Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 1,544,021:1 +A 75.9% coding (40/1389 nt) narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,544,0211.A75.9% 67.6 / 19.8 29coding (40/1389 nt)narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base . (2/5);  new base A (8/14);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGT  >  NC_000913_3_hsa_tpiA/1543885‑1544154
                                                                                                                                          |                                                                                                                                      
ttAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAAc                                                                                                                            >  1:38861/1‑149 (MQ=255)
           tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTtc                                                                                                                 >  1:490901/1‑149 (MQ=255)
                     aGCATCAAGACACAGAAGTCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCa                                                                                                       <  2:90454/149‑1 (MQ=255)
                          ccAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTCTTTTTCTCATTTTGCAGTGcc                                                                                                  <  1:318057/149‑1 (MQ=255)
                                     aGGCAAGAAGTAGACAATTGACTGATATCCAGAGATTTCTTGGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                       <  1:357258/149‑1 (MQ=255)
                                       gtaagaaaTAGATAACTGCCTAATATCAAGAAATACCGTCGAGCAATATGTTTTCATTTATTTACCCAGAAGGCCGGATTTTCTTGTTAAAAAAAACAAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                     <  2:21835/147‑1 (MQ=255)
                                       gCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTAGCGCCATCGGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                     <  1:509311/149‑1 (MQ=255)
                                             aggaaaCAACTGACTGATATCCAGAGATTTCTTCGAGCAATTTGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCCGTGCCATATCGTTCCTCACATGca                                                                               <  1:532060/144‑1 (MQ=255)
                                               tAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                             >  1:584860/1‑149 (MQ=255)
                                                 gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaccca                                                                           >  1:310921/1‑149 (MQ=255)
                                                 gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCATTTATTTTCCAAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTGTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcagaca                                                                           >  2:8864/1‑149 (MQ=255)
                                                 gACAACCGACTGATAGCCAGAGAGCTCATCGAGCAATATGTTATCATATATATACACAGAAGGCCGGATTTTCTAGTAAACAAACAAAAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacaca                                                                           <  2:76481/149‑1 (MQ=255)
                                                    aaCTGACTGATATCCAGAGATTGCTTCGAGCAATATGTTTTTCTTGATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                        <  2:9963/149‑1 (MQ=255)
                                                       tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACAACTATTTTTCTCATTTTGCAGTCCAATATTCTTCCTCACCTGCCCACATTAGtc                                                                     >  2:189831/1‑148 (MQ=255)
                                                        ggctgaTATCCAGAAATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCATTGCAATATTGTTGCTCACATGCACACATTGGTaa                                                                    <  2:378380/147‑1 (MQ=255)
                                                         actgaTATCCAGTGGTTTCTTCTAGCAATAGGTTTTGCTTTGTTGTCCCAGTAGTCCTGAGTTTCTGGTTTCCAGTCGCGATAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAAt                                                                   <  2:496860/149‑1 (MQ=255)
                                                             atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaa                                                               <  1:188434/149‑1 (MQ=255)
                                                                  cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTACCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                          >  1:373979/1‑149 (MQ=255)
                                                                              tgtgCAGTATTTTTTCCTTTATTTTCCCAGAAGTCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACGATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAg                                              <  1:46889/146‑1 (MQ=255)
                                                                                 tcAATATGTTTTCTTTTTTCTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCCAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCTATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTa                                           <  1:14181/148‑1 (MQ=255)
                                                                                            accTTTATGTGACCGGCAGGTCCGATCTTCTGGTTTCCAGTCGAGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAg                                <  2:182134/148‑1 (MQ=255)
                                                                                               tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                             >  1:430423/1‑149 (MQ=255)
                                                                                                         cAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGTCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                   <  1:398012/149‑1 (MQ=255)
                                                                                                                ccGGATTATCTGGTTTCCAGGCGCAAAAAAAGATAACGACTAATGTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGGAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGa            <  2:289443/149‑1 (MQ=255)
                                                                                                                 cGGATTTTCTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt           <  1:282908/149‑1 (MQ=255)
                                                                                                                         ttGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg   <  1:391696/148‑1 (MQ=255)
                                                                                                                         cTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCTTTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg   <  1:437740/149‑1 (MQ=255)
                                                                                                                         cTGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACAACAGGTAAGGCGCAATAGCCAGTTATTAGACTTAAGGATGACTTggg   >  2:569492/1‑149 (MQ=255)
                                                                                                                          tGGTTTCCAGTCGCGAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGt  >  1:504724/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGT  >  NC_000913_3_hsa_tpiA/1543885‑1544154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: