BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	389,925	(T)_6→7	intergenic (‑198/‑326)	hemB ← / → yaiT	5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	389,919	1	.	T	100.0%	360.8 / NA	98	intergenic (‑192/‑332)	hemB/yaiT	5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (50/48); total (50/48)

Only 100 of 106 total aligned reads displayed.

AAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑TTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAA  >  NC_000913_3_hsa_tpiA/389771‑390059

aaGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTtatta‑t                                                                                                                                             >  1:1165968/1‑150 (MQ=255)

     gggTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGAAAAGAAGCGTATGGAAGGGAGGCCAG‑GACATCTTGTTAATAGAACCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttt                                                                                                                                        <  3:321634/150‑1 (MQ=255)

      ggTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑ttttttt                                                                                                                                       <  1:267257/150‑2 (MQ=255)

       gTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttg                                                                                                                                      >  1:601694/1‑148 (MQ=255)

       gTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttg                                                                                                                                      <  2:205473/150‑3 (MQ=255)

       gTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttg                                                                                                                                      <  4:130894/150‑3 (MQ=255)

         aGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGGATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttgtg                                                                                                                                    >  2:1062552/1‑146 (MQ=255)

         aGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttgga                                                                                                                                    >  1:1233402/1‑146 (MQ=255)

            gATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGtt                                                                                                                                  >  2:178872/1‑150 (MQ=255)

              tGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTg                                                                                                                                <  1:621437/150‑1 (MQ=255)

                ttttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGt                                                                                                                              <  3:186888/150‑1 (MQ=255)

                  ttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTg                                                                                                                            >  2:542859/1‑150 (MQ=255)

                       aaaGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctg                                                                                                                       >  2:1119275/1‑150 (MQ=255)

                        aaGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctgc                                                                                                                      <  2:897115/150‑1 (MQ=255)

                         aGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctgct                                                                                                                     <  1:1148071/150‑1 (MQ=255)

                           ttGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCtat                                                                                                                   <  4:339733/150‑1 (MQ=255)

                              tCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaaa                                                                                                                >  1:719445/1‑150 (MQ=255)

                              tCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaaa                                                                                                                <  4:608219/150‑1 (MQ=255)

                                gcgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAAtt                                                                                                              >  3:257630/1‑150 (MQ=255)

                                gcgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAAtt                                                                                                              <  1:270020/150‑1 (MQ=255)

                                 cgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                             <  1:157047/150‑1 (MQ=255)

                                  gATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGa                                                                                                            <  3:739410/150‑1 (MQ=255)

                                     gTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaata                                                                                                         >  3:568499/1‑150 (MQ=255)

                                        gACAAGGAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAAtt                                                                                                      >  1:452183/1‑150 (MQ=255)

                                          cAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTaa                                                                                                    <  3:91888/150‑1 (MQ=255)

                                           aagaagAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTaaa                                                                                                   >  2:316943/1‑150 (MQ=255)

                                            agaagaGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTaaaa                                                                                                  >  2:61029/1‑150 (MQ=255)

                                            agaagaGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTaaaa                                                                                                  <  1:497882/150‑1 (MQ=255)

                                                gagaATGGAAGAGAGGCCAG‑GACATGTTTTTAATTGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                              <  2:179447/150‑1 (MQ=255)

                                                gagaATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                              <  3:463854/150‑1 (MQ=255)

                                                gagaATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                              >  1:677447/1‑150 (MQ=255)

                                                 agaATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttg                                                                                             <  1:709299/150‑1 (MQ=255)

                                                 agaATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttg                                                                                             >  1:963254/1‑150 (MQ=255)

                                                   aaTGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                           >  2:1005941/1‑150 (MQ=255)

                                                   aaTGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                           <  3:200139/150‑1 (MQ=255)

                                                   aaTGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                           <  1:476225/150‑1 (MQ=255)

                                                   aaTGGAAGAGA‑GCGAGTGACTGCTGTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGTTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                           <  1:998211/150‑1 (MQ=255)

                                                     ttgAAGAGAAGTAAG‑GACATCTTTTTAATAGAAACAGTAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTTGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATCATTAAAACTTTGTTGc                                                                                         <  2:643664/148‑1 (MQ=255)

                                                      ggAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCa                                                                                        >  3:722458/1‑150 (MQ=255)

                                                       gAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCat                                                                                       >  2:155106/1‑150 (MQ=255)

                                                       gAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCaa                                                                                       >  1:643664/1‑149 (MQ=255)

                                                       gAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTAGGTTATTATTTTTTTGAAGTTTGGTTGCGTTGATATATATTGAATATTTAAAACTTTGTTGCat                                                                                       >  1:1044339/1‑150 (MQ=255)

                                                       gAAGAGAGGCCAG‑GACAGCTTTTGAATAGAAACTGCAATAATTTTATATTCACGGTAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTTAATAATTAAAACTTTGTTGCat                                                                                       >  2:429403/1‑150 (MQ=255)

                                                        aaGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCata                                                                                      >  1:946888/1‑150 (MQ=255)

                                                          gagagGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATAtt                                                                                    >  3:52846/1‑150 (MQ=255)

                                                            gagGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTg                                                                                                                                >  2:366632/1‑104 (MQ=255)

                                                            gagGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTg                                                                                                                                <  1:366632/104‑1 (MQ=255)

                                                             agGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCAt                                                                                 <  2:1165968/150‑1 (MQ=255)

                                                               gCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCatat                                                                                     >  4:371691/1‑144 (MQ=255)

                                                               gCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCatat                                                                                     <  3:371691/144‑1 (MQ=255)

                                                                     gACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTTTTTTTTTTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGatat                                                                          >  2:734057/1‑150 (MQ=255)

                                                                     gACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGa                                                                                                            <  1:823367/116‑1 (MQ=255)

                                                                     gACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATACTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGa                                                                                                            >  2:823367/1‑116 (MQ=255)

                                                                         tCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTAt                                                                      >  1:1148358/1‑150 (MQ=255)

                                                                          cTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATc                                                                     >  1:1168813/1‑150 (MQ=255)

                                                                          cTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATc                                                                     >  3:273952/1‑150 (MQ=255)

                                                                           tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCt                                                                    >  1:1096466/1‑150 (MQ=255)

                                                                           tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCt                                                                    <  4:223896/150‑1 (MQ=255)

                                                                           tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCt                                                                    <  2:1000074/150‑1 (MQ=255)

                                                                              ttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgt                                                                 <  2:241526/150‑1 (MQ=255)

                                                                               tAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtt                                                                <  1:61029/150‑1 (MQ=255)

                                                                               tAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtt                                                                >  4:138405/1‑150 (MQ=255)

                                                                                  tAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgt                                                             <  3:512798/150‑1 (MQ=255)

                                                                                  tAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgt                                                             <  3:774302/150‑1 (MQ=255)

                                                                                    gAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgt                                                           <  2:1084543/150‑1 (MQ=255)

                                                                                      aaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgttt                                                         <  1:178872/150‑1 (MQ=255)

                                                                                      aaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgttagtgttt                                                         <  4:588862/150‑1 (MQ=255)

                                                                                        cAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtttgt                                                       <  2:276275/150‑1 (MQ=255)

                                                                                         aGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGTAGTTTGGTTTCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGtttgtgtttgtg                                                      >  2:705207/1‑150 (MQ=255)

                                                                                            aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGata                                                   <  3:557594/150‑1 (MQ=255)

                                                                                             ataatTTTATATTCACCGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGAATATTTACCTGGTTTGTGTTTGTGatat                                                  <  2:1185772/150‑1 (MQ=255)

                                                                                              taatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatt                                                 >  1:510870/1‑150 (MQ=255)

                                                                                                 ttttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttat                                              <  4:568499/150‑1 (MQ=255)

                                                                                                  tttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttatt                                             >  1:347418/1‑150 (MQ=255)

                                                                                                    tataTTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTtattat                                           <  1:1039831/150‑1 (MQ=255)

                                                                                                       atTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCg                                        >  3:181250/1‑150 (MQ=255)

                                                                                                         tCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTTTTTTTTTTTGTGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCTTAGGATATTTATTTGTTTTGTGTTTGTGATATTTATTATTCGtt                                      >  4:274140/1‑149 (MQ=255)

                                                                                                           aCTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATg                                    <  1:1094210/150‑1 (MQ=255)

                                                                                                             tGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                           <  4:59405/93‑1 (MQ=255)

                                                                                                             tGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                           >  3:59405/1‑93 (MQ=255)

                                                                                                              gAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCtt                                 >  1:624163/1‑150 (MQ=255)

                                                                                                               aaaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTc                                >  1:335935/1‑150 (MQ=255)

                                                                                                               aaaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTc                                >  1:422918/1‑150 (MQ=255)

                                                                                                               aaaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTc                                >  3:337522/1‑150 (MQ=255)

                                                                                                                  atatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAGTAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGTTATTTATTATGCGTATGCTTCaaa                             >  1:1049881/1‑150 (MQ=255)

                                                                                                                     tttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTTAGTTTTGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAAc                          >  2:312327/1‑150 (MQ=255)

                                                                                                                        ttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaa                       >  2:101892/1‑150 (MQ=255)

                                                                                                                        ttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaa                       <  1:1005941/150‑1 (MQ=255)

                                                                                                                               ttatttatAGCTGTTGGTTATTATTTTTTTGGTGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCt                >  4:239127/1‑150 (MQ=255)

                                                                                                                               ttatttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCt                >  3:635956/1‑150 (MQ=255)

                                                                                                                               ttatttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCt                >  1:1236961/1‑150 (MQ=255)

                                                                                                                                tatttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTg               <  2:388103/150‑1 (MQ=255)

                                                                                                                                   ttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCaa            <  3:601184/150‑1 (MQ=255)

                                                                                                                                     ataGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGTTATTTTTTATTCGTATTCTTCTTAAACAAAATTAAATTCAACa          >  2:468203/1‑150 (MQ=255)

                                                                                                                                          tGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTg     >  2:612502/1‑150 (MQ=255)

                                                                                                                                           gTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGa    <  2:707736/150‑1 (MQ=255)

                                                                                                                                             tGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGaaa  <  1:542859/150‑1 (MQ=255)

                                                                                                                                             tGGTTATTATTTTTTTGGAATTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATAATTCAAAAACAAAATTATCTGCAACATGTTGaaa  <  4:275657/150‑1 (MQ=255)

                                                                                                                                               gTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCa                               >  2:256032/1‑119 (MQ=255)

                                                                                                                                               gTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCa                               <  1:256032/119‑1 (MQ=255)

AAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAG‑GACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑TTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAA  >  NC_000913_3_hsa_tpiA/389771‑390059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`