New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 137328419 (0.800)4 (0.180) 4/270 2.6 17.4% coding (63/882 nt) ycjO putative sugar ABC transporter permease
?NC_000913_3_hsa_tpiA = 1373292 20 (0.890)coding (71/882 nt) ycjO putative sugar ABC transporter permease
Rejected: Frequency below/above cutoff threshold.

GCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTG  >  NC_000913_3_hsa_tpiA/1373136‑1373430
                                                                                                                                                    |                                                                                                                                                  
gCCGATCTTTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGC                                                                                                                                                    <  2:109123‑M1/149‑1 (MQ=255)
            actacGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTTACATTTTCCTTTGTCTCTGCTGATTCTCGCGCCCAGCTAATTCCTgctg                                                                                                                                        <  2:150483‑M2/149‑5 (MQ=255)
              gaatccgcacgcgAGCCATAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACCGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCagtaataagctggg                                                                                                                                      <  2:225678‑M1/143‑15 (MQ=255)
               acGCTGCACGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCagtaataagctgggc                                                                                                                                     <  1:369274‑M1/149‑16 (MQ=255)
                              cAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGgtggcgtgg                                                                                                                      <  1:256381/149‑1 (MQ=255)
                                      aCTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGACGTTCAGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGccattgtt                                                                                                              >  2:329367/1‑143 (MQ=255)
                                        tGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCTTGTCGTTCCGATATGCCCTTGGCGCCGCTCCTACCCGCCCCCAGCTTATTACTGCCGGGCGGTCTGATGGCGCGGCCGATGgtgc                                                                                                            >  2:56291/1‑148 (MQ=255)
                                                    tCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATcccca                                                                                                >  2:316134/1‑145 (MQ=255)
                                                            cACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGGAATCAGtttt                                                                                        >  1:38100/1‑149 (MQ=255)
                                                                     caggacaggaATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTg                                                                               <  2:160786/149‑1 (MQ=255)
                                                                         acaggaATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGc                                                                           >  1:280828/1‑149 (MQ=255)
                                                                             gaATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCCGCTTATTACTGCTGGGCGGTCTGGTGGCGGGGCCGATGGTGTCGAATAGCGAAATCAGTTTTTTCAGTCCGCCGCTCaa                                                                       >  2:409608/1‑149 (MQ=255)
                                                                                    cAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCagtaataagctgggcgcga                                                                                                                                 >  1:285228‑M1/1‑65 (MQ=255)
                                                                                    cAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCagtaataagctgggcgcga                                                                                                                                 <  2:285228‑M1/84‑20 (MQ=255)
                                                                                          aCAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCATCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAg                                                          <  1:308585/149‑1 (MQ=255)
                                                                                            aGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGATTTCAGTGTTTTACGTCTGCCGCTGCATCCCAACATCGAGTc                                                        >  1:244242/1‑149 (MQ=255)
                                                                                              gCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCCGTTTTTTACGTCTGCCGCTCCATCTCAAAATCGCGTccg                                                      >  2:223015/1‑147 (MQ=255)
                                                                                              gCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCaa                                                      >  2:298162/1‑149 (MQ=255)
                                                                                                      cAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTttgtt                                              <  1:298162/149‑1 (MQ=255)
                                                                                                                       cccTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCGCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTAtgt                             <  2:280828/149‑1 (MQ=255)
                                                                                                                              cgcTGCTGCTTCTCGCGCCCAGCTTATTGCTCCGGAGCCGTCTGGTGGAGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATc                      <  1:56291/149‑1 (MQ=255)
                                                                                                                                 tgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCctc                   >  2:288305/1‑149 (MQ=255)
                                                                                                                                          tcGCGCCCAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagcctgttcattg                                                                           <  2:285228‑M2/84‑66 (MQ=255)
                                                                                                                                          tcGCGCCCAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagcctgttcattg                                                                           >  1:285228‑M2/1‑19 (MQ=255)
                                                                                                                                              gcCCAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagcctgttcattgcggattcctgtcctgggtttagtgctgttcgatcagttcatccagttttttctggctcgcgtgcagcgt      <  1:369274‑M2/149‑135 (MQ=255)
                                                                                                                                               cccAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagccggttcattgcggattcctgtcctgggtttagtgctgttcgatcagttcatccagttttttatggctcgcgtgcggattc     <  2:225678‑M2/149‑136 (MQ=255)
                                                                                                                                                 cagcaggaattagctgggcgcgagaatcagcagagacaaaggaaaatgtaaacgacctgaaaaaagcctgttcattgcggattcctgtcctgggtttagtgctgttcgatcagttcatccagttttttctggctcgcttgcagcgtagt   <  2:150483‑M1/149‑146 (MQ=255)
                                                                                                                                                 cAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCt   >  2:295368/1‑149 (MQ=255)
                                                                                                                                                 cAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCt   >  1:181856/1‑149 (MQ=255)
                                                                                                                                                  aGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGCTCCCGGCTTCTg  >  2:73331/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTG  >  NC_000913_3_hsa_tpiA/1373136‑1373430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.