| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_hsa_tpiA | 1,196,232 | C→T | 20.2% | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 1,196,232 | 0 | C | T | 20.2% | 37.5 / 2.3 | 25 | T370T (ACC→ACT) | icd | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
| Reads supporting (aligned to +/- strand): ref base C (12/7); new base T (2/3); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAA > NC_000913_3_hsa_tpiA/1196084‑1196373 | gtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCACTGTTTGAAGCCACACACGGTACTGCGCCGAAATATGCCGGTGAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGCGATGATGCTGCGCCACGTGGGTTGGAcc > 1:142197/1‑149 (MQ=255) tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCAGGCTCTGTTAGTCTCTCCGCTGAGATGATGCTGCGCCATCTGGGTTGTACTGAAGCGGCTGagc > 2:235949/1‑147 (MQ=255) tGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGTTGGCTTAATTGTTAAAGGTATGGa > 2:357126/1‑149 (MQ=255) ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 2:433711/1‑149 (MQ=255) cTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGTaatacac > 1:225050/1‑144 (MQ=255) cTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcgc > 2:145456/1‑149 (MQ=255) tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGATCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGTGCAAt > 2:394536/1‑149 (MQ=255) ttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTGTTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc > 1:212080/1‑149 (MQ=25) aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGATAAAGTAAAGCCGGGCTCTATGATGCTCTCCGCGGAGATGATGCGGCGCCACATGGGTGGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc < 1:630540/149‑1 (MQ=37) aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATAGGTTGGACCGAAGCGGCTGACTTACTTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:566998/1‑149 (MQ=255) gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcgc < 1:373088/149‑1 (MQ=255) acccacGGAACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAAGTTGCGCCACATGGGCTCGACCGAAGAGGCTGACTGAATTGTTAGAGCTGTGGACGGAGCAATCAACGCGaa > 2:557070/1‑149 (MQ=255) tACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa < 2:593252/149‑1 (MQ=255) cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:163134/149‑1 (MQ=255) gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATTATGCTGAGCCACATGAGTTGTACCGAACCGGCTGACTTAATTGTTAAAGTTATGGAAGGCGGAATCAACACGAAAACCGCACCATATg > 2:579194/1‑149 (MQ=255) gAAATATGCCGGTCAGAACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGGTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAGTGAGCAATCAACGCGAATACCGTAGCCTATGACt > 2:59013/1‑149 (MQ=255) gAAATATGCCGATAAGGAAAAATTAAATTGTGGCTCTATTATTCTATGCGCTGAGATGATGCTGCGCAAAATGGGTTGGAATGAAGTGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACTTATGACt < 2:226602/149‑1 (MQ=18) tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTTGCCGCTTAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac < 2:199509/149‑3 (MQ=14) aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGACCGTCTgatggat > 1:54991/1‑149 (MQ=255) agtcTGGCTCTACCATTCTCCACACTGAGATGGTGCTGCGCCACATGGCTCGGACTGAAGCGCCTGACTTAATTGTCAAAGGTACGGAAAGCGCAATGAACGCGAACACCGTAACCTACGACTTCGAGCGTCTGATGGATGGCGCTaaa < 2:84957/146‑1 (MQ=255) gagaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGATAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa < 1:645928/149‑1 (MQ=255) gatggctcgccgcATGGGGTGGACCGAAGCGGTTGACTTAGTTGTTGAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa < 2:460445/142‑1 (MQ=18) ctgcGCGACATGGGTTGGACGGAAGCGGCTGACTTAATTGTGAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATTACTTCGAGTGTCTGATGGATAATGTTTATTTGCTGAAATGTTCAGAGTTTGGTGACGCGatcat < 2:112085/149‑1 (MQ=17) cacaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATAGaaaa > 1:643042/1‑149 (MQ=255) acaTGGGCTGGACCGAACCGAATGACTTAATTGTTCGAGGCCTGGAAGGCTCAATCAACGCGAAAACCGTGACCTATGACTGCAAGTGTCCGATGGATGGCGCTAAACTGCTGACATGTTCTGAGTTTGGTGACGCCGTCATCGCAAAc > 2:71567/1‑149 (MQ=255) gTTCGACCGAAGTGGCTGACTTAACTGTTAAAGGTATGTAAGGCGCTATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGGGCGAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa < 2:340882/149‑1 (MQ=17) | GTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAA > NC_000913_3_hsa_tpiA/1196084‑1196373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |