New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	4615228 =	46 (1.250)	3 (0.090)	3/262	NT	6.5%	coding (298/864 nt)	yjjW	putative pyruvate formate lyase activating enzyme
	?	NC_000913_3_hsa_tpiA	4615258 =	43 (1.220)					coding (268/864 nt)	yjjW	putative pyruvate formate lyase activating enzyme

AGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCC  >  NC_000913_3_hsa_tpiA/4615080‑4615375                                                                                                                                                     |                                                                                                                                                    aGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCATCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTc                                                                                                                                                     <  1:160060/149‑1 (MQ=255)   aCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATCACAAGGACTGCTTATGGGACATGGCTAATCACTTCGTCCa                                                                                                                                                   >  1:191187/1‑149 (MQ=255)     ggacgatgggacgttgcaatgactgtggggagtgcgtgccacagtgtccgcatcaggcgttgcagattgttgacggcaaagtggtgtggaacgctgtggtttgcgagcagtgtgatacctgccggaagaggtgtcagcaacatgCCACg                                                                                                                                                 <  1:63147‑M1/5‑1 (MQ=255)      ggTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCAATTCGTCCACGc                                                                                                                                                >  2:72734/1‑149 (MQ=255)                 gcAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCAATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTgc                                                                                                                                     >  1:659617/1‑149 (MQ=255)                      tGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGGCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCAt                                                                                                                                <  2:644053/149‑1 (MQ=255)                         ggATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACACACCGTTATCCCTTCGATAAACAGCACTGCTTTCCGGACATGGCTAAGCACTTCGTCCACACTCATGGCTTGCCCCATggc                                                                                                                             >  2:381946/1‑148 (MQ=255)                            tCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGt                                                                                                                          <  1:691386/149‑1 (MQ=255)                            tCGTTTGTGGTAGCAGTACACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGt                                                                                                                          <  2:17724/149‑1 (MQ=255)                             cGTGTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTg                                                                                                                         <  1:623022/149‑1 (MQ=255)                              gggggagtgcgtgccacagggtccgcatcaggcgtgggagattggggacggcaaagtggtgtggaacgctggggtttgcgagcagtgtgatccgtgcctgaagaggtgtgcgcaacatgCCACGCTCATGGATTGCGCCATGGGCGTgg                                                                                                                        >  2:67248‑M1/120‑149 (MQ=255)                               tttttGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTGGTCGGACATGGCTACGCACTTCGTCCACGCTCATGGCTTGCGCCATGGGCGTGGc                                                                                                                       >  1:403976/1‑149 (MQ=255)                                    gATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGtt                                                                                                                  >  1:346089/1‑149 (MQ=255)                                     aTAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGATCATGGAGTGCGCCATGGGCGTGGCctgctg                                                                                                                 >  2:551911/1‑149 (MQ=255)                                         cAGCAAACAGCACCACCACACATGGCAGCTGGGGCTTGGCTTCACGGTCACTCACCGTTATCCCTTCGATAAACAGGACTGCTTTGCGGACAGGGCTAAGCACTTCGTCCACGCTCATTGATTGCGCCATGGGCGTGGCATGTTGCGGa                                                                                                             <  2:395774/149‑1 (MQ=255)                                               aCAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACctc                                                                                                       >  1:273636/1‑149 (MQ=255)                                                   cgcCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTca                                                                                                   <  1:671232/149‑1 (MQ=255)                                                    gcCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTcag                                                                                                  >  1:534094/1‑149 (MQ=255)                                                     ccaccaCAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTcagg                                                                                                 >  1:312051/1‑149 (MQ=255)                                                      caccacAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCAACGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTcaggc                                                                                                <  2:451066/149‑1 (MQ=255)                                                        ccacAAACGGCAGCTGGGTCGTGGCTTCACGGCCACTCACAGTTAACACTTCGACAAACAGAACTGCTTTGAGAACATAGCTAGGAAATTCGTCCACGATCGTAGTTTGTGCATTGGGCTGGGCATGTTGCGGGCACATCTTcaggcag                                                                                              >  2:484121/1‑149 (MQ=255)                                                           caAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTa                                                                                           <  1:674826/149‑1 (MQ=255)                                                              aCGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATca                                                                                        <  1:38124/149‑1 (MQ=255)                                                                ggCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATcaca                                                                                      <  2:694989/149‑1 (MQ=255)                                                                     gattgttgacggcaaagtggtgtggaacgctgtggtttgcgagcagtgtgatacctgcctgaagaggtgtccgcaacatgCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTg                                                                                                                 >  1:635457‑M1/81‑117 (MQ=255)                                                                     gattgttgacggcaaagtggtgtggaacgctgtggtttgcgagcagtgtgatacctgcctgaagaggtgtccgcaacatgCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTg                                                                                                                 <  2:635457‑M1/37‑1 (MQ=255)                                                                     cTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCAGGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTCCt                                                                                 <  2:55424/149‑1 (MQ=255)                                                                           cctGGCTTCACCGCCACTCACCGGTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCaaa                                                                           <  2:572112/147‑1 (MQ=255)                                                                            gTGGCTTCAACGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAAc                                                                          <  1:60083/149‑1 (MQ=255)                                                                             tGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGTCAAGCACGTCGTCCACGCTCATAGATTGCGCCATGGGCGTGGCATGTTGCGCACAACTCTTCCGGCCAGTATCACACCGCCCACaacca                                                                         >  2:332320/1‑146 (MQ=255)                                                                              ggCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACca                                                                        <  1:212146/149‑1 (MQ=255)                                                                                cTTCACCGGCACTACCGGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACcaca                                                                      <  2:527391/149‑1 (MQ=255)                                                                                 ttCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAg                                                                     >  2:314751/1‑149 (MQ=255)                                                                                   cACCGGCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCg                                                                   <  2:632085/149‑1 (MQ=255)                                                                                    aCCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGt                                                                  >  1:662986/1‑149 (MQ=255)                                                                                           cTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGTGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCCGGTATCACACTGCTCGCAAACCACAGCGTTacacac                                                           >  1:5505/1‑149 (MQ=255)                                                                                           cTCACCGTTATCCCTGCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCacac                                                           <  1:314996/149‑1 (MQ=255)                                                                                                   tATCCCTACGAGAAACAGCCCTCCTTTGCTGACACGCCTAAGCCCTACACCACCCCTTATCGATTACGCCATGGGCATCGCACGTTGCGGACACCTCTTCAGGCAGGTATCACTCTGCTCCCACACCACAGCGTTCCACACCACTTTGc                                                   <  2:523911/149‑1 (MQ=255)                                                                                                    aTCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCGCTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATGACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGcc                                                  >  2:398837/1‑149 (MQ=255)                                                                                                        cTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACCCCACTTTGCCGTcc                                              >  1:680282/1‑148 (MQ=255)                                                                                                         ttCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTcaa                                             <  1:170159/149‑1 (MQ=255)                                                                                                         ttCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCAAACTGATCGCAAACCCCAGCGTTCCACACCCCCTTCCCgcaca                                             >  2:522220/1‑145 (MQ=255)                                                                                                             aTAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAAt                                         <  1:57794/149‑1 (MQ=255)                                                                                                             aTAAACAGCACCGCGTCGCGGACATGGCTCAGCACTTCGTCCACGCTCACGGATTGCGCCATGGGCGTGGCATGTTGCGGACCCCGCTTCAGGCAGGTATGACACTGCTCGCACACCACAGCGTTCCACACCACACCTCCGTCAACAAt                                         >  2:629988/1‑149 (MQ=255)                                                                                                                aaCAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTg                                      <  1:248721/149‑1 (MQ=255)                                                                                                                       cTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGcc                               >  1:622509/1‑149 (MQ=255)                                                                                                                        tGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCt                              <  1:170388/149‑1 (MQ=255)                                                                                                                        tGCTTTGCGGACATGGCTAAGCACTCCATCGGCGTGCGTGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCAAGCTGCTCGCAACCCACAGCGTTACACACCCCTTTCCCGTCAACAATCTGCCACGccc                              >  2:672200/1‑148 (MQ=255)                                                                                                                              gCGGACCTGGCAATGCATGTCGTCAACGCTCATCAATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGTGTTCCACATCACTTTGCCTTCAACAATCTGCAACGCCTGATGCg                        <  2:173574/149‑1 (MQ=255)                                                                                                                                ggACCTGGCTAAGCACTTCGTCCGCGCTCAGGGATTGCGCCATGGGAGTGGCATTTTGCGGACACCTCTTCATGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCGCTTTGCCGTCAACAATCTGCAACGCCTGATGCGGa                      <  2:459063/149‑1 (MQ=255)                                                                                                                                         tAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGgc             <  2:470278/149‑1 (MQ=255)                                                                                                                                            gCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTTAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGACAACAATCTGCAACGCCTGATGCGGCCACTGTGgcacg          >  1:557197/1‑149 (MQ=255)                                                                                                                                                    tCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTcccc  <  1:69386/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCC  >  NC_000913_3_hsa_tpiA/4615080‑4615375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.