72 (MQ=255)
                                                                                                                       gCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCtt                                                                                                      <  1:5069/72‑1 (MQ=255)
                                                                                                                       gCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTTGGTTTTGGTc                         >  1:567890/1‑149 (MQ=255)
                                                                                                                        cTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                            >  2:87904/1‑145 (MQ=255)
                                                                                                                        cTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                            <  1:87904/145‑1 (MQ=255)
                                                                                                                         ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                       <  1:129946/149‑1 (MQ=255)
                                                                                                                            aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTgg                                        >  2:576658/1‑129 (MQ=255)
                                                                                                                            aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTgg                                        <  1:576658/129‑1 (MQ=255)
                                                                                                                            aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTGTCTGGAGTTGTGTTTTGGTCATTaa                    >  2:160852/1‑149 (MQ=255)
                                                                                                                            aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACATTATGGCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGGCTTCGGTTTTTGCTGGCTTGGTGTTTTGGGCAGTat                    >  1:371014/1‑148 (MQ=255)
                                                                                                                             tCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGCTTTGGTCATTaac                   >  2:64619/1‑148 (MQ=255)
                                                                                                                                gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                <  1:394282/149‑1 (MQ=255)
                                                                                                                                       aGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgtg         >  1:867482/1‑149 (MQ=255)
                                                                                                                                         cGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGtgtg       >  1:210407/1‑149 (MQ=255)
                                                                                                                                            aGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTCGTCATTAATGGGGTGAGTGTGCgg    <  1:951958/149‑1 (MQ=255)
                                                                                                                                              gTCAACCCCTTTTTTCCGTTTTCCGCGACAACATAACGCAGTGCACCTTCTGTCACGGTGTAATAACGTTTGGTTTTCCCCTCCGTTAGTCGCCACTCCCGTTTTTTCTTGTTTTGTGTTTTGGTCATTAATTGGGTGAgtgttctgct  >  2:19163/1‑143 (MQ=255)
                                                                                                                                              gTCAACCCCTTTTCGCCGTTGTCCGCGACAACAAAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt  <  1:72384/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGT‑ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAT  >  NC_000913_3_hsa_tpiA/4111517‑4111806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑