Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,187,572	G→T	M747I (ATG→ATT)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,187,572	0	G	T	100.0%	301.3 / NA	85	M747I (ATG→ATT)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/44);  total (41/44)

GGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACC  >  NC_000913_3_hsa_tpiA/4187425‑4187717                                                                                                                                                    |                                                                                                                                                  ggATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGc                                                                                                                                                 <  1:314201/150‑1 (MQ=255)  gATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCg                                                                                                                                                <  4:326420/150‑1 (MQ=255)  gATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCg                                                                                                                                                <  1:253395/150‑1 (MQ=255)  gATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCg                                                                                                                                                <  4:211306/150‑1 (MQ=255)  gATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCg                                                                                                                                                <  4:211209/150‑1 (MQ=255)   aTAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGa                                                                                                                                               >  4:474611/1‑150 (MQ=255)   aTAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGAGTGCGa                                                                                                                                               >  4:666012/1‑150 (MQ=255)      aCCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGc                                                                                                                                            <  3:297358/150‑1 (MQ=255)       ccTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGcc                                                                                                                                           <  1:1131961/150‑1 (MQ=255)           cAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGAt                                                                                                                                       <  2:828858/150‑1 (MQ=255)               cTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCt                                                                                                                                   <  1:803136/150‑1 (MQ=255)                tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTc                                                                                                                                  <  1:411642/150‑1 (MQ=255)                tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTc                                                                                                                                  <  2:351990/150‑1 (MQ=255)                 gAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTcc                                                                                                                                 <  4:550896/150‑1 (MQ=255)                  aaaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCca                                                                                                                                <  2:1176614/150‑1 (MQ=255)                      cGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCcatcat                                                                                                                            <  2:93540/150‑1 (MQ=255)                        tGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCg                                                                                                                          >  3:744523/1‑150 (MQ=255)                              aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGc                                                                                                                    <  1:270796/150‑1 (MQ=255)                                cGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                                                                                                                  >  1:716187/1‑150 (MQ=255)                                     cGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAc                                                                                                             >  3:415916/1‑150 (MQ=255)                                      ggTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAcc                                                                                                            <  3:440109/150‑1 (MQ=255)                                       gTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACAGGATGGCCGACGCCGTTGCGCGTGTTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACcg                                                                                                           <  1:305436/150‑1 (MQ=255)                                          aGGAAGAGAAGGAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGa                                                                                                        >  4:653474/1‑150 (MQ=255)                                          aGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGa                                                                                                        >  1:511248/1‑150 (MQ=255)                                           ggAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAAc                                                                                                                      <  3:570604/135‑1 (MQ=255)                                           ggAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAAc                                                                                                                      >  4:570604/1‑135 (MQ=255)                                            gAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCGGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAGCGCCAATCACCGCGAAc                                                                                                      >  2:907407/1‑150 (MQ=255)                                            gAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAAc                                                                                                      >  2:475663/1‑150 (MQ=255)                                                agaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTcc                                                                                                  <  3:474611/150‑1 (MQ=255)                                                     cAGGTTTCCTTCAACAGCATCTACATTATTGCCGTCTCCGATGCGCGTTCTACACCTGCACATAGTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGaa                                                                                             <  3:457188/150‑1 (MQ=255)                                                        gTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGt                                                                                          >  2:999789/1‑150 (MQ=255)                                                              ttCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAACCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc                                                                                    >  2:1186623/1‑150 (MQ=255)                                                                   cAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACt                                                                               >  3:104490/1‑150 (MQ=255)                                                                    aGCATCTCCATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGGCTGATTGCGAAGCCGGATGGCTCCATCAACCAAACGCCAGGCACCGCGAACTTCCGTGAAGGTCTGAACGTACTc                                                                              <  1:905751/150‑1 (MQ=255)                                                                    aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTc                                                                              <  2:1138099/150‑1 (MQ=255)                                                                    aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTc                                                                              <  2:716187/150‑1 (MQ=255)                                                                     gCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                                                                                                    >  1:1208785/1‑127 (MQ=255)                                                                     gCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                                                                                                    <  2:1208785/127‑1 (MQ=255)                                                                       ntCTACATGATGGCCGACTCCGGTGCGCGTNGNTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAg                                                                           <  2:957789/149‑1 (MQ=255)                                                                       aTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACTGCGAACTTCCGTGAAGGTCTGAACGTACTCCAg                                                                           <  2:211793/150‑1 (MQ=255)                                                                       aTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAg                                                                           >  4:388684/1‑150 (MQ=255)                                                                           aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACt                                                                       >  1:827576/1‑150 (MQ=255)                                                                               gatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAt                                                                   >  3:84192/1‑150 (MQ=255)                                                                               gatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAt                                                                   >  1:789089/1‑150 (MQ=255)                                                                                 tgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAtct                                                                 >  1:514976/1‑150 (MQ=255)                                                                                     cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGGTGGCTCCATCATCGAAACGCCAATCACNGNGAACTTCCGGGAAGGTCTGAACGTACTCCAGTACTTCATCTccac                                                             <  3:120707/150‑1 (MQ=255)                                                                                     cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccac                                                             >  4:756877/1‑150 (MQ=255)                                                                                     cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccac                                                             >  1:806217/1‑150 (MQ=255)                                                                                             gTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTg                                                     <  4:222630/150‑1 (MQ=255)                                                                                                 gcgTGGTTCTGCGGCACGGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                                                                                                    <  1:977430/99‑1 (MQ=255)                                                                                                 gcgTGGTTCTGCGGCACGGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                                                                                                    >  2:977430/1‑99 (MQ=255)                                                                                                 gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc                                                                                    >  1:11096/1‑115 (MQ=255)                                                                                                 gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc                                                                                    <  2:11096/115‑1 (MQ=255)                                                                                                 gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCg                                                 <  1:210933/150‑1 (MQ=255)                                                                                                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGt                                                >  3:476085/1‑150 (MQ=255)                                                                                                    tGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTaa                                              <  3:653474/150‑1 (MQ=255)                                                                                                       ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                           >  1:881293/1‑150 (MQ=255)                                                                                                       ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                           >  2:680646/1‑150 (MQ=255)                                                                                                       ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                           >  2:168397/1‑150 (MQ=255)                                                                                                        tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGaa                                                                                     <  2:583377/107‑1 (MQ=255)                                                                                                        tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGaa                                                                                     >  1:583377/1‑107 (MQ=255)                                                                                                           gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTc                                                                              >  3:112116/1‑111 (MQ=255)                                                                                                           gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTc                                                                              <  4:112116/111‑1 (MQ=255)                                                                                                            cGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTcca                                                              >  2:571912/1‑126 (MQ=255)                                                                                                            cGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTcca                                                              <  1:571912/126‑1 (MQ=255)                                                                                                            cGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTgg                                      >  1:781764/1‑150 (MQ=255)                                                                                                              gCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCg                                    <  2:514976/150‑1 (MQ=255)                                                                                                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTcca                                                              >  2:91420/1‑120 (MQ=255)                                                                                                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTcca                                                              <  1:91420/120‑1 (MQ=255)                                                                                                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATa                                <  1:475663/150‑1 (MQ=255)                                                                                                                   gATTCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                               >  1:509506/1‑150 (MQ=255)                                                                                                                     ttCGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCg                             <  4:415916/150‑1 (MQ=255)                                                                                                                       cGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAGTCACCGCGAACGTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCa                           <  1:1045697/150‑1 (MQ=255)                                                                                                                       cGTCAGCTTGCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCa                           <  2:511248/150‑1 (MQ=255)                                                                                                                                gCTGGTATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACt                  >  1:217358/1‑150 (MQ=255)                                                                                                                                     tATGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGaa             <  2:827576/150‑1 (MQ=255)                                                                                                                                      aTGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGGACTGAAAACTGCGAAc            >  1:998214/1‑150 (MQ=255)                                                                                                                                       tGCGTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACt           >  3:541499/1‑150 (MQ=255)                                                                                                                                          gTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                                                                                                                  >  2:1222784/1‑44 (MQ=255)                                                                                                                                          gTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                                                                                                                  <  1:1222784/44‑1 (MQ=255)                                                                                                                                          gTGGTCTGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCg        >  4:411509/1‑150 (MQ=255)                                                                                                                                                tGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                >  1:78421/1‑136 (MQ=255)                                                                                                                                                tGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                <  2:78421/136‑1 (MQ=255)                                                                                                                                                tGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAcc  >  1:1073770/1‑150 (MQ=255)                                                                                                                                                tGATTGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGCGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAcc  >  3:629893/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACC  >  NC_000913_3_hsa_tpiA/4187425‑4187717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑