| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_hsa_tpiA | 3663442 = | 28 (1.180) | 6 (0.260) | 4/274 | 2.8 | 17.6% | coding (3029/3114 nt) | mdtF | anaerobic multidrug efflux transporter, ArcA‑regulated |
| ? | NC_000913_3_hsa_tpiA | 3663463 = | 29 (1.270) | coding (3050/3114 nt) | mdtF | anaerobic multidrug efflux transporter, ArcA‑regulated | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
GCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAA > NC_000913_3_hsa_tpiA/3663325‑3663609 | gCCCAATCCTGATGACCTCTCTGGCCTTTATTATCGGCGTGCTGCCGCTGGTTATCAGTGAGGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCCCGACCGTGCGGGCAAGTTACTTCggt > 2:83257/1‑147 (MQ=255) ccTCTTCGGTCTTTATTCGCGGCGGGCTGCCGCTGGTTAGCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTCTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGtt < 1:123736/149‑1 (MQ=255) ccTCTCTGGCCTTTATTCGCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGtt < 1:123663/149‑1 (MQ=255) tGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTg > 2:142996/1‑149 (MQ=255) cTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTACTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAAc < 1:149319/149‑1 (MQ=255) tttATTCTCGGCGTACTGCCGCTGGTTATCAGTCATGGTGCCGG‑TTCTGGCACGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTTCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTTGcaca > 2:407595/1‑149 (MQ=255) cTGGTTATCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTaaaaa > 2:151317/1‑149 (MQ=255) tATCAGTCATGCTGCCGG‑TTCTGGCGCGCAAAACGCGGGAGGTACCGGCTTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCTATATACTTCATTCCGCTCTTCTTCGTTGCAGAGGAAACACCCTTTGACCGCCTTaaaaaaccat > 2:329870/1‑145 (MQ=255) aggcATGGTTCCGGTTTCTGGC‑CCAAAAAAGCGGCATGTACCGGCTTTATGGTGGAGAGTTTTACCGCAACAGTGCTGTCAATTTACTTCTTCCCGGTCTTTTTCATTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACg < 2:235297/146‑1 (MQ=255) aGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCttt < 2:252334/126‑1 (MQ=255) aGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCttt > 1:252334/1‑126 (MQ=255) gTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACgt > 1:120996/1‑149 (MQ=255) ttttttaaagcgggcaaagagatgttccactacaacgaaaaagaccggaacgaagtaaaTTGCCGCAACAGTGCTGGCAATTTACtt < 2:389376‑M1/28‑1 (MQ=255) ttttttaaagcgggcaaagagatgttccactacaacgaaaaagaccggaacgaagtaaaTTGCCGCAACAGTGCTGGCAATTTACtt > 1:389376‑M1/60‑87 (MQ=255) cGG‑TTCTGGCGCGCAAAACGCGGTAGTGACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTCCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATgaga < 1:324108/149‑1 (MQ=255) gg‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAAGGagag > 1:96544/1‑149 (MQ=255) gcgcAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGaa > 1:216490/1‑96 (MQ=255) gcgcAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGaa < 2:216490/96‑1 (MQ=255) gcgcAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGa > 1:148762/1‑149 (MQ=255) aaaaCGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTGAAAAAAGCGTAACGTGTAAATGAGAGTCAGGCTGAACAt > 2:147992/1‑149 (MQ=255) aaCGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAAccttc < 2:148762/149‑5 (MQ=255) gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTActc > 1:181382/1‑149 (MQ=255) gCGTGATGGGCGGCATGTCTACCGCAACAGTGCTCGCAATTTACTTCGTTCCGGTGTTTTCCGTTGCAGTGGAACATCTCTTTGCCCGATTTAACAAAGCGTAATGTGGAAATTAGAGTAACATTGCACATGAAGGCGCAGCCTTActc > 2:331702/1‑149 (MQ=255) ctGGGCGGGTGGTTTGCCGCAACAGTCCTGGCACTGTCCTTCGTTCCGTCCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTcc < 1:201964/148‑1 (MQ=255) tGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCt > 1:48596/1‑149 (MQ=255) ggCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGc < 1:142996/149‑1 (MQ=255) ggCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGc < 1:169871/149‑1 (MQ=255) aagtaaattgccagcactgttgcggcaaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTaaaaaa > 1:389376‑M2/29‑87 (MQ=255) aagtaaattgccagcactgttgcggcaaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTaaaaaa < 2:389376‑M2/59‑1 (MQ=255) CGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTGAGCCTTACTCTTTCCTGCTAACCGTTcaccac < 1:346535‑M1/149‑1 (MQ=255) gCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACt > 1:132789/1‑149 (MQ=255) aaCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTTTTTGTTTTTGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTca > 2:71033/1‑149 (MQ=255) tgttgcggcaaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAgg > 1:6908‑M2/12‑95 (MQ=255) tgttgcggcaaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAgg > 2:323257‑M2/12‑95 (MQ=255) tgttgcggcaaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAgg < 1:323257‑M2/84‑1 (MQ=255) tgttgcggcaaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAgg < 2:6908‑M2/84‑1 (MQ=255) tGCTGGCAATTGTCTTCCTTCCGGGCTTTTTCGTTGCACTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGACGGGTCAGCCTTAATCTTTCCTCCTAACCATTCACCACTCaacaac < 1:331702/149‑1 (MQ=255) cTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCCTACTCTTTCCTGCTAACCATTCACCACTCAACAACCa < 1:281597/149‑1 (MQ=255) aaTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGTGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTaa < 1:318680‑M2/147‑1 (MQ=255) | GCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGG‑TTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAA > NC_000913_3_hsa_tpiA/3663325‑3663609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |