BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,111,481	G→T	intergenic (+796/+116)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,111,481	0	G	T	100.0%	368.9 / NA	104	intergenic (+796/+116)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (51/53); total (51/53)

Only 100 of 104 total aligned reads displayed.

GTCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAT  >  NC_000913_3_hsa_tpiA/4111340‑4111627

gTCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCAt                                                                                                                                            >  2:565725/1‑150 (MQ=255)

gTCGATATAGGCAATAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCACACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGCAGAACTTCCTGGATGGCGCCAt                                                                                                                                            >  2:343594/1‑150 (MQ=255)

 tCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCAtt                                                                                                                                           >  3:321178/1‑150 (MQ=255)

  cGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCAttt                                                                                                                                          <  3:601311/150‑1 (MQ=255)

   gATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCAtttt                                                                                                                                         >  1:545785/1‑150 (MQ=255)

     tataGGCAGTAGGGGGAGCACAAACCGCCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTaa                                                                                                                                       <  3:764356/150‑1 (MQ=255)

     tataGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTaa                                                                                                                                       <  3:742543/150‑1 (MQ=255)

         ggCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATtct                                                                                                                                   >  1:1010596/1‑150 (MQ=255)

            aGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCa                                                                                                                                <  2:925045/150‑1 (MQ=255)

               aGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGc                                                                                                                             <  1:1229357/150‑1 (MQ=255)

               aGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTCCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGc                                                                                                                             <  4:651754/150‑1 (MQ=255)

                gggggAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCt                                                                                                                            >  2:481050/1‑150 (MQ=255)

                  gggAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTta                                                                                                                          >  1:1091685/1‑150 (MQ=255)

                   ggAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTtat                                                                                                                         >  1:256772/1‑150 (MQ=255)

                    gAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCACCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTtata                                                                                                                        >  1:953106/1‑150 (MQ=255)

                       cacaAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGc                                                                                                                     >  4:679428/1‑150 (MQ=255)

                       cacaAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGc                                                                                                                     <  2:951520/150‑1 (MQ=255)

                         caAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGGCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGa                                                                                                                   <  1:789068/150‑1 (MQ=255)

                         caAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGa                                                                                                                   <  2:183831/150‑1 (MQ=255)

                          aaaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGaa                                                                                                                  >  1:1075327/1‑150 (MQ=255)

                          aaaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGaa                                                                                                                  >  2:378286/1‑150 (MQ=255)

                              caccTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCACCGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATaaa                                                                                                              <  1:678251/150‑1 (MQ=255)

                               accTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCTCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAg                                                                                                             <  1:822020/150‑1 (MQ=255)

                                 cTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGa                                                                                                           >  2:150537/1‑150 (MQ=255)

                                      tgtCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATg                                                                                                      <  2:819791/150‑1 (MQ=255)

                                        tCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgc                                                                                                    <  2:256772/150‑1 (MQ=255)

                                         cggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGACGCACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgcc                                                                                                   >  1:664100/1‑150 (MQ=255)

                                         cggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgcc                                                                                                   <  3:343009/150‑1 (MQ=255)

                                         cggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgcc                                                                                                   <  1:927771/150‑1 (MQ=255)

                                         cggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgcc                                                                                                   <  3:65909/150‑1 (MQ=255)

                                          ggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgccg                                                                                                  >  1:489142/1‑150 (MQ=255)

                                           gccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgccgc                                                                                                 >  1:180689/1‑150 (MQ=255)

                                            ccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgccgcc                                                                                                <  1:307633/150‑1 (MQ=255)

                                               gcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCg                                                                                             <  1:951221/150‑1 (MQ=255)

                                               gcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATCACCGcccgg                                                                                             >  4:253619/1‑148 (MQ=255)

                                                cACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGc                                                                                            <  2:951242/150‑1 (MQ=255)

                                                 aCCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCa                                                                                           <  2:496567/150‑1 (MQ=255)

                                                   cTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCTAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAgg                                                                                         >  4:214664/1‑150 (MQ=255)

                                                   cTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAgg                                                                                         >  4:159253/1‑150 (MQ=255)

                                                    ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                        >  3:586426/1‑150 (MQ=255)

                                                         cgcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagca                                                                                   <  1:93027/150‑1 (MQ=255)

                                                          gcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGcagcag                                                                                  <  1:671536/150‑1 (MQ=255)

                                                            gTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGt                                                                                >  2:835401/1‑150 (MQ=255)

                                                                 gagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgtg                                                                           >  1:374736/1‑150 (MQ=255)

                                                                    tGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGc                                                                                                                     >  2:1345519/1‑105 (MQ=255)

                                                                    tGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGc                                                                                                                     <  1:1345519/105‑1 (MQ=255)

                                                                    tGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGa                                                                                                           >  1:1320685/1‑115 (MQ=255)

                                                                    tGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGa                                                                                                           <  2:1320685/115‑1 (MQ=255)

                                                                      ccGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                      <  1:378286/150‑1 (MQ=255)

                                                                      ccGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                      <  2:1091685/150‑1 (MQ=255)

                                                                         aTGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGta                                                                   >  2:511768/1‑150 (MQ=255)

                                                                             gCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                               >  1:1213080/1‑150 (MQ=255)

                                                                             gCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                               <  1:1174967/150‑1 (MQ=255)

                                                                              cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                              >  1:1380242/1‑150 (MQ=255)

                                                                              cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                              >  2:689741/1‑150 (MQ=255)

                                                                               tCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAgaga                                                             <  1:539681/150‑1 (MQ=255)

                                                                                 ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCa                                                                                                                                <  4:211643/81‑1 (MQ=255)

                                                                                 ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCa                                                                                                                                >  3:211643/1‑81 (MQ=255)

                                                                                   ccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTCAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCa                                                         <  2:803199/150‑1 (MQ=255)

                                                                                     aGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                       <  1:406689/150‑1 (MQ=255)

                                                                                        ctctGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGc                                                    <  3:273737/150‑1 (MQ=255)

                                                                                         tctGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGca                                                   <  3:794314/150‑1 (MQ=255)

                                                                                         tctGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGca                                                   <  3:830149/150‑1 (MQ=255)

                                                                                              ttCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAg                                              >  1:1073381/1‑150 (MQ=255)

                                                                                              ttCCGCCCGTTCAGCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAg                                              >  2:265869/1‑150 (MQ=255)

                                                                                                 cGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgct                                           >  3:656922/1‑150 (MQ=255)

                                                                                                  gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctt                                          >  3:439452/1‑150 (MQ=255)

                                                                                                  gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctt                                          <  1:412907/150‑1 (MQ=255)

                                                                                                   cccGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgcttt                                         <  4:571919/150‑1 (MQ=255)

                                                                                                    ccGTTCAGCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttg                                        <  2:1086562/150‑1 (MQ=255)

                                                                                                         cATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCtttt                                   <  2:545785/150‑1 (MQ=255)

                                                                                                          aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTACTTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                  >  4:331317/1‑150 (MQ=255)

                                                                                                          aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                  <  2:717068/150‑1 (MQ=255)

                                                                                                          aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                  <  2:531978/150‑1 (MQ=255)

                                                                                                          aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                  <  2:489142/150‑1 (MQ=255)

                                                                                                            cTTCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGt                                <  4:377097/150‑1 (MQ=255)

                                                                                                             ttCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTc                               >  2:516907/1‑150 (MQ=255)

                                                                                                             ttCCAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTc                               >  4:597393/1‑150 (MQ=255)

                                                                                                               ccAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAt                             <  4:568820/150‑1 (MQ=255)

                                                                                                                cAGTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAtt                            <  2:180689/150‑1 (MQ=255)

                                                                                                                  gTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTa                          >  3:827690/1‑150 (MQ=255)

                                                                                                                  gTTTCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTa                          >  1:986223/1‑150 (MQ=255)

                                                                                                                   tttCCCCCAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTAt                         >  4:495156/1‑150 (MQ=255)

                                                                                                                      cccccAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCa                      <  2:414441/150‑1 (MQ=255)

                                                                                                                        cccAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                            <  4:458179/94‑1 (MQ=255)

                                                                                                                        cccAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                            >  3:458179/1‑94 (MQ=255)

                                                                                                                        cccAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaaa                    >  1:1153449/1‑150 (MQ=255)

                                                                                                                         ccAACGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAAc                   >  2:1242199/1‑150 (MQ=255)

                                                                                                                             cgacgaACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTc               <  3:854465/150‑1 (MQ=255)

                                                                                                                             cGAAGAACTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTc               <  3:689328/150‑1 (MQ=255)

                                                                                                                              gaagaaCTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCa              >  2:1328693/1‑150 (MQ=255)

                                                                                                                               aagaaCTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCaa             <  2:1213080/150‑1 (MQ=255)

                                                                                                                                agaaCTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAg            <  1:293179/150‑1 (MQ=255)

                                                                                                                                agaaCTTCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGAGCAGCGGGTCTGTGAAACAGTGGAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAg            >  1:133147/1‑150 (MQ=255)

                                                                                                                                     ttCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAtt       >  2:415921/1‑150 (MQ=255)

                                                                                                                                      tCCTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTc      >  2:665845/1‑150 (MQ=255)

                                                                                                                                        cTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaaa                                               >  2:1080357/1‑107 (MQ=255)

                                                                                                                                        cTGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaaa                                               <  1:1080357/107‑1 (MQ=255)

                                                                                                                                         tGGATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGa   >  3:666785/1‑150 (MQ=255)

                                                                                                                                          ggATGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAt  <  1:343594/150‑1 (MQ=255)

GTCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAT  >  NC_000913_3_hsa_tpiA/4111340‑4111627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`