Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	3,156,591	0	T	G	20.6%	116.5 / 34.4	68	intergenic (+73/‑32)	yqhD/dkgA	aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base T (21/33);  new base G (7/7);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAG  >  NC_000913_3_hsa_tpiA/3156446‑3156737                                                                                                                                                  |                                                                                                                                                   cATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGCCTTAAGTTCACACCCCCTCACCGGAACCTGCt                                                                                                                                                 >  1:951275/1‑149 (MQ=255)     aCCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGGGCCTGCTCCgg                                                                                                                                             >  1:110122/1‑149 (MQ=255)      cccACCTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                                                                                                            <  1:168783/149‑1 (MQ=255)       ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCCCCgctc                                                                                                                                           >  2:161659/1‑146 (MQ=255)       ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGACGCACGGTACGCTTTTTAGGCCTAAAACTGTCGTTTGCGGACATTTCGTCCAGACTTAAGTTCACAACACCCCACCGTACCCTGCTCCggag                                                                                                                                           >  2:385095/1‑147 (MQ=255)        cAACGGGGCGAAGATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAGGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGa                                                                                                                                          <  2:201833/149‑1 (MQ=255)         aaCTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                                                                         <  1:757833/149‑1 (MQ=255)         aaCTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGTGAg                                                                                                                                         <  1:1035736/149‑1 (MQ=255)            tGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTc                                                                                                                                      <  1:923802/149‑1 (MQ=255)              ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCat                                                                                                                                    <  1:301979/149‑1 (MQ=255)                    aaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAag                                                                                                                              <  1:808525/149‑1 (MQ=255)                     aTCATGACCTGACGTTGGATGTCAGCCGCCGTATATAGGAAGCCGCCCGCTAAGCTTTTTCCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagg                                                                                                                             <  1:803832/149‑1 (MQ=255)                      tCATGACAGTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                            <  1:941641/149‑1 (MQ=255)                         tGACATTACGTTGGATGTCAGCCGCCGTATATATGGAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAggagga                                                                                                                         >  2:1094962/1‑149 (MQ=255)                          cagaTTACGTTGTGTGTCCGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTCTTCCGCCGCAAACTTTCGTTTTCGGGCATTTCGTCCAGCCTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                                                                                                        <  1:538503/146‑1 (MQ=255)                               tACGTTGGATGTCAGCCGCCGTATATACGACGCGGCCATCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGGCTTAATTGCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTAt                                                                                                                   <  2:951275/149‑1 (MQ=255)                                  gTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATCTAAAGGAGGACCGAATGGc                                                                                                                >  1:1101420/1‑149 (MQ=255)                                  gTTGGATGTCAGCCGCCATATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                                                                >  2:945605/1‑149 (MQ=255)                                       aTGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTGGGGCATTGCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATGTAAAGGAGAAACGTATGGCTAATc                                                                                                           >  1:384129/1‑149 (MQ=255)                                         gTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCa                                                                                                         >  1:807631/1‑149 (MQ=255)                                          tCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggtg                                                                                                                                      >  1:135396/1‑116 (MQ=255)                                          tCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggtg                                                                                                                                      <  2:135396/119‑4 (MQ=255)                                            aGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGATGCGTTCATATAAAGGAGGAACGTATGGCTCATCCAAcc                                                                                                      >  2:656197/1‑149 (MQ=255)                                                         aCGAAGCCGCCCGCTAAGTTTTTTACTCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAc                                                                                         <  2:449885/149‑1 (MQ=255)                                                               ccgcccgcTAAGCTTTTTACGCCTCAAACTTTCTTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                   <  1:953007/149‑1 (MQ=255)                                                               ccgcccgcTAAGCTTTTTACGCCACAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                   <  2:509606/149‑1 (MQ=255)                                                                cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCat                                                                                                                                    <  2:523172/99‑1 (MQ=255)                                                                cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCat                                                                                                                                    >  1:523172/1‑99 (MQ=255)                                                                cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATgg                                                                                  <  2:302844/149‑1 (MQ=255)                                                                   ccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCaa                                                                               <  1:275216/149‑1 (MQ=255)                                                                           cTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                                                                                                        >  1:122366/1‑100 (MQ=255)                                                                           cTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                                                                                                        <  2:122366/100‑1 (MQ=255)                                                                                 aCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc                                                                 <  1:19970/149‑1 (MQ=255)                                                                                 aCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc                                                                 <  1:398351/149‑1 (MQ=255)                                                                                     cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGGGTTCATATAAAGGAAGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg                                                             >  2:1005790/1‑149 (MQ=255)                                                                                      tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                                                                <  2:468252/113‑1 (MQ=255)                                                                                      tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                                                                >  1:468252/1‑113 (MQ=255)                                                                                      tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa                                                            >  1:659001/1‑149 (MQ=255)                                                                                      tCAAACCTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGt                                                                                                                                            >  1:1035615/1‑69 (MQ=25)                                                                                      tCAAACCTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGt                                                                                                                                            <  2:1035615/69‑1 (MQ=37)                                                                                          aCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                   >  2:108164/1‑122 (MQ=255)                                                                                          aCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                   <  1:108164/122‑1 (MQ=255)                                                                                          aCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTgg                                                        <  1:161659/149‑1 (MQ=255)                                                                                                gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAAAACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCt                                                  <  1:81290/149‑1 (MQ=255)                                                                                                 ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAg                                                                                             >  2:761506/1‑105 (MQ=255)                                                                                                 ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAg                                                                                             <  1:761506/105‑1 (MQ=255)                                                                                                 ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGc                                                                                 <  2:76876/117‑1 (MQ=255)                                                                                                 ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGc                                                                                 >  1:76876/1‑117 (MQ=255)                                                                                                 ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                                                               >  1:431624/1‑135 (MQ=255)                                                                                                 ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                                                               <  2:431624/135‑1 (MQ=255)                                                                                                   ttCGGGCATTTCGTCCATACTTAAGTTCACAACATCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgc                                               <  1:462971/149‑1 (MQ=255)                                                                                                         cATTTCGTGCAGACTTAAGTTCCCAACAACTCACAGGAGACTGATCCGGTGAGTTCATATAAAGGAGGAACATATGGCTACTCCGGCGGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                         <  2:753119/149‑1 (MQ=255)                                                                                                         cATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                         <  1:114236/149‑1 (MQ=255)                                                                                                              cGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCACCCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAAt                                    <  2:309012/149‑1 (MQ=255)                                                                                                                    gACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGaa                              <  2:807631/149‑1 (MQ=255)                                                                                                                            ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTgg                                                        <  1:746969/115‑1 (MQ=255)                                                                                                                            ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTgg                                                        >  2:746969/1‑115 (MQ=255)                                                                                                                               acaacaCCTCCCCGGAGCCTGCTACGGTGAGTTCATATAAAGGAAGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGAGGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGc                   <  1:177869/149‑1 (MQ=255)                                                                                                                                 aatacCTCACCGTAGCCGGCGCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGGCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCa                 <  2:1131700/146‑1 (MQ=255)                                                                                                                                    acCTCACTGTTGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTc              <  1:547494/149‑1 (MQ=255)                                                                                                                                       tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACt                                                          >  2:437788/1‑102 (MQ=255)                                                                                                                                       tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACt                                                          <  1:437788/102‑1 (MQ=255)                                                                                                                                       tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCgaa           >  1:1088255/1‑149 (MQ=255)                                                                                                                                       tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa           >  1:463127/1‑149 (MQ=255)                                                                                                                                       tCACCGGAGCAGGCTCCGGTGAGTGCAGAGAAAGGAGGCACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCGAGCAAGTAATGAGGCAGTAATCAACGCCATTCaaa           >  1:614788/1‑149 (MQ=255)                                                                                                                                         aCCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                              >  2:686647/1‑96 (MQ=255)                                                                                                                                         aCCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                              <  1:686647/96‑1 (MQ=255)                                                                                                                                                ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGACTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAg  >  1:537513/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAG  >  NC_000913_3_hsa_tpiA/3156446‑3156737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑