New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	48 (1.420)	38/268	NT	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAT  >  NC_000913_3_hsa_tpiA/1300550‑1300844                                                                                                                                                     |                                                                                                                                                   ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                    <  1:22047‑M2/1‑1 (MQ=255) ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacaccataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagttctaaa                                                                                                                                                    >  2:86673‑M2/149‑149 (MQ=255)     caaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtggtaaataat                                                                                                                                                >  1:585006‑M2/145‑149 (MQ=255)              gaaagttatagggatttccttcactgctactccaaaccatcatgttattttcgccacatcataatcctgggcttcctgacgaataattgaactgatattattaattccactgcctttgatgtaggaaagtcctaaATAATAGTCAATTg                                                                                                                                       >  2:208409‑M2/136‑149 (MQ=255)                      taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcctaatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                               >  1:227518‑M2/128‑149 (MQ=255)                       aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                              <  2:643157‑M2/23‑1 (MQ=255)                        aggatttccttaactgcttcccctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                             <  1:525938‑M2/24‑1 (MQ=255)                         ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattcttaattccactgcctttggtatatgaaggtgctaaATAATAATCACTTGTTCAATTATtg                                                                                                                            >  2:269276‑M2/125‑149 (MQ=255)                         ggatttcctaaactgcttctcctcaccatcatgttattttcgcgacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATACTAATCAATTGTTAAATTATtg                                                                                                                            <  2:582947‑M2/25‑1 (MQ=255)                          gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataatttaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATAGTTAAATTATtgt                                                                                                                           >  2:8141‑M2/124‑149 (MQ=255)                           atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                          >  2:211385‑M2/123‑149 (MQ=255)                            tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctaggcctcctttaggataattgaaatgatattattaattccactgcctttggtagatgaaagtgcgaaATAATAATCAATTGTTAAATTATTGTGc                                                                                                                         >  1:385307‑M2/122‑149 (MQ=255)                              tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAt                                                                                                                       >  1:481408‑M2/120‑149 (MQ=255)                                cttaacttcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                     <  1:127564‑M2/32‑1 (MQ=255)                                 ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATCATCAATTGTTAAATTATTGTGCATTTc                                                                                                                    >  1:44043‑M2/117‑149 (MQ=255)                                       gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAttgtgcatttacccagt                                                                                                              <  2:82412‑M2/39‑8 (MQ=255)                                            tcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctagATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                         >  1:325811‑M2/106‑149 (MQ=255)                                            tcctcaccatcatgttattttagccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcgtttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                         <  1:421390‑M2/44‑1 (MQ=255)                                                  ccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                   <  2:120204‑M2/50‑1 (MQ=255)                                                   catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                  >  1:542005‑M2/99‑149 (MQ=255)                                                    atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAGATTATTTTNCATTTGAGTACTCGAACTGTAATCa                                                                                                 >  1:465162‑M2/98‑149 (MQ=255)                                                     tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAg                                                                                                >  1:2549‑M2/97‑149 (MQ=255)                                                     tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTAGAACTGTAATCAg                                                                                                >  2:30682‑M2/97‑149 (MQ=255)                                                        tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAACTACTGGAACTGTAATCAGaaa                                                                                             >  1:538069‑M2/94‑149 (MQ=255)                                                            aatttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                         <  2:48159‑M2/60‑1 (MQ=255)                                                             tttgcggcacatcataatccgtggcttgctgaagaataattgaaatgatattagtaattccactgcctttggtagaggaaagtactgaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                        <  2:403004‑M2/61‑1 (MQ=255)                                                             tttgcgccacatcataatccttggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTCCTACACAGTCCACTACTGGAACTGTAATCAGAAAAGATa                                                                                        <  2:471352‑M2/61‑1 (MQ=255)                                                                   ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                  >  1:640370‑M2/83‑149 (MQ=255)                                                                    cacatcataatcctgggattgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCATTACTGGAAATATAATAAGAAACGATAGACATGc                                                                                 >  2:375712‑M2/82‑149 (MQ=255)                                                                     acatcataatcctgggcttgctgacgcctaatacaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCAATACTGGAACTGTAATCAGATAAGTTATACTTGCt                                                                                >  2:305651‑M2/81‑149 (MQ=255)                                                                     acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCCGCTGGTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                                >  1:647590‑M2/81‑149 (MQ=255)                                                                      catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCtt                                                                               >  1:310733‑M2/80‑149 (MQ=255)                                                                        tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggagagtggtaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                             >  1:420295‑M2/78‑149 (MQ=255)                                                                        tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                             <  1:309363‑M2/72‑1 (MQ=255)                                                                                   ggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                                  <  2:421100‑M2/83‑1 (MQ=255)                                                                                       tgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                              <  2:421474‑M2/87‑1 (MQ=255)                                                                                                 taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaatggaa                                                    >  1:168508‑M2/53‑149 (MQ=255)                                                                                                  aattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                   <  1:248146‑M2/98‑1 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTCTTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATtgaaaaa                                                 >  2:111184‑M2/50‑147 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                 >  1:516320‑M2/50‑149 (MQ=255)                                                                                                    ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                 >  1:203822‑M2/50‑149 (MQ=255)                                                                                                    tggaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTGAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAGGCTTAGCCAATCTCTATTtgattggattgaagag                                                 >  1:523356‑M2/50‑146 (MQ=255)                                                                                                        aatgatattcacaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                             <  2:597127‑M2/104‑1 (MQ=255)                                                                                                         atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                            >  1:590812‑M2/45‑149 (MQ=255)                                                                                                              attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAACTCATTTAATGGAACGATGTTTGTTaa                                       >  1:311777‑M2/40‑149 (MQ=255)                                                                                                                          actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAGAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                           >  1:383172‑M2/28‑149 (MQ=255)                                                                                                                            tgcctttggtagaggaaagtgctaaATAATACTCAATTGTTCAATTATTGTGCATTTGACTACTCGAACTGTAATCAGAAAAGATAGACATGCTTAGCGAATCTGTATTTGATTGAATTGACAGATGTTTGTTAAGCCACGGCTGAAAg                         >  2:645231‑M2/26‑149 (MQ=255)                                                                                                                            tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATCGTGCATTTCACTAATGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTCAAGGCATGGATGAAAg                         >  2:645150‑M2/26‑149 (MQ=255)                                                                                                                                ttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                     <  2:450414‑M2/128‑1 (MQ=255)                                                                                                                                tttcgtagaggaaagtgataaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                     <  2:450476‑M2/128‑1 (MQ=255)                                                                                                                                  tagtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg                   <  2:26332‑M2/130‑1 (MQ=255)                                                                                                                                             agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGACTTGAAAGATGTTTGTTAAGGCATGGATGCCAGCTATAGATTATGATACg        >  2:506063‑M2/9‑149 (MQ=255)                                                                                                                                               tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCTTAAATGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt      >  1:456444‑M2/7‑149 (MQ=255)                                                                                                                                                  tAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTAATGGAACTGTAATCAGAAAAGATAGAACTGCTTAGATAATCTATATCAAATTAATTTGAATGATGTTTGTTAAGTCATGGATTCAAGCTGTAAATTCTGATACGGTGaa   >  2:567174/1‑149 (MQ=255)                                                                                                                                                   aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAt  >  2:556107‑M2/3‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAT  >  NC_000913_3_hsa_tpiA/1300550‑1300844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.