New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 2203961	21 (0.890)	4 (0.180)	4/272	2.7	16.6%	intergenic (+50/‑11)	yehI/yehK	DUF4132 domain‑containing protein/uncharacterized protein
	?	NC_000913_3_hsa_tpiA	= 2203988	20 (0.880)					coding (17/318 nt)	yehK	uncharacterized protein
Rejected: Frequency below/above cutoff threshold.

GTCACTTTTGCCGAACTTGATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAAC  >  NC_000913_3_hsa_tpiA/2203840‑2204131                                                                                                                                                     |                                                                                                                                                gTCACTTTTGCCGAACTTGATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAACGCGATGATCGTGCAAAAaga                                                                                                                                                 >  2:371482/1‑149 (MQ=255)      ttttGCCGAACTTGATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACCCGAGTTACCGGCGGGGAAAGCGATAATCGTGACCAAAGAgatgg                                                                                                                                            <  1:83953‑M2/149‑6 (MQ=255)      ttttGCCGAACTTGATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAgctgg                                                                                                                                            <  2:168057‑M2/149‑6 (MQ=255)                cgtGATGTGTTTTATTTGAGTGAGGCGTTAAGCGCACCTGACGTCATCTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTa                                                                                                                                  <  2:350154/147‑1 (MQ=255)                         ttttatttgaGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGa                                                                                                                         <  2:367091/149‑1 (MQ=255)                               ttgaGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTccc                                                                                                                   >  1:356776/1‑149 (MQ=255)                                    tgagGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTAc                                                                                                              >  1:236059/1‑149 (MQ=255)                                    tgagGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTAc                                                                                                              >  1:237986/1‑149 (MQ=255)                                           ttAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGa                                                                                                       >  1:329693/1‑149 (MQ=255)                                                           tcggtttCCTTTAAAACGCAGCGGGCAGTGATGCAACTGCCCGTTATCACCCCGACTTACCGGCGGGGAAAGCGATGAGCGTGCAAAAAGGGGTGGTGGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCata                                                                                       <  2:153120/145‑3 (MQ=255)                                                           tCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGa                                                                                       <  1:323504/149‑1 (MQ=255)                                                              ttttCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACtt                                                                                    <  2:329693/149‑1 (MQ=255)                                                                                 gggCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAAttccacc                                                                 >  2:209339/1‑143 (MQ=255)                                                                                           gCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGACTTATTTAc                                                       >  1:89236/1‑149 (MQ=255)                                                                                                tGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGACTTATTTACAGGtt                                                  >  1:207795/1‑149 (MQ=255)                                                                                                  cccGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGAACCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTcc                                                >  2:317645/1‑149 (MQ=255)                                                                                                    cGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAgctgg                                                                                                                                            <  1:263909‑M2/55‑6 (MQ=255)                                                                                                    cGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAgctgg                                                                                                                                            >  2:263909‑M2/1‑50 (MQ=255)                                                                                                    cGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGt                                              <  1:92315/149‑1 (MQ=255)                                                                                                    cGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCGGGTTGCTATTTACGATTATGAGGTCCCTGTACCGGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGt                                              <  1:371482/149‑1 (MQ=255)                                                                                                             cacGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAgctggtaagtcgtgttgataacgggcagttgcatcactgcccgctgtgttttaatggaaaatgacgtcaggtgcgcttaacgcctcactcaaatcaaacacatcaagt                                     <  2:230234‑M2/149‑109 (MQ=255)                                                                                                                gttgtACCATCTGGGAACGGGAGGATCGCGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCAGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGa                                  <  1:196182/145‑1 (MQ=255)                                                                                                                   ttACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAgctggtaagtcgtgttgataacgggcagttgcatcactgcccgctgtgttttaatggaaaat                                                                                   >  1:75531‑M2/1‑35 (MQ=255)                                                                                                                   ttACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAgctggtaagtcgtgttgataacgggcagttgcatcactgcccgctgtgttttaatggaaaat                                                                                   <  2:75531‑M2/97‑63 (MQ=255)                                                                                                                                 aGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTCTTTACAGGTTCCGTCTATCGACTGGAGCGATTCCGGCTACGTc                 >  1:21640/1‑149 (MQ=255)                                                                                                                                         tCGTGCAAAAAGAgctggtaagtcgtgttgataacgggcagttgcatcactgcccgctgtgttttaatggaaaatgacgtcaggtgcgcttaacgcctcactcaaatcaaacacatcaagttcggcaaaagtgacggatttattgtcac         >  1:230234‑M2/1‑13 (MQ=255)                                                                                                                                            tGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATCCTCTGAATTATTTACAGGTTCCGTCTATCTACTGGAGCGATTCCGGCTACGTCCAACATTccat      >  2:29450/1‑149 (MQ=255)                                                                                                                                                aaaaGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAAc  >  2:237911/1‑149 (MQ=255)                                                                                                                                                aaaaGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGCTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAAc  >  2:394152/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                GTCACTTTTGCCGAACTTGATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCATTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAGAGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAAC  >  NC_000913_3_hsa_tpiA/2203840‑2204131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.