New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 292646223 (0.970)4 (0.180) 4/270 2.7 15.1% coding (155/1365 nt) ygdH UPF0717 family protein
?NC_000913_3_hsa_tpiA = 2926466 23 (1.020)coding (159/1365 nt) ygdH UPF0717 family protein
Rejected: Frequency below/above cutoff threshold.

ATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCT  >  NC_000913_3_hsa_tpiA/2926320‑2926609
                                                                                                                                              |                                                                                                                                                   
atTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAAtt                                                                                                                                               >  1:167727‑M2/1‑147 (MQ=255)
     ctcgcTTGGCTCCATGGATATGTTGGCGCAGCTGGAGCTAAATATGTTTTAACGCACCGCCAGCAGCGCCCTCTATCAACTGTTTCGCAACGGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAAttgctgt                                                                                                                                          >  1:392308‑M2/3‑142 (MQ=255)
     cccGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAAttgctgt                                                                                                                                          >  2:329884‑M2/1‑142 (MQ=255)
      ccGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGtc                                                                                                                                         <  2:267148/149‑1 (MQ=255)
      ccGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGtc                                                                                                                                         >  1:249390/1‑149 (MQ=255)
           tGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGtt                                                                                                                                    <  2:124836/149‑1 (MQ=255)
                   tGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAttctttgctgttat                                                                                                                                       >  2:319247‑M1/1‑124 (MQ=255)
                   tGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAttctttgctgttat                                                                                                                                       <  1:319247‑M1/138‑15 (MQ=255)
                       tctgttgtCGCAGCTGGAACTGGATCTGCTGAAACGCCTCCCCAGCAGCGGCCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCg                                                                                                                        <  1:183238/147‑1 (MQ=255)
                        atGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGa                                                                                                                       <  1:415980/149‑1 (MQ=255)
                        atGGTGTCGCAGCTGGAAGTGGATCTGCCTAAACGCACCGCCAGCAGCGACCTTTATCAACTGTTTCGCAAGTGTTCACGTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGa                                                                                                                       <  1:415953/149‑1 (MQ=255)
                           ttgtCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGatat                                                                                                                    >  2:350284/1‑149 (MQ=255)
                                  aGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTc                                                                                                             <  2:249390/149‑1 (MQ=255)
                                                   cTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAttctttgctgttatcggtcaaactaccgga                                                                                                                       <  1:105642‑M1/122‑31 (MQ=255)
                                                   cTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAttctttgctgttatcggtcaaactaccgga                                                                                                                       >  2:105642‑M1/1‑92 (MQ=255)
                                                       aaCGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGATCTCGGGTGGTTTGTCCGTTAATAGCTAAGAATTGCTGTCTCGTTTTGTAAATTTCGATATTAACTTCTTGCGCTGTGATCGCGGCGTa                                                                                        >  1:194101/1‑149 (MQ=255)
                                                          gCACCGCCGGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAg                                                                                     <  1:371515/149‑1 (MQ=255)
                                                               gCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGa                                                                                >  1:281558/1‑149 (MQ=255)
                                                               gCCAGCAGCGACCTCTATCAACTGTTTCGCAAATGCTCACGTTCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGa                                                                                >  1:414512/1‑149 (MQ=255)
                                                                ccAGCAGCGACTTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTGGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGaa                                                                               <  1:210450/149‑1 (MQ=255)
                                                                              tATCAACTTTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATccccc                                                                 <  1:261948/149‑1 (MQ=255)
                                                                              tATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATccccc                                                                 >  2:373095/1‑149 (MQ=255)
                                                                                      gTTTCGCAACTGTTCACTTGCCGGACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGTACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAgg                                                         <  1:145948/149‑1 (MQ=255)
                                                                                                 ggttaCTTTCCGTACTGGACTCCGGTAGGTTTAGCGGTTACAGGACGCAATTGCTGTCTCGTTTTGAATATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAATCTGTAACTTATTAATCCCCCGGAAGAGGCTTGTGTCGAt                                              <  2:665/145‑1 (MQ=255)
                                                                                                   tCACTTGCCGTACTGAACTCCGGTAGTTTGACCGACAACAGCAAAGAATGGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATgg                                            <  1:221026/149‑1 (MQ=255)
                                                                                                     aCTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGttt                                                                                                                                   >  1:70111/1‑60 (MQ=255)
                                                                                                     aCTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGttt                                                                                                                                   <  2:70111/60‑1 (MQ=255)
                                                                                                                  aaCTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCgcgc                             >  1:193534/1‑149 (MQ=255)
                                                                                                                     tCCGGTAGTTTGACCGATAACAGCAAAGAAttgctgttatcggtcaaactaccggagttcagtacggcaagtgaacagttgcgaaacagttgatagaggtcgctgctggcggtgcgtttaag                                                     <  1:105642‑M2/122‑93 (MQ=255)
                                                                                                                     tCCGGTAGTTTGACCGATAACAGCAAAGAAttgctgttatcggtcaaactaccggagttcagtacggcaagtgaacagttgcgaaacagttgatagaggtcgctgctggcggtgcgtttaag                                                     >  2:105642‑M2/1‑30 (MQ=255)
                                                                                                                        ggTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCTCGCTTTGCa                       >  1:142040/1‑149 (MQ=255)
                                                                                                                                     aTAACAGCAAAGAAttgctgttatcggtcaaactaccggagttcagtacggcaagtgaacagttgcgaaacagttgatagaggtcgctgctggcggtgcgtttaagcatatccacttccagctgcgacaacatatcca                     >  2:319247‑M2/1‑14 (MQ=255)
                                                                                                                                     aTAACAGCAAAGAAttgctgttatcggtcaaactaccggagttcagtacggcaagtgaacagttgcgaaacagttgatagaggtcgctgctggcggtgcgtttaagcatatccacttccagctgcgacaacatatcca                     <  1:319247‑M2/138‑125 (MQ=255)
                                                                                                                                        aCAGCAAttctttgctgttatcggtcaaactaccggagttcagtacggcaagtgaaccgttgcgaaacagttgatagagggcgctgctggcggtgcgttaaaacatatttagctccagctgcgccaacatatccatggagccaagcgag       >  1:392308‑M1/1‑7 (MQ=255)
                                                                                                                                        aCAGCAAttctttgctgttatcggtcaaactaccggagttcagtacggcaagtgaacagttgcgaaacagttgatagaggtcgctgctggcggtgcgtttaagcatatccacttccagctgcgacaacatatccatggagccaagcggg       >  2:329884‑M1/1‑7 (MQ=255)
                                                                                                                                          aGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGGGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGt     >  1:129292/1‑149 (MQ=255)
                                                                                                                                             aattctttgctgttatcggtcaaactaccggagttcagtacggcaagtgaacagttgcgaaacagttgatagaggtcgctgctggcggtgcgtttaagcatatccacttccagctgcgacaacatatccatggagccaagcgggctaat  >  1:167727‑M1/1‑2 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCT  >  NC_000913_3_hsa_tpiA/2926320‑2926609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.