Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 482,230 G→A 100% P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA482,2300GA100.0% 130.4 / NA 47P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/22);  total (25/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_hsa_tpiA/482088‑482375
                                                                                                                                              |                                                                                                                                                 
gtaatcgttcttcGCGCGACCGGGGTCGATAAAGTCGTTCACATAGCTGCCTCCCCATGCAGCGCCCAGAGTGGTGTTAAGGTCGTTGATCGAAACACCCAGCGCCTGCACTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                             <  2:192908/142‑1 (MQ=255)
atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                             >  1:216826/1‑149 (MQ=255)
atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                             >  1:342637/1‑149 (MQ=255)
    aCTTTCTTCACACGGCCGCGGTCGATAAAGGCGTTCACATCGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTGTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                         <  2:260494/149‑1 (MQ=255)
      tttCTCCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                       <  2:390284/149‑1 (MQ=255)
       ttcttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTAATAGATAGACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAg                                                                                                                                      >  1:153059/1‑149 (MQ=255)
            cacaCGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCGGACCGt                                                                                                                                 >  1:26784/1‑149 (MQ=255)
                     gcgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTa                                                                                                                        <  1:551468/149‑1 (MQ=255)
                       ggTCGCTAAAGTCGGTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACg                                                                                                                      <  2:67817/149‑1 (MQ=255)
                           tacaaaGTCGGCCACATAGCCGCCCCCCCATGCAGCGCCCAGACCGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCCGTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                                  <  1:221059/146‑1 (MQ=255)
                           gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                                  >  1:505608/1‑149 (MQ=255)
                                    gTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACatat                                                                                                         >  2:57267/1‑149 (MQ=255)
                                               tGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTc                                                                                              <  1:466431/149‑1 (MQ=255)
                                                 ccgccCCACGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCCGCGCCTGCGCATTTTCCTTGTCTTTACCAAGCTTATACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                            <  2:401038/149‑1 (MQ=255)
                                                  cgccCTATGCAGCGCCTAGAGTTGTTTTAATTTTGTTGATAGAAATACCCAGCTGCTGCGTTTTTTCCTGGTCGATATCAATCTTAAACTTCAGGGTATCTTTCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgct                                                                                           <  2:96659/149‑1 (MQ=255)
                                                      ccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGTAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCtt                                                                                       <  1:35893/149‑1 (MQ=255)
                                                         tGCAGCGTCCAGAGTGGGGTTAATGTTGGTGATAGAAACACCCAGCGCCTGCGCTGTTTCCTGGTCGATATCAATCGTAAACTGCAGGGTATCTTCCAGACCGTTTGGGCGTACGCTGGTCAAGATATCAGGGTGCTTCGCTGCTTCTg                                                                                    <  2:372309/149‑1 (MQ=255)
                                                              cgcCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTATGTCCTCGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGATGCTTCGCTGCTTCGgcaagc                                                                               >  1:318613/1‑149 (MQ=255)
                                                                cccAGAGTGCTGTGAATGTCGTTGATAGAAACACACAGCGCATGCGCCTTTTCATGGTCGATATTAATCTTAAACTGAAAGGTAAACTACAGACCTTTTCGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaagcaa                                                                             <  2:165382/149‑1 (MQ=255)
                                                                 ccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCCGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCACCATCTCAGAGTGCTTCGCTCCCTCTgcaagcacc                                                                            >  2:553666/1‑147 (MQ=255)
                                                                 ccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCAGCGCTTTTTCCTGGTCGATGTCAAGCTTAAACTGCAGGGTATCTTCCAGACCGTATGGACGTACGATGCTCAACATCTCCGGGTGCTTCGCTGCTTCTGCACACAAc                                                                            >  2:365272/1‑149 (MQ=255)
                                                                  cAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACt                                                                           >  1:254036/1‑149 (MQ=255)
                                                                      gtggtgTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGtt                                                                       <  1:378082/149‑1 (MQ=255)
                                                                         gtgtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCTATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGGGCTTCGCTGCTTCTGCAAGCAACTGGTTAcg                                                                    >  2:273974/1‑149 (MQ=255)
                                                                           gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTAGATATCAATCTTAAACTGCAGGGTGTGTTCAAGGCCGTTGGGATGTTTGCTGGTCAACATATCTGGGTGCTTCTCTGCTTCTGCAAGGGACTGGTTAcggg                                                                  >  2:236799/1‑147 (MQ=255)
                                                                                tGTCGGGGAAAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                             <  2:655340/149‑1 (MQ=255)
                                                                                 gTCGTTGATAGAACCACCCAGCGCCTGCGCTTTTGCCGGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGCGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAg                                                            <  2:453057/149‑1 (MQ=255)
                                                                                    gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCACGCAACTGGTTACGCGCCTGAGTCa                                                         >  1:605044/1‑149 (MQ=255)
                                                                                      tGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTGTGCAAGCAACTGGTTACGCGCCTGAGTCAGt                                                       >  2:326779/1‑149 (MQ=255)
                                                                                         tAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTGGATCTCAATCTTAAACTGCAGGGGATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTGAGtctt                                                    >  1:520701/1‑149 (MQ=255)
                                                                                           gAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                  >  1:249341/1‑149 (MQ=255)
                                                                                                acCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc                                             <  1:97986/149‑1 (MQ=255)
                                                                                                  ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTgtctt                                           >  2:129766/1‑145 (MQ=255)
                                                                                                  ccAGCGCCTGCGCTTTTTCCTGATCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAGGTACGCTGGTCAACATATCAGGATGCTTCGCTGCTTGTGCAAGCAACCGGTTCCAATCCTGAGTACGTCTGTCGTGACCa                                           >  1:311660/1‑149 (MQ=255)
                                                                                                   cAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGGCGTACGCTGGTCAACATCACAGGGTGCTACGCCGCTTCTGCGAGCCACTGGTTACGCGCCTGAGGCAGCTTTTCTCGACCac                                          >  2:235125/1‑148 (MQ=255)
                                                                                                    aGCGTCGGCGTTTTTTCCTGGTCTATATGAATCTGAAACGGCAGGCTATCTTCCAGGCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                         <  2:448519/149‑1 (MQ=255)
                                                                                                          tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc                                   >  2:220981/1‑149 (MQ=255)
                                                                                                            cgcTTTCTCCTGGTCGATATCAATCTTAAACTGCAGGCTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATACCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt                                 <  2:463136/149‑1 (MQ=255)
                                                                                                             gatttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                                <  1:1044/147‑1 (MQ=255)
                                                                                                               tttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                              >  2:181608/1‑149 (MQ=255)
                                                                                                                ttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTc                             <  1:49561/149‑1 (MQ=255)
                                                                                                                    ccTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAGACTGTTCAACATATCAGGGTGCTTCGCTGCTTCTGCCAGCAACTGGTTACGAGCCTGAGTCAGTTTTTCTCGACCAAGGCCAGCCCGGTCAATc                         >  2:234488/1‑149 (MQ=255)
                                                                                                                         tCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc                    >  2:521103/1‑149 (MQ=255)
                                                                                                                                 aaTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTACTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTCCCCCAGGCCAACCTGGGCACACGGCtcacagccaa            >  2:275263/1‑149 (MQ=255)
                                                                                                                                       aaaCGGCAGAGTATCCTCCAGACCGTTTGGACGCACGCTGGTCAACATATCAGGGTGCTTCGCTGCTGCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg      <  1:162831/149‑1 (MQ=255)
                                                                                                                                        aaCTGCAGGGTACCTTCCAGACCGTTTGCACGTACACTGGACAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt     <  1:344127/149‑1 (MQ=255)
                                                                                                                                           tGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTAAAAGCTGGTAGc  >  1:614622/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_hsa_tpiA/482088‑482375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑