Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913_3_hsa_tpiA	1,726,433	Δ11 bp	38.3%	coding (411‑421/600 nt)	nemR →	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1726432	14 (0.430)	9 (0.280)	9/276	NT	38.3%	coding (410/600 nt)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
	?	NC_000913_3_hsa_tpiA	1726444 =	15 (0.460)					coding (422/600 nt)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

TTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAC  >  NC_000913_3_hsa_tpiA/1726289‑1726577                                                                                                                                                |                                                                                                                                                  ttaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCtctctc                                                                                                                                              >  1:40663/1‑149 (MQ=255)     cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGgc                                                                                                                                          >  1:455627/1‑149 (MQ=255)           acacTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaattgccgtg                                                                                                                                    >  1:59653‑M1/1‑134 (MQ=255)             acTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaaa                                                                                                                                  >  1:588151/1‑149 (MQ=255)                 aaCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGCTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATgg                                                                                                                              >  1:553578/1‑149 (MQ=255)                 aaCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaacc                                                                                                                              >  1:381689‑M1/1‑128 (MQ=255)                             cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgttaaatct                                                                                                                  >  1:139990‑M1/1‑116 (MQ=255)                                  tGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGTAAAATGGCCGTGAGAACCATTGtt                                                                                                             >  2:213584/1‑149 (MQ=255)                                     aaCCACCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTAAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTaa                                                                                                          <  1:264662/149‑1 (MQ=255)                                        cATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGAATAAAGGTGCTAGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaa                                                                                                       >  1:548096‑M1/1‑105 (MQ=255)                                                gTTGCCTGACAGTAAAACTCTCTGACGAAGTGTGCGATCTGTCAGAAGATATGCGCAGAGCGATAGATAGAGGGGATCGCGGCGTGATCGACCTGCTtgaaaaatggccgtgagaaccattgtttaaccttttgtggcaaaccgatgaa                                                                                               >  2:498472‑M1/1‑97 (MQ=255)                                                    ccTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacag                                                                                           >  1:23368‑M1/1‑93 (MQ=255)                                                     cTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTAAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTATCGGTCTCTGTAAAATGCACGTGAAAACCATTGTTTAACTTTTGGTGGCCaatc                                                                                          >  1:21040/1‑147 (MQ=255)                                                                                        gTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCCCAAGTGCTTTACGCACTGTGGc                                                       >  1:305660/1‑149 (MQ=255)                                                                                                         gcgcGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCGGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTCGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAg                                      <  1:581453/149‑1 (MQ=255)                                                                                                         gcgcGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAg                                      >  1:10769/1‑149 (MQ=255)                                                                                                         gcgcGATGGATAAAGGTCCTCCCTTCCTGATCTCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAg                                      <  2:463050/149‑1 (MQ=255)                                                                                                           gcgATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgcactgtggcttggcgcgaatctgcaggccaaaatttcgc                                    <  2:102572‑M1/149‑112 (MQ=255)                                                                                                            cgATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGcc                                   >  1:349081/1‑149 (MQ=255)                                                                                                                   tAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgcactgtggcttggcgcgaatctgcaggccaaaagttcgcgcagtttc                            >  2:375846‑M1/1‑30 (MQ=255)                                                                                                                           cTCGCGGCGTGATCGCCCTGCTCTCTCAGTCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCGTGGCGCGAATCTGCAGGCCAACATTTCGCGCAGt                    >  2:577497/1‑149 (MQ=255)                                                                                                                            tCGCGGCGTGATCGCCCTGCTtgtaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgcactggggcttggcgctagtctgccggccaaagtttcgcgccgtgtcgagccgcac                   >  2:243375‑M1/1‑21 (MQ=255)                                                                                                                                ggcgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGa               <  1:447266/149‑1 (MQ=255)                                                                                                                                ggcgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGa               <  2:165895/149‑1 (MQ=255)                                                                                                                                             tGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACCGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGCATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAc  >  1:466484/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                  TTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAC  >  NC_000913_3_hsa_tpiA/1726289‑1726577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.